The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells

Year 2023, Volume: 45 Issue: 2, 230 - 235, 15.03.2023

Evin Kocatürk , Eren Gunduz , Hüseyin Kayadibi , Hava Üsküdar Teke , İ. Özkan Alataş

https://doi.org/10.20515/otd.1210794

Abstract

The time of initiating stem cell collection is very important for successful transplantation and is determined according to peripheral blood CD34+ cell count analysed by flow cytometry. In this study, we aimed to find the role of new complete blood count parameters to determine the optimal time of stem cell collection. Eighty-six patients who underwent stem cell mobilization were included in the study. Peripheral blood CD34+ cells and complete blood count were analysed on the same day. Patients with peripheral blood CD 34+ cell counts ≤ 20/μL and > 20/μL were determined as Group 1 and Group 2, respectively. The difference of CBC parameters between 2 groups and the relationship of these parameters with peripheral blood CD34+ cell counts were evaluated. It was found that immature granulocytes ratio, nucleated red blood cells ratio and count were positively correlated with peripheral blood CD34+ cell count and these parameters were significantly different between two groups (P< 0.001, P= 0.011 and P= 0.012, respectively). The new index formulated by using immature granulocytes and nucleated red blood cells ratios was significantly different between two groups (P<0.001), and had the highest diagnostic accuracy to determine the time of stem cell collection (AUC= 0.766). Immature granulocytes and nucleated red blood cells can be used to determine the time of peripheral blood stem cell collection faster and cheaper compared to flow cytometry.

Keywords

Autologous transplantation, Stem cell mobilization, CD34+ cells, Immature granulocytes, Nucleated red blood cells

Otolog Nakil İçin Periferik Kan Hematopoietik Kök Hücre Toplamanın Optimal Zamanı, Olgunlaşmamış Granülositler ve Çekirdekli Kırmızı Kan Hücreleri Tarafından Tahmin Edilebilir

Abstract

Başarılı bir nakil için kök hücre toplamaya başlama zamanı çok önemlidir ve bu zaman akış sitometrisi ile analiz edilen periferik kan CD34+ hücre sayısına göre belirlenir. Bu çalışmada, kök hücre toplamanın optimal zamanını belirlemek için yeni tam kan sayımı parametrelerinin rolünü bulmayı amaçladık. Çalışmaya kök hücre mobilizasyonu uygulanan 86 hasta dahil edildi. Aynı gün periferik kan CD34+ hücreleri ve tam kan sayımı yapıldı. Periferik kan CD 34+ hücre sayısı ≤ 20/μL ve > 20/μL olan hastalar sırasıyla Grup 1 ve Grup 2 olarak belirlendi. İki grup arasındaki tam kan sayımı parametreleri farkı ve bu parametrelerin periferik kan CD34+ hücre sayısı ile ilişkisi değerlendirildi. İmmatür granülosit oranı, çekirdekli eritrosit oranı ve sayısının periferik kan CD34+ hücre sayısı ile pozitif korelasyon gösterdiği ve bu parametrelerin iki grup arasında anlamlı olarak farklı olduğu bulundu (sırasıyla P< 0.001, P= 0.011 ve P= 0.012). Olgunlaşmamış granülositler ve çekirdekli kırmızı kan hücreleri oranları kullanılarak formüle edilen yeni indeks, iki grup arasında önemli ölçüde farklıydı (P<0.001) ve kök hücre toplama zamanını belirlemek için en yüksek tanı doğruluğuna sahipti (AUC= 0.766). İmmatür granülositler ve çekirdekli kırmızı kan hücreleri, akış sitometrisine göre periferik kan kök hücre toplama zamanını daha hızlı ve daha ucuza belirlemek için kullanılabilir.

Keywords

Autologous transplantation, Stem cell mobilization, CD34+ cells, Immature granulocytes, Nucleated red blood cells

References

• 1. Bazinet A, Popradi G. A general practitioner's guide to hematopoietic stem-cell transplantation. Curr Oncol. 2019;26:187-91.
• 2. Sakashita AM, Kondo AT, Yokoyama APH, et al. The impact of preapheresis white blood cell count on autologous peripheral blood stem cell collection efficiency and HSC infusion side effect rate. J Clin Apher. 2018;33:331-41.
• 3. Pusic I, Jiang SY, Landua S, et al. Impact of mobilization and remobilization strategies on achieving sufficient stem cell yields for autologous transplantation. Biol Blood Marrow Transplant. 2008;14:1045-56.
• 4. Steussy BW, Capper M, Krasowski MD, et al. Algorithms utilizing peripheral blood hematopoietic progenitor cell counts in lieu of some CD34+ cell counts predict successful peripheral blood stem cell collections with substantial time and cost savings. ISBT Sci Ser. 2016;11:153-62.
• 5. Sutherland DR, Anderson L, Keeney M, et al. The ISHAGE guidelines for CD34+ cell determination by flow cytometry. International Society of Hematotherapy and Graft Engineering. J Hematother. 1996;5:213-26.
• 6. Tanyeli A, Aykut G, Demirel AO, et al. Hematopoietic Stem Cell Transplantation and its History. Archives Medical Review Journal. 2014;23:1-7.
• 7. Swift MN, Taketa ST, Bond VP. Efficacy of hematopoietic protective procedure in rats x-irradiated with intestine shielded. Radiat Res. 1956;4:186-92.
• 8. Jamal A, Khan MT, Parveen S, et al. Peripheral Blood Stem Cell Harvest HPC Count Is an Effective Surrogate Marker for CD34+ Cell Count in Allogeneic Stem Cell Transplant Setting. Transl Oncol. 2020;13:100788.
• 9. Gutensohn K, Magens M, Kruger W, et al Comparison of flow cytometry vs. a hematology cell analyser-based method to guide the optimal time-point for peripheral blood stem cell apheresis. Sang. 2006;90:53-8.
• 10. Lefrere F, Zohar S, Beaudier S, et al. Evaluation of an algorithm based on peripheral blood hematopoietic progenitor cell and CD34+ cell concentrations to optimize peripheral blood progenitor cell collection by apheresis. Transfusion. 2007;47:1851-7.
• 11. Letestu R, Marzac C, Audat F, et al. Use of hematopoietic progenitor cell count on the Sysmex XE-2100 for peripheral blood stem cell harvest monitoring. Leuk Lymphoma. 2007;48:89–96.
• 12. Padmanabhan A, Reich-Slotky R, Jhang JS, et al. Use of the hematopoietic progenitor cell parameter in optimizing timing of peripheral blood stem cell harvest. Vox Sang. 2009;97:153-9.
• 13. Peerschke EI, Moung C, Pessin MS, et al. Evaluation of new automated hematopoietic progenitor cell analysis in the clinical management of peripheral blood stem cell collections. Transfusion. 2015;55:2001-9.
• 14. Steussy BW, Capper M, Krasowski MD, et al. Algorithms utilizing peripheral blood hematopoietic progenitor cell counts in lieu of some CD34 + cell counts predict successful peripheral blood stem cell collections with substantial time and cost savings. ISBT Sci Ser. 2016;11:153-62.
• 15. May JE, Marques MB, Reddy VVB, et al. Three neglected numbers in the CBC: The RDW, MPV, and NRBC count. Cleve Clin J Med. 2019;86:167-72.
• 16. Chabot-Richards DS, George TI. White blood cell counts: reference methodology. Clin Lab Med. 2015;35:11-24.
• 17. Sysmex parameters. https://www.sysmex-europe.com/academy/knowledge-centre/sysmex-parameters.html. Accessed January 3, 2022.