Research Article
BibTex RIS Cite

Benzo(a)piren uygulaması durumunda kurkuminin sıçan sperm motilitesi ve morfolojisi üzerine olası koruyucu etkileri

Year 2020, Volume: 12 Issue: 2, 241 - 250, 01.06.2020
https://doi.org/10.21601/ortadogutipdergisi.723357

Abstract

Amaç: Çalışmada, sıçan sperm hareketliliği ve morfolojisi üzerinde benzo(a)pyrene (BaP) uygulamasının neden olduğu potansiyel hasarlar üzerinde kurkuminin olası koruyucu etkilerinin incelenmesi amaçlanmıştır.
Yöntemler: 36 adet Wistar albino sıçan altı gruba ayrılmıştır: Grup 1, Kontrol; Grup 2, Mısır Yağı (BaP) çözücüsü; Grup 3, DMSO (kurkumin çözücüsü); Grup 4, BaP; Grup 5, Kurkumin; Grup 6, BaP+Kurkumin. Altı haftalık oral uygulama sonrasında deneklerin sol kaudal epididimis ve duktus deferens dokuları Pure Sperm Wash (Nidacon) solüsyonuna alınarak (37°C) diseke edilerek sperm elde edildi. Spermler sayıldı ve boyandı. Manşetin perinükleer halkası ve nukleusun posterior parçası arası mesafe ölçülerek kaydedildi. Tüm veriler istatistiksel olarak analiz edildi.
Bulgular: BaP uygulaması sıçanlarda total sperm sayısı ile total hareketli sperm sayısında azalmaya; kuyruk ve akrozom anomalisi ile sitoplazmik droplet oranında ise artışa sebep olmuştur. Görülen anomalilerin kurkumin uygulaması ile beklenen oranlarda ve kontrol grubuna yaklaşacak şekilde düzelmediği ancak görülen anomali yüzdesinin azaldığı tespit edildi. Total sperm ve total hareketli sperm sayısının ise kurkumin uygulaması ile arttığı görüldü. Tüm veriler istatistiksel olarak da desteklendi.
Sonuç: Sonuç olarak kurkuminin bir antioksidan olarak, benzo(a)pyrene uygulamasının yol açtığı hasarlar üzerinde azalmaya yol açtığı ancak anomaliler üzerinde tamamen koruyucu olamadığı kanısına varıldı.

