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Year 2020, Volume: 35 Issue: 3, 483 - 495, 14.10.2020

İlyas Deligöz , Miray Sokmen

https://doi.org/10.7161/omuanajas.697731
Cited By: 2

Abstract

Potato virus Y (PVY) is one of the most devastating viruses on pepper. Pepper PVY isolates are classified into three pathotypes according to the reactions of pepper genotypes possessing the pvr21 and pvr22 resistance genes at the pvr2-elF4e locus. In this study, in order to determine PVY pathotypes infecting pepper in Samsun province, Turkey, a total of 1012 leaf samples were collected in 2010 and 2011, and these samples were tested by DAS-ELISA using virus-specific polyclonal antiserum. The percentages of PVY infections were found to be 11.9% in 2010 and %5.8 in 2011. Thirteen PVY isolates were propagated and inoculated to pepper differentials to discriminate the pathotypes of PVY. According to reactions of different pepper genotypes and ELISA, nine isolates were found to belong to pathotype 0 (PVY-0) and four isolates to pathotype 1 (PVY-1). The coat protein (CP) gene nucleotide and predicted amino acid sequences of four PVY isolates were obtained. Nucleotide BLAST analysis showed that SÇ-9 (PVY-0), SÇ-84 (PVY-1) and SÇ-87 (PVY-1) isolates had nucleotide sequence similarity (% 97-98) with Tu12.3 isolate from pepper in Turkey and LYE 84.2 isolate from tomato in Spain. In contrast, the isolate SB-63 (PVY-0) had the highest nucleotide identity (% 99) with the isolates belonging to potato NTN strain determined in USA and Poland. Phylogenetic analysis also showed that the pepper isolates SÇ-9, SÇ-84 and SÇ-87 were clustered with the group C isolates of PVY, while the isolate SB-63 was clustered with the group N isolates of PVY.

