Investigation of new biomarkers that can be used for the diagnosis of asymptomatic diabetic peripheral artery disease (PAD): Serum netrin-1 levels can be a potential biomarker for the diagnosis of PAD

Year 2024, Volume: 41 Issue: 1, 143 - 151, 29.03.2024

Ahmet Kor , Ali Altınok , Fikret Akyürek , Tahire Betül Kor , Fatih Çölkesen , Süleyman Baldane , Suleyman Ipekci , Bahadır Öztürk , Ali Unlu , Kamile Marakoğlu , Levent Kebapcilar

Abstract

Diabetic peripheral arterial disease (PAD) is strongly associated with cardiovascular and cerebrovascular diseases that contribute to a high risk of mortality and morbidity. Diagnostic tests that can predict the development of diabetic PAD in the asymptomatic period are inadequate, and new potential biomarkers are needed. This study was prepared to investigate the diagnostic values of serum netrin-1, endothelin-1, oxidative stress parameters, and L-arginine/methylarginine derivatives for diabetic asymptomatic PAD. 386 type-2 diabetes mellitus patients who were clinically asymptomatic for PAD were screened for PAD by ankle-brachial index (ABI) measurement. At the same time, 40 patients with PAD were included in the study group, and 41 patients without PAD were included in the control group. Ready-to-use commercial kits were used to calculate serum netrin-1, endothelin-1, and oxidative stress markers. Serum netrin-1 median values were found to be significantly higher in the group with diabetic PAD than in the group without (OR [CI 95%]: 1.434 [1.136-1.964], p: 0.036). While a significant positive correlation relationship was detected between total oxidative status and netrin-1 and LDL, a negative significant correlation relationship was detected between ADMA and eGFR. No significant difference was detected between the groups regarding serum endothelin-1, oxidative stress parameters, and L-arginine/methylarginine derivatives. As a result, this study showed that serum netrin-1 levels are high in asymptomatic diabetic PAH patients and can be a possible biomarker in the detection of asymptomatic PAH.

Keywords

Diabetes mellitus, oxidative stress, PAD, netrin-1, endothelin-1, ADMA

Ethical Statement

2014-322

Supporting Institution

Selçuk University Scientific Research Projects Coordination

Project Number

15102016.

Thanks

We thank the Selcuk University Scientific Research Projects Coordination Office for their unconditional support of this study.

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