References

  • Banerjee B, Nandi P, Chakraborty S, Raha S, Sen PC, Jana K. Resveratrol ameliorates benzo(a)pyrene-induced testicular dysfunction and apoptosis: Involvement of p38 MAPK/ATF2/iNOS signaling. Journal of Nutritional Biochemistry 2016; 34: 17-29. (doi: 10.1016/j.jnutbio.2016.04.003).
  • Banerjee B, Chakraborty S, Ghosh D, Raha S, Sen PC, Jana K. Benzo(a)pyrene induced p53 mediated male germ cell apoptosis: Synergistic protective effects of curcumin and resveratrol. Frontiers in Pharmacology 2016; 7(245): 1-20. (doi: 10.3389/fphar.2016.00245).
  • Godschalk RW, Verhofstad N, Verheije M, Yauk CL, Linschooten J, Steeg H, Oostrom CT, Bnethem J, Schooten FJ. Effects of benzo[a]pyrene on Mouse germ cells: Heritable DNA mutation, testicular cell hypomethylation and their interaction with nucleotide excision repair. Toxicolocigal Research 2015; 4: 718-24. (doi: 10.1039/C4TX00114A).
  • Verhofstad N, Oostrom CT, Zwart E, Maas LM, Benthem J, Schooten FJ, Steeg H, Godschalk RWL. Evaluation of Benzo(a)pyrene-induced gene mutations in male germ cells. Toxicological Sciences 2011; 119(1): 218-23. (doi: 10.1093/toxsci/kfq325).
  • Kim KH, Jahan SA, Kabir E, Brown RJC. A review of airbone polycyclic hydrocarbons (PAHs) and their human health effects. Environment International 2013; 60: 71-80. (doi: 10.1016/j.envint.2013.07.019).
  • Borman SM, Christian PJ, Sipes IG, Hoyer PB. Ovotoxicity in female Fischer rats and B6 mice induced by low-dose exposure to three polycyclic aromatic hydrocarbons: comparison through calculation of an ovotoxic index. Toxicology and Applied Pharmacology 2000; 167: 191-8. (doi: 10.1006/taap.2000.9006).
  • Yauk CL, Quinn JS. Multilocus DNA fingerprinting reveals high rate of heritable genetic mutation in herring gulls nesting in an industrialized urban site. PNA1996; 93(22): 12137-41. (doi: 10.1073/pnas.93.22.12137).
  • Inyang F, Ramesh A, Kopsombut P, Niaz MS, Hood DB, Nyanda AM, Archibong AE. Disruption of testicular steroidogenesis and epididymal function by inhaled benzo(a)pyrene. Reproductive Toxicology 2003; 17: 527-37. (doi: 10.1016/S0890-6238(03)00071-6).
  • Moffat I, Chepelev NL, Labib S, Bourdon-Lacombe J, Kuo B, Buick JK, Lemieux F, Williams A, et al. Comparison of toxicogenomics and traditional approaches to inform mode of action and points of departure in human health risk assessment of benzo[a]pyrene in drinking water. Critical Reviews in Toxicology 2015; 8444: 1-43. (doi: 10.3109/10408444.2014.973934).
  • Mohamed EA, Song WH, Oh SA, Park YJ, You YA, Lee S, Choi JY, et al. The transgenerational impact of benzo(a)pyrene on murine male fertility. Human Reproduction 2010; 25(10): 2427-33. (doi: 10.1093/humrep/deq205).
  • Chung JY, Kim YJ, Kim JY, Lee SG, Park JE, Kim WR, Yoon YD, Yoo KS, Yoo YH, Kim JM. Benzo[a]pyrene reduces testosterone production in rat Leydig cells via direct disturbance of testicular steroidogenic machinery. Environmental Health Perspectives 2011; 119: 1569-74. (doi: 10.1289/ehp.1003391).
  • Sheweita SA, Al-Shora S, Hassan M. Effects of benzo[a]pyrene as an environmental pollutant and two natural antioxidants on biomarkers of reproductive dysfunction in male rats. Environmental Science and Pollution Research 2016; 23: 17226-35. (doi: 10.1007/s11356-016-6934-4).
  • Georgellis A, Parvinen M, Rydström J. Inhibition of stage-specific DNA synthesis in rat spermatogenic cells by polycyclic aromatic hydrocarbons. Chemico-Biological Interactions 1989; 72(1): 79-92.
  • Izadpanah M, Alizadeh R, Minaee MB, Heydari L, Babatunde A, Abbasi M. The effects of curcumin on sperm parameters and nitric oxide production in varicocelized rats. International Journal of Morphology 2015; 33(4): 1530-1535. (doi: 10.4067/S0717-95022015000400055).
  • Glombik K, Basta-Kaim A, Sikora-Polaczek M, Kubera M, Starowıcz G, Styrna J. Curcumin influences semen quality parameters and reverses the di(2-ethylhexyl)phthalate (DEHP)-induved testicular damage in mice. Pharmacological Reports 2014; 66: 782-7. (doi: 10.1016/j.pharep.2014.04.010).
  • Naz RK. The effect of curcumin on intracellular pH (pHi), membrane hyperpolarization and sperm motility. Journal of Reproduction & Infertility 2014; 15(2): 62-70.
  • Naz RK. Can curcumin provide an ideal contraceptive? Molecular Reproduction and Development 2011; 78(2):116-23. (doi: 10.1002/mrd.21276).
  • Momeni HR, Eskandari N. Curcumin inhibits the adverse effects of sodium arsenite in Mouse epididymal sperm. International Journal of Fertility & Sterility 2016; 10(2): 245-52.
  • Gupta SC, Patchva S, Aggarwa BB. Therapeutic roles of curcumin: Lessons learned from clinical trials. AAPS Journal 2013; 15(1): 195-218. (doi: 10.1208/s12248-012-9432-8).
  • Sahoo DK, Roy A, Chainy GB. Protective effects of vitamin E and curcumin on L-thyroxine-induced rat testicular oxidative stress. Chemico-Biological Interactions 2008; 176(2-3): 121-8. (doi: 10.1016/j.cbi.2008.07.009).
  • Oguzturk H, Ciftci O, Aydın M, Timurkaan N, Beytur A, Yılmaz F. Ameliorative effects of curcumin against acute cadmium toxicity on male reproductive system in rats. Andrologia 2012; 44(4): 243-9. (doi: 10.1111/j.1439-0272.2012.01273.x).
  • Reddy KP, Reddy PS. Testicular and epididymal toxicity induced by benzo(a)pyrene, alcohol, and their combination in Wistar rats. Toxicological Research 2016; 5: 420-33. (doi: 10.1039/c5tx00420a).
  • O’Brien JM, Beal MA, Yauk CL, Marchetti F. Benzo(a)pyrene is mutageniz in Mouse spermatogonial stem cells and dividing spermatogonia. Toxicological Sciences 2016; 152(2): 363-71. (doi: 10.1093/toxsci/kfw088).
  • Meier MJ, O’Brien JM, Beal MA, Allan, B Yauk CL, Marchetti F. In utero exposure to Benzo[a]pyrene increases mutation burden in the soma and sperm of adult mice. Environmental Health Perspectives 2017; 125(1): 82-8. (doi: 10.1289/EHP211).
  • Mochida K, Tres LL, Kierszenbaum AL. Isolation of the rat spermatid manchette and its perinuclear ring. Developmental Biology 1998; 200: 46-56. (doi: 10.1006/dbio.1998.8942).
  • O’Donnell L, Rhodes D, Smith SJ, Merriner DJ, Clark BJ, et al. An essential role for katanin p80 and microtubule severing in male gamete production. PLoS Genetics 2012; 8(5): 1-12. (doi: 10.1371/journal.pgen.1002698).
  • Khorsandi L, Mirhoseini M, Mohamadpour M, Orazizadeh M, Khaghani S. Effect of curcumin on dexamethasone-induced testicular toxicity in mice. Pharmaceutical Biology 2013; 51(2): 06-212. (doi: 10.3109/13880209.2012.716854).
  • Lu WP, Mei XT, Wang Y, Zheng YP, Xue Y, Xu DH. Zn (II)-curcumin protects against oxidative stress, deleterious changes in sperm parameters and histological alterations in a male mouse model of cyclophosphamide-induced reproductive damage. Environmental Toxicology and Pharmacology 2015; 39: 515-24. (doi: 10.1016/j.etap.2014.12.014).
  • Soleimanzadeh A, Saberivand A. Effect of curcumin on rat sperm morphology after the freze-thawing process. Veterinary Research Forum 2013; 4(3): 185-9.
  • Zhang L, Diao RY, Duan YG, Yi TH, Cai ZM. In vitro antioxidant effect of curcumin on human sperm quality in leucocytospermia. Andrologia 2017; 49(10): 1-6. (doi: 10.1111/and.12760).