Project Number

PYO.ZRT1904.10.019

References

  • Aramburu J, Galipienso L, Matas M., 2006. Characterization of potato isolates from tomato crops in northeast Spain, European Journal of Plant Pathology, 115, 247–258.
  • Arlı-Sökmen, M., Mennan, H., Sevik, M.A., Ecevit, O., 2005. Occurrence of viruses in field- grown pepper crops and some of their reservoir weed hosts in Samsun, Turkey, Phytoparasitica, 33 (4), 347-358.
  • Blanco-Urgoiti B., Sánchez, F., Pérez de San Román, C., Dopazo, J., Pons, F., 1998. Potato virus Y group C isolates are a homogenous pathotype but two different genetic strains, Journal of General Virology, 79, 2037-2042.
  • Buzkan, N., Demir, M., Öztekin, V., Mart, C., Çağlar, B. K., Yılmaz., M. A., 2006. Evaluation of the status of capsicum viruses in the main growing regions of Turkey, EPPO Bulletin, 36 (1), 15–19.
  • Buzkan, N., Arpacı, B.B., Moury, B., Fabre, F., 2012. Sürekli virüs dayanıklılığı için Türkiye’de biberler ve potyvirüsler arasındaki genetik ilişkinin analizi. TÜBİTAK TOVAG 109O447 Nolu Proje Sonuç Raporu, 54 s.http://uvt.ulakbim.gov.tr/uvt/index.php?cwid=9&vtadi=TPRJ&ano=145857_e8cbffd43bbcd2dcce840b488d80db83 (erişim 13.03.2013).
  • Buzkan, N., Arpacı, B.B., Görsoy, G., Zencirkıran, M., Moury, B., 2015. Genetic variability of Potato virus Y (PVY) and Tobacco etch virus (TEV) from naturally infected pepper fields in the Hatay region of Turkey. Archives of Phytopathology and Plant Protection. 48 (7), 588–600.
  • Caranta, C., Thabuis, A., Palloix, A., 1999. Development of a CAPS marker for the Pvr4 locus: a tool for pyramiding Potyvirus resistance genes in pepper. Genome / National Research Council Canada. 42(6), 1111-6. Cardin, L., Moury, B., 2008. First report of Potato virus Y in Nicotiana mutabilis in France. Plant Disease, 92, 312.
  • Chikh Ali M., Maoka T., Natsuaki T., Natsuaki K. T., 2010. PVYNTN-NW, a novel recombinant strain of Potato virus Y predominating in potato fields in Syria. Plant Pathology, 59: 31 – 41.
  • Clark, M.F., Adams, A.N., 1977. Characteristics of microplate metod of Enzyme-linked Immunosorbent Assay for the detection of plant viruses, Journal of General Virology, 34, 475-483.
  • Çelik, N., Özalp, R., Göçmen, M., 2012. Antalya ili örtü altı biber yetiştiriciliğinde Patates Y Virüsü (PVY) patotiplerinin belirlenmesi ve bazı biber çeşitlerinin PVY’ye karşı reaksiyonları. Bitki Koruma Bülteni, 52 (3). 235-246.
  • Echer, M., Costa, C.P., 2002. Reaction of sweet pepper to the Potato Virus Y (PVYm)1, Scientia Agricola, 59 (2), 309-314.
  • Gebre-Selassie, K., Marchoux, G., Delecolle, B., Pochard, E., 1985. Variabilite naturelle des souches du virus Y de la porn me de terre dans les cultures de piment du Sud- Est de la France. Caracterisation et classification en pathotypes, Agronomie, 5 (7), 621-630.
  • Glais,, L., Tribodet, M., Kerlan, C., 2002. Genomic variability in Potato potyvirus Y (PVY): evidence that PVYNW and PVYNTN variants are single to multiple recombinants between PVYO and PVYN isolates, Archives of Virology, 147, 363–378.
  • Grzela, R., Szolajska, E., Ebel, C., Madern, D., Favier, A., Wojtal, I., Zagorski, W., Chroboczek, J., 2008. Virulence factor of Potato virus Y, genome-attached terminal protein VPg, is a highly disordered protein, Journal of Biological Chemistry, 283(1), 213-21.
  • Gugerli, P., Fries, P., 1983. Characterization of monoclonal antibodies to Potato virus Y and their use for virus detection, Journal of General Virology, 64, 2471-2477.
  • Hamalainen, J.H., Watanabe, K.N., Valkonen, J.P.T., Arihara, A., Plaisted, R.L., Pehu, E., Miller, L., Slack, S.A., 1997. Mapping and marker-assisted selection for a gene for extreme resistance to Potato virus Y, Theoretical and Applied Genetics, 94, 192-197. Heldak, J., 1997. Symptomatological and serological evaluation of Potato virus Y (PVY) isolates in relation to potato tuber necrosic ringspot disease Agriculture (Poľnohospodárstvo), 43:1, 18-32.
  • Jordan, R. L., Hammond, J., 1991. Comparison and differentiation of Potyvirus isolates and identification of strain-, virus-, subgroup- specific, and Potyvirus group-common epitopes using monoclonal antibodies, Journal of General. Virology, 72, 25-36.
  • Karasev A. V., Gray S. M., 2013. Continuous and emerging challenges of Potato virus Y in potato. Annual Review of Phytopathology, 51: 571 – 86.
  • Kazinczi, G., Kovács, J., Takács, A.P., Horváth, J., Gáborjányi. R., 2003. Susceptibility of Capsicum breeding lines to NTN strain of Potato virus Y (PVY(NTN)) and Obuda pepper virus (ObPV), Communications in Agricultural and Applied Biological Sciences, 68, 555-9
  • Kerlan, C., 2006. Potato Virus Y. AAB Descriptions of Plants Viruses, 414.
  • Kerlan, C., Moury, B., 2008. Potato Virus Y. In Encyclopedia of Virology, 287-296. Edited by B.W.J. Mahy & M.H.V. van Regenmortel. Oxford: Academic Press.
  • Llave, C., Martinez, B., Diaz-Ruiz, J.R., López-Abella, D., 1999. Serological analysis and coat protein sequence determination of Potato virus Y (PVY) pepper pathotypes and differentiation from other PVY strains, European Journal of Plant Pathology, 105 (9), 847-857.
  • Moury, B., Morel, C., Johansen, E., Jacquemond, M., 2002. Evidence for diversifying selection in Potato virus Y and in the coat protein of other potyviruses, Journal of General Virology, 83, 2563–2573.
  • Ogawa, T., Tomitaka, Y., Nakagawa, A., Ohshima, K., 2008. Genetic structure of a population of Potato virus Y inducing potato tuber necrotic ringspot disease in Japan; comparison with North American and European populations, Virus Research, 131, 199–212.
  • Palloix, A., Abak, K., Gognalons, P., Daubeze, A.M., Güldür, M.E., Memouchi, G., Selassie, K.G., 1994. Virus diseases işnfecting pepper crops in Türkiye. 9th Congress of the Mediteranean Phytopathological Union, September 18-24, Kuşadası-Aydın, Türkiye, 469-472.
  • Palloix A., Kyle M., 1995. Proposal revision of gene nomenclature for Potyvirus resistance genes in Capsicum sp., Capsicum and Eggplant Newsletter, 14, 26-29.
  • Revers, F., Le Gall, O., Candresse, T., Le Romancer, M., Dunez, J., 1996. Frequent occurrence of recombinant Potyvirus isolates, Journal of General Virology, 77, 1953–1965.
  • Quenouille J., Vassilakos N., Moury B., 2013. Potato virus Y: a major crop pathogen that has provided major insights into the evolution of viral pathogenicity. Molecular Plant Pathology, 14: 439 – 452.
  • Riechmann, J.L., Lain, S., Garcia, J.A., 1992. Review Article: Highlights and prospects of Potyvirus molecular biology, Journal of General Virology, 73, 1-16.
  • Rigotti, S., Gugerli, P., 2007. Rapid identification of Potato virus Y strains by one-step triplex RT-PCR, Journal of Virological Methodsi 140, 90–94.
  • TUİK, 2018. Türkiye İstatistik Kurumu. www.tuik.gov.tr. URL: https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr (Erişim tarihi:10.01.2019).
  • Urcuqui-Inchima, S., Haenni, A.L., Bernardi, F., 2001. Potyvirus proteins: a wealth of functions, Virus Research, 74, 157-175.
  • Varveri, C., 2000. Potato Y potyvirus detection by ımmunological and molecular techniques in plants and aphids. Phytoparasitica. 28: 141-148.

