Effects of leptin on histomorphometry of liver in high-fat diet fed obese rats

Year 2013, Volume: 30 Issue: 2, 141 - 145, 31.07.2013

Elfide Gizem Kıvrak Işınsu Aydın Gamze Tümentemur B. Zuhal Altunkaynak , Süleyman Kaplan

Abstract

Obesity, a condition that is characterized by the excessive accumulation and storage of body fat, is one of the most significant and common diseases of today’s world and it is
measured by a method called body mass index (BMI). Obesity can result in many serious diseases such as, hypertension, diabetes, cardiovascular diseases and hyperlipidemia.
Non-alcoholic fatty liver disease is one cause of a fatty liver that occurs when fat is deposited in the liver not due to the excessive alcohol use. Histopathological findings of fatty liver are seen in hepatocytes within the forms of micro-vesicular and macrovesicular steatosis or sometimes housing fat vacuoles. Leptin, one of the most important adipose-derived hormones and among the best-known hormone markers for obesity plays a key role in regulating stable body weight and is synthesized and released by the fat cells in adipose tissue. The aim of this study was to detect the effects of high fat diet induced obesity and leptin on liver by using physical disector and Cavalieri methods.

Keywords

High fat diet Leptin; Non-alcoholic fatty liver disease; Obesity Rat

References

• Altunkaynak, B. Z., Özbek, E., 2009. Overweight and structural alterations of the liver in female rats fed a high-fat diet: A stereological and histological study. Turk. J. Gastroenterol. 20, 93-103.
• Altunkaynak, B.Z, Onger, M.E., Altunkaynak, M.E., Ayrancı, E., Canan, S., 2012. A brief introduction to stereology and sampling strategies: Basic concepts of stereology. NeuroQuantology. 10, 31-43.
• Auwerx, J., Staels, B., 1998. Leptin. Lancet. 351, 737-742.
• Büyükokuroğlu, M. E., Göçer, F., Ünüvar, N., 1999. Leptin. AÜTD. 3, 1-5.
• Erkal, T., 2008. Non alkolik yağlı karaciğer yağlı karaciğer hastalığının siroz panelindeki yeri nedir? Uzmanlık tezi. T.C. Sağlik Bakanliği Haseki Eğitim ve Araştirma Hastanesi, İç Hastaliklari kliniği.
• Gilat, T., Leikin-Frenkel, A., Goldiner, I., Juhel, C., Lafont, H., Gobbi, D., Konikoff, F.M., 2003. Prevention of diet-induced fatty liver in experi- mental animals by the oral administration of a fatty acid bile acid conjugate (FABAC). Hepatology; 38, 436-442.
• Gültürk, S., İmir, G., 2006. Leptin ve nöroendokrin düzenleme. Journal of Adnan Menderes Universty Medical Faculty. 7, 49-54.
• Halaas, J.L., Gajiwala, K.S., Maffei, M., Cohen, S.L., Chait, B.T., Rabinowitz, D., Lallone, R.L., Burley, S.K., Friedman, J.M., 1995. Weight- reducing effects of the plasma protein encoded by the obese gene. Science. 269, 543-546.
• Hekimoğlu, A., 2006. Leptin ve fizyopatolojik olaylardaki rolü. Dicle Tıp Dergisi. 33, 259-267.
• İşbilen, B., Arı, Z., Var. O., Onur, E., Uyanık, B. S., 2007. Yüksek yağ içeren diyet ile beslenen ratlarda DHEAS’ın leptin, lipid profili ve endotel fonksiyonu üzerine etkileri. F.Ü. Sağ. Bil. Derg. 21, 109-116.
• Kalaycioglu, A., Ucuncu, Y., Altunkaynak, B.Z., Altunkaynak, M.E., Gundogdu, C., Keles,O.N., Unal, B., Kaplan,S., 2013. Stereological evalu- ation of the kidneys of anencephalic and normal fetuses. Renal Fail. 35, 110-117.
• Kiki, I., Altunkaynak, B.Z., Altunkaynak, M.E., Vuraler, O., Unal, D., Kaplan, S., 2007. Effect of high fat diet on the volume of liver and quan- titative feature of kupffer cells in the female rat: A stereological and ultrastructural study. Obes. Surg. 17, 1381-1388.
• Nicol, C.J., Adachi, M., Akiyama, T.E., Gonzalez, F.J., 2005. PPAR gamma in endothelial cells influences high fat diet-induced hypertension. Am. J. Hypertens. 18, 549-56.
• Sahin, B., Emirzeoglu, M., Uzun, A., Incesu, L., Bek, Y., Bilgic, S., Kaplan, S., 2003. Unbiased estimation of the liver volume by the Cavalieri principle using magnetic resonance images. Eur. J. Radiol. 47, 164-170.
• Sonsuz, A., 2007. Nonalkolik Karaciğer Yağlanması. İ.Ü. Cerrahpaşa Tıp Fakültesi Sürekli Tıp Eğitimi Etkinlikleri. 58, 91-98.
• Turkish Society for Stereology, www.türkstreoloji.org. Accessed: 27.03.2013.
• Üstün, Y. B., Köksal, E., 2013. Obezite ve anestezi (J. Exp. Clin. Med., in press).
• Vamecq, J., Vallee, L., de la Porte, P.L., Fontaine, M., de Craemer, D., van den Branden, C., Lafont, H., Grataroli, R., Nalbone, G., 1993. Effect of various n-3/n-6 fatty acid ratio contents of high fat diets on rat liver and heart peroxisomal and mitochondrial beta-oxidation. Biochem. Biophys. Acta. 1170, 151-156.
• Yavuz, R., Tontuş, H. Ö., 2013. Erişkin, adolesan ve çocukluk yaş grubunda obeziteye klinik yaklaşım J. Exp. Clin. Med (in press).