The possible protective effects of curcumin in the case of benzo(a)pyrene administration on rat sperm motility and morphology

Year 2020, Volume: 12 Issue: 2, 241 - 250, 01.06.2020
https://doi.org/10.21601/ortadogutipdergisi.723357

Abstract

Background: In this study, our aim is to investigate the possible protective effects of curcumin on potential damage caused by benzo(a)pyrene (BaP) on rat sperm motility and morphology.
Methods: 36 Wistar Albino male rats were divided into six groups: Group 1, Control; Group 2, Corn oil (BaP dissolver); Group 3, DMSO (Curcumin dissolver); Group 4, BaP; Group 5, Curcumin; Group 6, BaP+Curcumin. By the end of the six week oral administration period, left caudal epididymis and ductus deferens tissues were placed in Pure Sperm Wash (Nidacon) solution (at 37°C) and dissected. Sperms were counted and stained. Measurements of the perinuclear ring of the manchette and the posterior portion of the nucleus were performed. All of the parameters were evaluated statistically.
Results: BaP administration caused a decrease in the number of total sperms and total motile sperms, while increasing abnormalities in tail, acrosome and cytoplasmic droplet particularly. Abnormalities did not approach the control group by curcumin administration in expected rates, but the percentage reduced. The number of total and motile sperms increased by curcumin. All parameters were statistically supported.
Conclusions: In conclusion, curcumin is one of the antioxidant that could be used potentially to inhibit the effects of Benzo(a)pyrene, but is ineffective in preventing all abnormalities.