Samsun ilinde biber alanlarında enfeksiyon oluşturan Potato virus Y -patotiplerinin belirlenmesi ve moleküler karakterizasyonu

Year 2020, Volume: 35 Issue: 3, 483 - 495, 14.10.2020

İlyas Deligöz , Miray Sokmen

https://doi.org/10.7161/omuanajas.697731
Cited By: 2

Abstract

Potato virus Y (PVY), biberde en fazla zarar oluşturan virüs türleri arasında yer almaktadır. Biber izolatları, pvr2- eIF4E lokusundaki, pvr21 ve pvr22 dayanıklılık genlerine sahip biber genotiplerinin verdikleri reaksiyonlara göre üç ayrı patotip altında sınıflandırılmaktadır. Bu çalışmada, Samsun ilinde biberde enfeksiyon oluşturan PVY patotiplerinin belirlenmesi amacıyla 2010 ve 2011 yıllarında toplam 1012 yaprak örneği toplanmış ve daha sonra örnekler, PVY’ye spesifik poliklonal antiserum kullanılarak DAS-ELISA yöntemi ile test edilmiştir. Test sonucunda, 2010 yılında alınan örneklerin %11.9’unun, 2011 yılında ise %5.8’inin PVY ile enfekteli olduğu belirlenmiştir. PVY ile enfekteli olarak belirlenen 90 örneğin %26.6’sının diğer virüslerle (Cucumber mosaic virus, Tomato spotted wilt virus, Pepper mild mottle virus) karışık enfekteli olduğu saptanmıştır. Test edilen diğer virüs türleri ile karışık-enfekteli olmayan ve çoğaltımı sağlanan 13 PVY izolatı, patotip ayrım setindeki bitkilere inokule edilmiştir. Bu bitkilerin reaksiyonlarına ve ELISA sonuçlarına göre izolatların 9’nun patotip 0 (PVY-0)’a, 4’ünün ise patotip 1 (PVY-1)’e ait olduğu ortaya konmuştur. Ayrıca PVY-0 ve PVY-1 patotiplerine ait ikişer izolatın kılıf protein (CP) gen bölgesinin nükleotid dizileri elde edilmiştir. Nükleotid BLAST analizi, PVY-0 patotipine ait SÇ-9 izolatı ve PVY-1 patotipine ait SÇ-84 ve SÇ-87 nolu izolatların, Türkiye orjinli Tu12.3 biber izolatı ve İspanya orjinli LYE 84.2 domates izolatı ile genetik benzerlik (% 97-98) gösterdiğini; PVY-0 patotipine ait SB-63 nolu izolatın ise ABD ve Polonya’da patateste belirlenen NTN ırkına ait izolatlar ile % 99 benzer olduğunu ortaya koymuştur.

