Desfluranın Aynı Konsantrasyonda Fakat Farklı Akımlarda Kullanılması Oksidatif Stresi Değiştiştirir mi? Hayvan Deneyi

Year 2019, Volume: 4 Issue: 4, 492 - 505, 31.12.2019

Çiğdem Ünal Kantekın , Müge Çakırca , Ferda Yaman , Ayşe Yeşim Göçmen

https://doi.org/10.26453/otjhs.490617

Abstract

İnhalasyon
anesteziklerinin oksidatif stres ve serbest radikallerin oluşumunda etkili
olduğu bilinmektedir. Bu çalışmanın amacı, desfluranın bu etkilerini araştırmak
ve farklı akımlarda oluşan 
değişiklikleri saptamaktır. Fareler üç gruba ayrıldı (10 fare / grup).
Birinci grup kontrol grubu idi. İkinci grupta, ketamin ile sedasyon sağlanan
farelere bir cam kutuda  % 100 oksijen
içerisinde % 6 konsantrasyonda,  20
dakika boyunca, 8 lt / dk akış hızında desfluran verildi. Üçüncü grupta bir cam
kutu içerisinde  ketamin ile sedasyon
sağlanmış farelere, % 100 oksijen içerisinde % 6 konsantrasyonda, 20 dakika
boyunca, 2 lt / dk akış hızında desfluran verildi. Biyokimyasal parametreler
ölçüldü. Serum, beyin, karaciğer dokusu MDA ve SOD düzeyleri yüksek akım grubunda
kontrol ve düşük akım grubundan anlamlı olarak daha yüksek bulundu. Her iki
gruba göre serum GPx düzeyleri yüksek akım grubunda anlamlı olarak düşüktü.
Serum, karaciğer ve beyin dokusunda ADMA düzeyleri yüksek akım grubunda anlamlı
olarak yüksek bulundu. Her üç grup arasında serum 8-OHdG seviyelerinde anlamlı
fark yoktu. Beyin ve karaciğer dokusu 8-OHDG düzeyleri her iki grupta kontrol
grubuna göre daha yüksekti.  Desfluran
anestezi modelinde, desfluranın aynı konsantrasyonlarda  düşük akımda kullanıldığında oksidatif stresi
azalttığı, yüksek akımda kullanıldığında ise oksidatif stresi arttırdığı
görülmüştür.

Keywords

Desfluran, oksidatif stres, düşük akım, yüksek akım

Does the Application of Desflurane at The Same Concentration by Different Flows Change the Oxidative Stress? Animal Experiment

Abstract

It is known that inhalation
anesthetics are effective in the formation of oxidative stress and free
radicals. The purpose of this study is to investigate these effects of
desflurane and to detect changes in different flows. The rats were divided into three groups (10 mice/group). The first
group was the control group. In the second group, mice were sedated with
ketamine and given desflurane at a concentration of 6% in a 100% oxygen flow at
a flow rate of 8 lt / min in a glass box for 20 minutes. The third group was
comprised of ketamine-sedated mice that were given desflurane at a
concentration of 6% in a 100% oxygen flow at a flow rate of 2 lt/min in a glass
box for 20 minutes. Biochemical parameters were measured using commercial kits.
Serum, brain, liver tissue MDA and SOD levels were significantly higher in the
high flow group than in the control and low flow group. Serum GPx levels were
significantly lower in the high flow group compared to both groups. ADMA levels
in the serum, liver, and brain tissue were significantly higher in the high
flow group. There was no significant difference in serum 8-OHdG levels between
all three groups. Brain and liver tissue 8-OHDG levels were higher in both
groups than control group. It has been observed that the use of low flow fresh
gas even in the same concentrations of desflurane anesthesia models decreases
oxidative stress whereas the use of high flow increases oxidative stress. 

Keywords

Desflurane, oxidative stress, low flow, high flow

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