References

  • Banerjee B, Nandi P, Chakraborty S, Raha S, Sen PC, Jana K. Resveratrol ameliorates benzo(a)pyrene-induced testicular dysfunction and apoptosis: Involvement of p38 MAPK/ATF2/iNOS signaling. Journal of Nutritional Biochemistry 2016; 34: 17-29. (doi: 10.1016/j.jnutbio.2016.04.003).
  • Banerjee B, Chakraborty S, Ghosh D, Raha S, Sen PC, Jana K. Benzo(a)pyrene induced p53 mediated male germ cell apoptosis: Synergistic protective effects of curcumin and resveratrol. Frontiers in Pharmacology 2016; 7(245): 1-20. (doi: 10.3389/fphar.2016.00245).
  • Godschalk RW, Verhofstad N, Verheije M, Yauk CL, Linschooten J, Steeg H, Oostrom CT, Bnethem J, Schooten FJ. Effects of benzo[a]pyrene on Mouse germ cells: Heritable DNA mutation, testicular cell hypomethylation and their interaction with nucleotide excision repair. Toxicolocigal Research 2015; 4: 718-24. (doi: 10.1039/C4TX00114A).
  • Verhofstad N, Oostrom CT, Zwart E, Maas LM, Benthem J, Schooten FJ, Steeg H, Godschalk RWL. Evaluation of Benzo(a)pyrene-induced gene mutations in male germ cells. Toxicological Sciences 2011; 119(1): 218-23. (doi: 10.1093/toxsci/kfq325).
  • Kim KH, Jahan SA, Kabir E, Brown RJC. A review of airbone polycyclic hydrocarbons (PAHs) and their human health effects. Environment International 2013; 60: 71-80. (doi: 10.1016/j.envint.2013.07.019).
  • Borman SM, Christian PJ, Sipes IG, Hoyer PB. Ovotoxicity in female Fischer rats and B6 mice induced by low-dose exposure to three polycyclic aromatic hydrocarbons: comparison through calculation of an ovotoxic index. Toxicology and Applied Pharmacology 2000; 167: 191-8. (doi: 10.1006/taap.2000.9006).
  • Yauk CL, Quinn JS. Multilocus DNA fingerprinting reveals high rate of heritable genetic mutation in herring gulls nesting in an industrialized urban site. PNA1996; 93(22): 12137-41. (doi: 10.1073/pnas.93.22.12137).
  • Inyang F, Ramesh A, Kopsombut P, Niaz MS, Hood DB, Nyanda AM, Archibong AE. Disruption of testicular steroidogenesis and epididymal function by inhaled benzo(a)pyrene. Reproductive Toxicology 2003; 17: 527-37. (doi: 10.1016/S0890-6238(03)00071-6).
  • Moffat I, Chepelev NL, Labib S, Bourdon-Lacombe J, Kuo B, Buick JK, Lemieux F, Williams A, et al. Comparison of toxicogenomics and traditional approaches to inform mode of action and points of departure in human health risk assessment of benzo[a]pyrene in drinking water. Critical Reviews in Toxicology 2015; 8444: 1-43. (doi: 10.3109/10408444.2014.973934).
  • Mohamed EA, Song WH, Oh SA, Park YJ, You YA, Lee S, Choi JY, et al. The transgenerational impact of benzo(a)pyrene on murine male fertility. Human Reproduction 2010; 25(10): 2427-33. (doi: 10.1093/humrep/deq205).
  • Chung JY, Kim YJ, Kim JY, Lee SG, Park JE, Kim WR, Yoon YD, Yoo KS, Yoo YH, Kim JM. Benzo[a]pyrene reduces testosterone production in rat Leydig cells via direct disturbance of testicular steroidogenic machinery. Environmental Health Perspectives 2011; 119: 1569-74. (doi: 10.1289/ehp.1003391).
  • Sheweita SA, Al-Shora S, Hassan M. Effects of benzo[a]pyrene as an environmental pollutant and two natural antioxidants on biomarkers of reproductive dysfunction in male rats. Environmental Science and Pollution Research 2016; 23: 17226-35. (doi: 10.1007/s11356-016-6934-4).
  • Georgellis A, Parvinen M, Rydström J. Inhibition of stage-specific DNA synthesis in rat spermatogenic cells by polycyclic aromatic hydrocarbons. Chemico-Biological Interactions 1989; 72(1): 79-92.
  • Izadpanah M, Alizadeh R, Minaee MB, Heydari L, Babatunde A, Abbasi M. The effects of curcumin on sperm parameters and nitric oxide production in varicocelized rats. International Journal of Morphology 2015; 33(4): 1530-1535. (doi: 10.4067/S0717-95022015000400055).
  • Glombik K, Basta-Kaim A, Sikora-Polaczek M, Kubera M, Starowıcz G, Styrna J. Curcumin influences semen quality parameters and reverses the di(2-ethylhexyl)phthalate (DEHP)-induved testicular damage in mice. Pharmacological Reports 2014; 66: 782-7. (doi: 10.1016/j.pharep.2014.04.010).