Keywords

Samsun biber PVY patotip

Supporting Institution

Ondokuzmayıs Üniversitesi

Project Number

PYO.ZRT1904.10.019

References

  • Aramburu J, Galipienso L, Matas M., 2006. Characterization of potato isolates from tomato crops in northeast Spain, European Journal of Plant Pathology, 115, 247–258.
  • Arlı-Sökmen, M., Mennan, H., Sevik, M.A., Ecevit, O., 2005. Occurrence of viruses in field- grown pepper crops and some of their reservoir weed hosts in Samsun, Turkey, Phytoparasitica, 33 (4), 347-358.
  • Blanco-Urgoiti B., Sánchez, F., Pérez de San Román, C., Dopazo, J., Pons, F., 1998. Potato virus Y group C isolates are a homogenous pathotype but two different genetic strains, Journal of General Virology, 79, 2037-2042.
  • Buzkan, N., Demir, M., Öztekin, V., Mart, C., Çağlar, B. K., Yılmaz., M. A., 2006. Evaluation of the status of capsicum viruses in the main growing regions of Turkey, EPPO Bulletin, 36 (1), 15–19.
  • Buzkan, N., Arpacı, B.B., Moury, B., Fabre, F., 2012. Sürekli virüs dayanıklılığı için Türkiye’de biberler ve potyvirüsler arasındaki genetik ilişkinin analizi. TÜBİTAK TOVAG 109O447 Nolu Proje Sonuç Raporu, 54 s.http://uvt.ulakbim.gov.tr/uvt/index.php?cwid=9&vtadi=TPRJ&ano=145857_e8cbffd43bbcd2dcce840b488d80db83 (erişim 13.03.2013).
  • Buzkan, N., Arpacı, B.B., Görsoy, G., Zencirkıran, M., Moury, B., 2015. Genetic variability of Potato virus Y (PVY) and Tobacco etch virus (TEV) from naturally infected pepper fields in the Hatay region of Turkey. Archives of Phytopathology and Plant Protection. 48 (7), 588–600.
  • Caranta, C., Thabuis, A., Palloix, A., 1999. Development of a CAPS marker for the Pvr4 locus: a tool for pyramiding Potyvirus resistance genes in pepper. Genome / National Research Council Canada. 42(6), 1111-6. Cardin, L., Moury, B., 2008. First report of Potato virus Y in Nicotiana mutabilis in France. Plant Disease, 92, 312.
  • Chikh Ali M., Maoka T., Natsuaki T., Natsuaki K. T., 2010. PVYNTN-NW, a novel recombinant strain of Potato virus Y predominating in potato fields in Syria. Plant Pathology, 59: 31 – 41.
  • Clark, M.F., Adams, A.N., 1977. Characteristics of microplate metod of Enzyme-linked Immunosorbent Assay for the detection of plant viruses, Journal of General Virology, 34, 475-483.
  • Çelik, N., Özalp, R., Göçmen, M., 2012. Antalya ili örtü altı biber yetiştiriciliğinde Patates Y Virüsü (PVY) patotiplerinin belirlenmesi ve bazı biber çeşitlerinin PVY’ye karşı reaksiyonları. Bitki Koruma Bülteni, 52 (3). 235-246.
  • Echer, M., Costa, C.P., 2002. Reaction of sweet pepper to the Potato Virus Y (PVYm)1, Scientia Agricola, 59 (2), 309-314.
  • Gebre-Selassie, K., Marchoux, G., Delecolle, B., Pochard, E., 1985. Variabilite naturelle des souches du virus Y de la porn me de terre dans les cultures de piment du Sud- Est de la France. Caracterisation et classification en pathotypes, Agronomie, 5 (7), 621-630.
  • Glais,, L., Tribodet, M., Kerlan, C., 2002. Genomic variability in Potato potyvirus Y (PVY): evidence that PVYNW and PVYNTN variants are single to multiple recombinants between PVYO and PVYN isolates, Archives of Virology, 147, 363–378.
  • Grzela, R., Szolajska, E., Ebel, C., Madern, D., Favier, A., Wojtal, I., Zagorski, W., Chroboczek, J., 2008. Virulence factor of Potato virus Y, genome-attached terminal protein VPg, is a highly disordered protein, Journal of Biological Chemistry, 283(1), 213-21.
  • Gugerli, P., Fries, P., 1983. Characterization of monoclonal antibodies to Potato virus Y and their use for virus detection, Journal of General Virology, 64, 2471-2477.
