Enginarda [Cynara cardunculus var. scolymus (L.) Fiori] Kuraklık Stresi Ajanı Olarak Mannitol Kullanımı

Yıl 2023, Cilt: 38 Sayı: 3, 637 - 656, 26.10.2023

Tugce Ozsan Kılıc , Timur Tongur , Ahmet Naci Onus

https://doi.org/10.7161/omuanajas.1353386

Öz

Asteraceae familyasının önemli üyelerinden biri olan enginar [Cynara cardunculus var. scolymus (L.) Fiori], eski çağlardan beri mutfak ve sağlık açısından faydaları nedeniyle insanlar tarafından tüketilmektedir. Küresel tarımsal üretim ve üretim verimliliği, başta kuraklık olmak üzere olumsuz çevre koşulları nedeniyle sekteye uğramaktadır. Bitki verimliliğini olumsuz etkileyen kuraklık stresine dayanıklı bitkilerin kullanılması, ıslah çalışmalarının teşvik edilmesi açısından büyük önem taşımaktadır. Kuraklık stresi altında bitkilerde meydana gelen değişiklikleri anlamak çok önemlidir. Bu çalışmada enginar tohumları ilk olarak yaygın olarak kullanılan kuraklık stres ajanı olan mannitol içerisinde üç farklı konsantrasyonda (50, 100 ve 250 mg L-1) 8 saat süreyle bekletilmiş ve daha sonra elde edilen fideler 15 gün aralıklarla aynı mannitol konsantrasyonlarına tabi tutulmuştur. Daha sonra üç enginar çeşidinde (Sakız, Bayrampaşa ve Olympus F1) yaprak sayısı, kök uzunluğu, gövde uzunluğu, bitki boyu, klorofil, prolin, yaprağa bağlı su içeriği ve toplam fenol-antioksidan içeriği gibi bazı tohum çimlenmesi ve vejetatif büyüme parametreleri kuraklık stresi altında araştırılmıştır. Sonuçlar, kuraklık stresinin enginar tohumları ve fideleri üzerindeki etkisinin çeşitlere, ön işleme ve uygulama konsantrasyonlarına bağlı olarak değiştiğini ortaya koymuştur.

Anahtar Kelimeler

Abiyotik stres, Stres ajanı, Büyüme parametreleri, Toplam fenol içeriği, Toplam antioksidan içeriği

Using Mannitol as Drought Stress Agent on Globe Artichoke [Cynara cardunculus var. scolymus (L.) Fiori]

Öz

The globe artichoke [Cynara cardunculus var. scolymus (L.) Fiori], one of the special members of the Asteraceae family, has been consumed by people for their culinary and health advantages since ancient times. Global agricultural output and production efficiency are both hampered by adverse environmental conditions, notably drought. In order to promote breeding studies, it is crucial to use plants that are adaptable to drought stress, which negatively impacts plant productivity. It is crucial to understand the changes taking place in plants under drought stress. In the present study, artichoke seeds were initially kept in mannitol, which is a commonly used drought stress agent, in three different concentrations (50, 100, and 250 mg L-1) for 8 hours and later on obtained seedlings were subjected to same mannitol concentrations with 15 days intervals. Later on some seed germination and vegetative growth parameters, such as number of leaves, root length, stem height, plant height, chlorophyll, proline, leaf-related water contents, and total phenol-antioxidant contents in three globe artichoke cultivars (Sakız OP, Bayrampaşa OP, and Olympus F1) were investigated under drought stress. Results revealed that the impact of drought stress on artichoke seeds and seedlings varied depending on cultivars and pre-treatment and treatment concentrations.

Anahtar Kelimeler

Abiotic stress, Stress agent, Growth parameters, Total phenol content, Total antioxidant content

Kaynakça

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