Stereological Examination of the Effect on the Hippocampal Dentate Gyrus Granule Cells Number of Rats Subjected to Wireless Internet During Prenatal Period

Year 2022, Volume: 39 Issue: 3, 597 - 603, 30.08.2022

Büşranur Özalper , Murat Çetin Ragbetli , Tahir Cakir

Abstract

As a result of industrialisation and the many recent advances in technology, we are being intensely subjected to non-ionised radiation sources. As such, the effects of non-ionised radiation on human tissue are now being researched. In this study, the effects of Wi-Fi waves found in some third-generation mobile phones to facilitate internet connection—on the brain hippocampal dentate gyrus granule cell quantity of rats during the prenatal period was stereologically examined. During the study, mated albino rats were subjected to Wi-Fi throughout pregnancy. A month after giving birth, six rats from each group, for a total of 12 rats, was sacrificed under perfusion and anaesthesia. Their skulls were opened and brains were removed to undergo routine checks in which the granule cells were counted. Strategic sections were defined, and every 45th section couple (with a thickness of 5 µm) was taken and dyed with haematoxylin and eosin (H&E) and cresyl violet stain. The granule cells were counted using a combination of the stereological dissector method and Cavalier’s principle. The results were analysed using the Mann–Whitney U test. No statistically significant difference has been observed between the groups (p > 0.05). The findings were discussed in light of the relevant literature, and it was determined that throughout pregnancy, Wi-Fi modem device did result in any changes in the dentate gyrus granule cell count of the hippocampus of postnatal rats’ brains.

Keywords

Wi-fi, Cerebellar Granule Cells Numbers, Rats Prenatal Period

Supporting Institution

This research, which taken from Buşranur Özalper's master's thesis, was supported by Van Yuzuncu Yil University Scientific Research Projects Unit

Project Number

TYL-2016-5331

Thanks

Thanks to Van Yüzüncü Yil University Scientific Research Projects Unit

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