  • Hamalainen, J.H., Watanabe, K.N., Valkonen, J.P.T., Arihara, A., Plaisted, R.L., Pehu, E., Miller, L., Slack, S.A., 1997. Mapping and marker-assisted selection for a gene for extreme resistance to Potato virus Y, Theoretical and Applied Genetics, 94, 192-197. Heldak, J., 1997. Symptomatological and serological evaluation of Potato virus Y (PVY) isolates in relation to potato tuber necrosic ringspot disease Agriculture (Poľnohospodárstvo), 43:1, 18-32.
  • Jordan, R. L., Hammond, J., 1991. Comparison and differentiation of Potyvirus isolates and identification of strain-, virus-, subgroup- specific, and Potyvirus group-common epitopes using monoclonal antibodies, Journal of General. Virology, 72, 25-36.
  • Karasev A. V., Gray S. M., 2013. Continuous and emerging challenges of Potato virus Y in potato. Annual Review of Phytopathology, 51: 571 – 86.
  • Kazinczi, G., Kovács, J., Takács, A.P., Horváth, J., Gáborjányi. R., 2003. Susceptibility of Capsicum breeding lines to NTN strain of Potato virus Y (PVY(NTN)) and Obuda pepper virus (ObPV), Communications in Agricultural and Applied Biological Sciences, 68, 555-9
  • Kerlan, C., 2006. Potato Virus Y. AAB Descriptions of Plants Viruses, 414.
  • Kerlan, C., Moury, B., 2008. Potato Virus Y. In Encyclopedia of Virology, 287-296. Edited by B.W.J. Mahy & M.H.V. van Regenmortel. Oxford: Academic Press.
  • Llave, C., Martinez, B., Diaz-Ruiz, J.R., López-Abella, D., 1999. Serological analysis and coat protein sequence determination of Potato virus Y (PVY) pepper pathotypes and differentiation from other PVY strains, European Journal of Plant Pathology, 105 (9), 847-857.
  • Moury, B., Morel, C., Johansen, E., Jacquemond, M., 2002. Evidence for diversifying selection in Potato virus Y and in the coat protein of other potyviruses, Journal of General Virology, 83, 2563–2573.
  • Ogawa, T., Tomitaka, Y., Nakagawa, A., Ohshima, K., 2008. Genetic structure of a population of Potato virus Y inducing potato tuber necrotic ringspot disease in Japan; comparison with North American and European populations, Virus Research, 131, 199–212.
  • Palloix, A., Abak, K., Gognalons, P., Daubeze, A.M., Güldür, M.E., Memouchi, G., Selassie, K.G., 1994. Virus diseases işnfecting pepper crops in Türkiye. 9th Congress of the Mediteranean Phytopathological Union, September 18-24, Kuşadası-Aydın, Türkiye, 469-472.
  • Palloix A., Kyle M., 1995. Proposal revision of gene nomenclature for Potyvirus resistance genes in Capsicum sp., Capsicum and Eggplant Newsletter, 14, 26-29.
  • Revers, F., Le Gall, O., Candresse, T., Le Romancer, M., Dunez, J., 1996. Frequent occurrence of recombinant Potyvirus isolates, Journal of General Virology, 77, 1953–1965.
  • Quenouille J., Vassilakos N., Moury B., 2013. Potato virus Y: a major crop pathogen that has provided major insights into the evolution of viral pathogenicity. Molecular Plant Pathology, 14: 439 – 452.
  • Riechmann, J.L., Lain, S., Garcia, J.A., 1992. Review Article: Highlights and prospects of Potyvirus molecular biology, Journal of General Virology, 73, 1-16.
  • Rigotti, S., Gugerli, P., 2007. Rapid identification of Potato virus Y strains by one-step triplex RT-PCR, Journal of Virological Methodsi 140, 90–94.
  • TUİK, 2018. Türkiye İstatistik Kurumu. www.tuik.gov.tr. URL: https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr (Erişim tarihi:10.01.2019).
  • Urcuqui-Inchima, S., Haenni, A.L., Bernardi, F., 2001. Potyvirus proteins: a wealth of functions, Virus Research, 74, 157-175.
  • Varveri, C., 2000. Potato Y potyvirus detection by ımmunological and molecular techniques in plants and aphids. Phytoparasitica. 28: 141-148.
There are 33 citations in total.

