Geleneksel Kemoterapötik Kombinasyonların (FOLFOX, FOLFIRI) Kolon Kanseri Hücreleri Üzerindeki Büyüme-Engelleyici Etkileri: Bir in vitro Çalışma

Year 2024, Volume: 9 Issue: 4, 349 - 356, 25.12.2024

Aslı Yıldırım Kocaman , Seçil Erden Tayhan

https://doi.org/10.26453/otjhs.1538781

Abstract

Amaç: Yaygın olarak kullanılan kemoterapötik kombinasyonlar olan folinik asit, 5-florourasil ve oksaliplatin (FOLFOX) ile folinik asit, 5-florourasil ve irinotekanın (FOLFIRI) kolon kanseri hücreleri (HT29 ve CaCo-2 hücreleri) üzerindeki in vitro büyümeyi inhibe edici etkilerini karşılaştırmak.
Materyal ve Metot: FOLFOX ve FOLFIRI kombinasyonlarının farklı konsantrasyonlarıyla tedavi edilen HT29 ve CaCo-2 hücrelerinin canlılığı MTT (3-(4,5-dimetiltiazolil-2)-2,5-difeniltetrazolium bromür) testi kullanılarak değerlendirildi.
Bulgular: FOLFOX ve FOLFIRI kombinasyonları kolon kanseri hücre hatları üzerinde farklı etkiler gösterdi, HT29 hücreleri duyarlılık gösterirken, CaCo-2 hücreleri bu tedavilere direnç gösterdi.
Sonuç: Bu ön çalışmanın sonuçları, kolon kanseri için etkili ve hedefli klinik tedavi stratejilerinin geliştirilmesine katkıda bulunacaktır.

Keywords

Antiproliferatif etki, FOLFIRI, FOLFOX, ilaç kombinasyonu, kolon kanseri

Ethical Statement

This research utilized commercially available cell lines. As such, no ethics committee approval was necessary for this study. The research adhered to international declarations and guidelines.

Growth-Inhibitory Effects of Traditional Chemotherapeutic Combinations (FOLFOX, FOLFIRI) on Colon Cancer Cells: An in vitro Study

Abstract

Objective: To compare the in vitro growth-inhibitory effects of the commonly used chemotherapeutic combinations folinic acid, 5-fluorouracil, and oxaliplatin (FOLFOX) and folinic acid, 5-fluorouracil, and irinotecan (FOLFIRI) on colon cancer cells (HT29 and CaCo-2 cells).
Materials and Methods: The viability of HT29 and CaCo-2 cells treated with different concentrations of the FOLFOX and FOLFIRI combinations was evaluated using the MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) assay.
Results: FOLFOX and FOLFIRI combinations exhibited varying effects on the colon cancer cell lines, with HT29 cells showing sensitivity, while CaCo-2 cells demonstrated resistance to these treatments.
Conclusions: The results of this preliminary study will contribute to the development of effective and targeted clinical treatment strategies for colon cancer.

Keywords

Colon cancer, drug combination, FOLFOX, FOLFIRI, growth-inhibitory effect

Ethical Statement

This research utilized commercially available cell lines. As such, no ethics committee approval was necessary for this study. The research adhered to international declarations and guidelines.

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