Effect of Tryptanthrin Compound on Lipop olysa cchar ide-I nduce d Inflammation Model in L929 Cell Line

Year 2023, Volume: 3 Issue: 3, 54 - 58, 31.07.2023

Mehmet Yılmaz Zerrin Kutlu

https://doi.org/10.5152/Pharmata.2023.23010

Abstract

Objective: Our aim is to evaluate the effects of the tryptanthrin compound, with antimicrobial
and anti-inflammatory effects, on the lipopolysaccharide inflammation model created in the
L929 cell line.
Methods: L929 cell lines were placed in a 5% CO2 incubator at 37°C, using standard cell culture
procedures, and lipopolysaccharide was applied with Prn pure substance with a final concentration
of 2 μL and 1 μL after 1 hour. Following the lipopolysaccharide application, the (3- [4,5- dimethylthiazol-
2-yl] -2,5-diphenyl tetrazolium bromide protocol, a colorimetric method, was applied to
determine cell viability at the end of the required 24th, 48th, and 72nd hours incubation times.
Results: IC50 values were calculated as 0.3 μg/mL for tryptanthrin and 1 μg/mL for lipopolysaccharide
. Application of lipopolysaccharide to L929 cell lines caused a significant decrease in cellular
index depending on time. Also, in the tryptanthrin + lipopolysaccharide groups, it was found
that the decreased cell index significantly increased even closer to the control compared to the
lipopolysaccharide applied group. It was found that the inflammation and cell damage caused by
lipopolysaccharide applied to the L929 cell line improved after tryptanthrin application.
Conclusion: When we look at the results of our study as a whole, it was determined that the
viability rates of the cells were above 85% as a result of the tryptanthrin application and it was significantly
above the 70% viable cell ratio recommended by ISO 10993-5: 2009 Dın en ISO (2009)
for cytotoxicity.

Keywords

Inflammation, L929, LPS, MTT, Tryptanthrin

Ethical Statement

There is no 'Ethics committee approval because this is a cell culture study.

References

• 1. Popov A, Klimovich A, Styshova O, et al. Design, synthesis and biomedical evaluation of mostotrin, a new water soluble tryptanthrin derivative. Int J Mol Med. 2020;46(4):1335-1346. [CrossRef]
• 2. Moeed A, Michael M, Emma GM. Fibroblast activation and inflammation in frozen shoulder. Plus One. 2019:1-16.
• 3. Chen L, Deng H, Cui H, et al. Inflammatory responses and infla mmati on-as socia ted diseases in organs. Oncotarget. 2018;9(6):7204-7218. [CrossRef]
• 4. Kaminska B. MAPK signalling pathways as molecular targets for antiinflammatory therapy--from molecular mechanisms to therapeutic benefits. Biochim Biophys Acta. 2005;1754(1-2):253-262. [CrossRef]
• 5. Netea MG, Balkwill F, Chonchol M, et al. A guiding map for inflammation. Nat Immunol. 2017;18(8):826-831. [CrossRef]
• 6. Xu J, Zhao Y, Aisa HA. Anti-inflammatory effect of pomegranate flower in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Pharmaceutıcal Bıology. 2017;55(1):2095-2101. [CrossRef]
• 7. Shao J, Li Y, Wang Z. A novel naphthalimide derivative, exhibited antiinflammatory effects via targeted-inhibiting TAK1 following downregulation of ERK1/2- and p38 MAPK-mediated activation of NF-kB in LPS-stimulated RAW264.7 macrophages. Int Immunupharmacol. 2013;17:216-228.
• 8. Zhang S, Ma J, Sheng L, et al. Total Coumarins from Hydrangea paniculata show renal protective effects in lipop olysa cchar ide-i nduce d acute kidney injury via anti-inflammatory and antioxidant activities. Front Pharmacol. 2017;8:872. [CrossRef]
• 9. Vysakh A, Prasad G, Kuriakose J. Rotula aquatica Lour attenuates secretion of proinflammatory mediators and cytokines in lipop olysa cchar ide-i nduce d inflammatory responses in murine RAW 264.7 macrophages. Inflammopharmacology. 2018;26:29-38.
• 10. Guha M, Mackman N. LPS induction of gene expression in human monocytes. Cell Signal. 2001;13(2):85-94. [CrossRef]
• 11. Bistrian B. Systemic response to inflammation. Nutr Rev. 2007;65(12 Pt 2):S170-S172. [CrossRef]
• 12. Mogensen TH. Pathogen recognition and inflammatory signaling in innate immune defenses. Clin Microbiol Rev. 2009;22(2):240-273. [CrossRef]
• 13. Neumann U, Louis S, Gille A, et al. Anti-inflammatory effects of Phaeodactylum tricornutum extracts on human blood mononuclear cells and murine macrophages. J Appl Phycol. 2018;30(5):2837-2846. [CrossRef]
• 14. Mustafa YF, Najem MA, Tawffiq ZS. Coumarins from creston apple seeds: isolation, chemical modification, and cytotoxicity study. J Appl Pharm Sci. 2018;8(08):49-56.
• 15. Kasumbwe K., Kabange N., Venugopala N. 2017 Synthetic Mono/dihalogenated Coumarin Derivatives and Their Anticancer Properties. Anti-Cancer Agents in Medicinal Chemistry, 17(2),276-85.