The Effect Of Omega-3 Solution In The Prevention Of Epidural Fibrosis Formation In Experimental Rabbit Hemilaminotomy Model

Year 2010, Volume: 27 Issue: 1, 16 - 19, 25.01.2012

Cengiz Çokluk , Kerameddin Aydın , Levent Tümkaya

Abstract

The aim of this experimental study was to evaluate the effect of omega-3 fatty acids solution in the prevention of the forming of peridural fibrotic tissue and adhesion in a rabbit hemilaminotomy model. This new experimental model was previously developed by the first author of this study. Twenty-one New Zealand white rabbits were used for this experiment. Seven of them were randomly selected as Control group and was named as Group I rabbits. The rabbits in this group were not operated and treated. The second group was Group II including 7 rabbits. This group was named as hemilaminotomy and untreated rabbits. Group III included 7 rabbits and named as Omega-3 treated group after hemilaminotomy procedure. In this group (Group III), 250 mg omega-3 solution was put in the hemilaminotomy area before closing the skin and fascia. Four weeks later from the operation rabbits were sacrificied by perfusion with 10% neutral buffered formalin solution. The lumbar spines were removed and immersed in 10% neutral buffered formalin for approximately 24 hours. Then each specimen was decalcified in 5% formic acid for approximately 3 weeks. Specimens were cut coronally for gross inspection. Peridural scar tissue on gross inspection in coronal sectioned specimens was graded into three grades. Grade I epidural scar tissue was scored with 1 point, Grade II with 2 point and Grade III with 3 point. The average score of hemilaminotomy sites without treatment was found as 2, 6 ± 0, 51. In the other hand the average score of hemilaminotomy sites with omega-3 fatty acids treatment was found as 1, 9 ± 0, 56. The difference between the treatment and non-treatment group was statistically significant. Omega-3 fatty acids solution seems an effective treatment modality in the prevention of peridural excessive scar tissue formation in the rabbit hemilaminotomy model.

Keywords

Peridural Fibrosis, Fatty Acids, Hemilaminotomy, Omega-3, Lumbar spine, Rabbit

References

• Abitbol, J.J., Lincoln T.L., Lind B.I., Amiel D., Akeson W.H., Garfin S.R., 1994. Preventing postlaminectomy adhesion: a new experimental model. Spine. 19, 1809-1814
• Cokluk, C., Aydin, K., 2005. Experimental rabbit hemilaminotomy model in the evaluation of peridural fibrosis: A minimally invasive peridural fibrosis model. Minim. Invas. Neurosurg. 48, 235-239
• Dogulu, F., Kurt, G., Emmez, H., Erdem, O., Memis, L., Baykaner, K., Ceviker, N., 2003. Topical mitomycin C-induced inhibition of postlaminectomy peridural fibrosis in rabbits. J. Neurosurg. 99, 76-79
• Gabriel, E.M., Friedman, A.H., 1996. The failed back surgery syndrome. In: Wilkins RH, Rengachary DD, editors. Neurosurgery. McGraw-Hill, New York, 3863–3870
• Minamide, A., Ilashizume, H., Yoshida, M., 1999. Effects of basic fibroblast growth factor on spontaneous resorption of hemiated intervertebral discs: an experimental study in the rabbit. Spine. 24, 940-945
• Nussbaum, C.E., McDonald, J.V., Baggs, R.B., 1990. Use of Vicryl (polyglactin 910) mesh to limit epidural scar formation after laminectomy. Neurosurgery. 26, 649-654
• Robertson, J.T., Meric, A.L., Dohan, F.C. Jr, Schweitzer, J.B., Wujek, J.R., Ahmad, S., 1993. The reduction of postlaminectomy peridural fibrosis in rabbits by a carbohydrate polymer. J. Neurosurg. 79, 89-95