Details

Primary Language Turkish
Journal Section Anadolu Tarım Bilimleri Dergisi
Authors

İlyas Deligöz 0000-0003-3646-300X

Miray Sokmen 0000-0002-4963-9070

Project Number PYO.ZRT1904.10.019
Publication Date October 14, 2020
Acceptance Date September 14, 2020
Published in Issue Year 2020 Volume: 35 Issue: 3

Cite

APA Deligöz, İ., & Sokmen, M. (2020). Samsun ilinde biber alanlarında enfeksiyon oluşturan Potato virus Y -patotiplerinin belirlenmesi ve moleküler karakterizasyonu. Anadolu Tarım Bilimleri Dergisi, 35(3), 483-495. https://doi.org/10.7161/omuanajas.697731
AMA Deligöz İ, Sokmen M. Samsun ilinde biber alanlarında enfeksiyon oluşturan Potato virus Y -patotiplerinin belirlenmesi ve moleküler karakterizasyonu. ANAJAS. October 2020;35(3):483-495. doi:10.7161/omuanajas.697731
Chicago Deligöz, İlyas, and Miray Sokmen. “Samsun Ilinde Biber alanlarında Enfeksiyon oluşturan Potato Virus Y -Patotiplerinin Belirlenmesi Ve moleküler Karakterizasyonu”. Anadolu Tarım Bilimleri Dergisi 35, no. 3 (October 2020): 483-95. https://doi.org/10.7161/omuanajas.697731.
EndNote Deligöz İ, Sokmen M (October 1, 2020) Samsun ilinde biber alanlarında enfeksiyon oluşturan Potato virus Y -patotiplerinin belirlenmesi ve moleküler karakterizasyonu. Anadolu Tarım Bilimleri Dergisi 35 3 483–495.
IEEE İ. Deligöz and M. Sokmen, “Samsun ilinde biber alanlarında enfeksiyon oluşturan Potato virus Y -patotiplerinin belirlenmesi ve moleküler karakterizasyonu”, ANAJAS, vol. 35, no. 3, pp. 483–495, 2020, doi: 10.7161/omuanajas.697731.
ISNAD Deligöz, İlyas - Sokmen, Miray. “Samsun Ilinde Biber alanlarında Enfeksiyon oluşturan Potato Virus Y -Patotiplerinin Belirlenmesi Ve moleküler Karakterizasyonu”. Anadolu Tarım Bilimleri Dergisi 35/3 (October 2020), 483-495. https://doi.org/10.7161/omuanajas.697731.
JAMA Deligöz İ, Sokmen M. Samsun ilinde biber alanlarında enfeksiyon oluşturan Potato virus Y -patotiplerinin belirlenmesi ve moleküler karakterizasyonu. ANAJAS. 2020;35:483–495.
MLA Deligöz, İlyas and Miray Sokmen. “Samsun Ilinde Biber alanlarında Enfeksiyon oluşturan Potato Virus Y -Patotiplerinin Belirlenmesi Ve moleküler Karakterizasyonu”. Anadolu Tarım Bilimleri Dergisi, vol. 35, no. 3, 2020, pp. 483-95, doi:10.7161/omuanajas.697731.
Vancouver Deligöz İ, Sokmen M. Samsun ilinde biber alanlarında enfeksiyon oluşturan Potato virus Y -patotiplerinin belirlenmesi ve moleküler karakterizasyonu. ANAJAS. 2020;35(3):483-95.

Cited By

Türkiye’de biberde enfeksiyon oluşturan bazı virüslerin belirlenmesi
ANADOLU JOURNAL OF AGRICULTURAL SCIENCES
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Bazı Biber Çeşit ve Islah Hatlarının Potato virus Y (PVY)’nin 0 ve 1 Patotiplerine Reaksiyonlarının ve PVY’ye Resesif Dayanıklılıkta Rol Oynayan Genlerinin Araştırılması
Türk Tarım ve Doğa Bilimleri Dergisi
https://doi.org/10.30910/turkjans.981393
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