  • Naz RK. The effect of curcumin on intracellular pH (pHi), membrane hyperpolarization and sperm motility. Journal of Reproduction & Infertility 2014; 15(2): 62-70.
  • Naz RK. Can curcumin provide an ideal contraceptive? Molecular Reproduction and Development 2011; 78(2):116-23. (doi: 10.1002/mrd.21276).
  • Momeni HR, Eskandari N. Curcumin inhibits the adverse effects of sodium arsenite in Mouse epididymal sperm. International Journal of Fertility & Sterility 2016; 10(2): 245-52.
  • Gupta SC, Patchva S, Aggarwa BB. Therapeutic roles of curcumin: Lessons learned from clinical trials. AAPS Journal 2013; 15(1): 195-218. (doi: 10.1208/s12248-012-9432-8).
  • Sahoo DK, Roy A, Chainy GB. Protective effects of vitamin E and curcumin on L-thyroxine-induced rat testicular oxidative stress. Chemico-Biological Interactions 2008; 176(2-3): 121-8. (doi: 10.1016/j.cbi.2008.07.009).
  • Oguzturk H, Ciftci O, Aydın M, Timurkaan N, Beytur A, Yılmaz F. Ameliorative effects of curcumin against acute cadmium toxicity on male reproductive system in rats. Andrologia 2012; 44(4): 243-9. (doi: 10.1111/j.1439-0272.2012.01273.x).
  • Reddy KP, Reddy PS. Testicular and epididymal toxicity induced by benzo(a)pyrene, alcohol, and their combination in Wistar rats. Toxicological Research 2016; 5: 420-33. (doi: 10.1039/c5tx00420a).
  • O’Brien JM, Beal MA, Yauk CL, Marchetti F. Benzo(a)pyrene is mutageniz in Mouse spermatogonial stem cells and dividing spermatogonia. Toxicological Sciences 2016; 152(2): 363-71. (doi: 10.1093/toxsci/kfw088).
  • Meier MJ, O’Brien JM, Beal MA, Allan, B Yauk CL, Marchetti F. In utero exposure to Benzo[a]pyrene increases mutation burden in the soma and sperm of adult mice. Environmental Health Perspectives 2017; 125(1): 82-8. (doi: 10.1289/EHP211).
  • Mochida K, Tres LL, Kierszenbaum AL. Isolation of the rat spermatid manchette and its perinuclear ring. Developmental Biology 1998; 200: 46-56. (doi: 10.1006/dbio.1998.8942).
  • O’Donnell L, Rhodes D, Smith SJ, Merriner DJ, Clark BJ, et al. An essential role for katanin p80 and microtubule severing in male gamete production. PLoS Genetics 2012; 8(5): 1-12. (doi: 10.1371/journal.pgen.1002698).
  • Khorsandi L, Mirhoseini M, Mohamadpour M, Orazizadeh M, Khaghani S. Effect of curcumin on dexamethasone-induced testicular toxicity in mice. Pharmaceutical Biology 2013; 51(2): 06-212. (doi: 10.3109/13880209.2012.716854).
  • Lu WP, Mei XT, Wang Y, Zheng YP, Xue Y, Xu DH. Zn (II)-curcumin protects against oxidative stress, deleterious changes in sperm parameters and histological alterations in a male mouse model of cyclophosphamide-induced reproductive damage. Environmental Toxicology and Pharmacology 2015; 39: 515-24. (doi: 10.1016/j.etap.2014.12.014).
  • Soleimanzadeh A, Saberivand A. Effect of curcumin on rat sperm morphology after the freze-thawing process. Veterinary Research Forum 2013; 4(3): 185-9.
  • Zhang L, Diao RY, Duan YG, Yi TH, Cai ZM. In vitro antioxidant effect of curcumin on human sperm quality in leucocytospermia. Andrologia 2017; 49(10): 1-6. (doi: 10.1111/and.12760).
There are 30 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Original article
Authors

Cemile Merve Seymen 0000-0002-8945-3801

Iskender Kaplanoglu This is me 0000-0001-8065-5143

Gülnur Take 0000-0002-3661-3488

Gülce Naz Yazıcı 0000-0002-6989-997X

Deniz Erdoğan Erdoğan 0000-0002-0796-8095

Publication Date June 1, 2020
Published in Issue Year 2020 Volume: 12 Issue: 2

Cite

Vancouver Seymen CM, Kaplanoglu I, Take G, Yazıcı GN, Erdoğan DE. The possible protective effects of curcumin in the case of benzo(a)pyrene administration on rat sperm motility and morphology. otd. 12(2):241-50.

e-ISSN: 2548-0251

The content of this site is intended for health care professionals. All the published articles are distributed under the terms of

Creative Commons Attribution Licence,

which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.