Preparation of Chitosan and Glycol Chitosan Coated Magnetic Nanoparticles Loaded with Carboplatin as Anticancer Drug

Year 2019, Volume: 22 Issue: 4, 1017 - 1022, 01.12.2019

Ceren Atila Dinçer , Ali Mert Erdek Ayşe Karakeçili , Nuray Yıldız

https://doi.org/10.2339/politeknik.501694

Cited By: 2

Abstract

Surface modified Fe3O4 nanoparticles (Fe3O4-OA) with an average diameter of 10 nm were synthesized, coated by chitosan (CS) and glycol chitosan (GCS), thus magnetic polymer nanocomposites were obtained. The magnetic nanostructures were analyzed by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), X-Ray diffraction (XRD) and vibrating sample magnetometer (VSM). All magnetic structures synthesized in this study exhibited superparamagnetic properties. Loading carboplatin (CPt) as anticancer drug to Fe3O4-OA-GCS nanocomposites were carried out with 13.17 % drug content and 38 % encapsulation efficiency. The cytotoxicity studies were occured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay on L929 mouse fibroblasts and MCF-7 human breast cancer cells. Fe3O4-OA-GCS-CPt nanocomposites showed higher cytotoxicity than free CPt on the MCF-7 cells at 50 µg/ml drug concentrations during 72 h.

Keywords

Fe3O4-OA nanoparticles, chitosan / glycol chitosan coating, carboplatin, anticancer drug loading, drug release

References

• Zhang, L., He, R. and Gu, H. C., “Oleic acid coating on the monodisperse magnetite nanoparticles”, Applied Surface Science, 253: 2611-2617, (2006).
• Lan, Q., Liu, C., Yang, F., Liu, S., Xu, J. and Sun, D., “Synthesis of bilayer oleic acid-coated Fe3O4 nanoparticles and their application in pH-responsive Pickering emulsions”, Journal of Colloid and Interface Science, 310: 260-269, (2007).
• Bagherpour, A. R., Kashanian, F., Ebrahimi, S. S. and Habibi-Rezaei, M., “L-arginine modified magnetic nanoparticles: green synthesis and characterization”, Nanotechnology, 29: 075706, (2018).
• Gupta, A. K. and Gupta, M., “Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications”, Biomaterials, 26: 3995-4021, (2005).
• Mahdavi, M., Ahmad, M. B., Haron, M. J., Namvar, F., Nadi, B., Rahman, M. Z. A. and Amin, J., “Synthesis, surface modification and characterisation of biocompatible magnetic iron oxide nanoparticles for biomedical applications”, Molecules, 18: 7533-7548, (2013).
• Agnihotri, S. A., Mallikarjuna, N. N. and Aminabhavi, T. M., “Recent advances on chitosan-based micro-and nanoparticles in drug delivery”, Journal of Controlled Release, 100: 5-28, (2004).
• Pillai, C. K. S., Paul, W. and Sharma, C. P., “Chitin and chitosan polymers: Chemistry, solubility and fiber formation”, Progress in Polymer Science, 34: 641-678, (2009).
• Park, J. H., Saravanakumar, G., Kim, K. and Kwon, I. C., “Targeted delivery of low molecular drugs using chitosan and its derivatives”, Advanced Drug Delivery Reviews, 62: 28-41, (2010).
• Li, P., Wang, Y., Peng, Z., She, F. and Kong, L., “Development of chitosan nanoparticles as drug delivery systems for 5-fluorouracil and leucovorin blends”, Carbohydrate Polymers, 85: 698-704, (2011).
• Trapani, A., Sitterberg, J., Bakowsky, U. and Kissel, T., “The potential of glycol chitosan nanoparticles as carrier for low water soluble drugs”, International Journal of Pharmaceutics, 375: 97-106, (2009).
• Nanjwade, B.K., Singh, J., Parikh, K.A. and Manvi, F.V., “Preparation and evaluation of carboplatin biodegradable polymeric nanoparticles”, International Journal of Pharmaceutics, 385: 176-180, (2010).
• Jose, S., Juna, B. C., Cinu, T. A., Jyoti, H., and Aleykutty, N. A., “Carboplatin loaded Surface modified PLGA nanoparticles: Optimization, characterization, and in vivo brain targeting studies”, Colloids and Surfaces B: Biointerfaces, 142: 307-314, (2016).
• Yang, H., Tang, C. and Yin, C., “Estrone-modified pH-sensitive glycol chitosan nanoparticles for drug delivery in breast cancer”, Acta Biomaterialia, 73: 400-411, (2018).
• Zamora-Mora, V., Fernández-Gutiérrez, M., González-Gómez, Á., Sanz, B., San Román, J., Goya, G.F., Hernández, R. and Mijangos, C., “Chitosan nanoparticles for combined drug delivery and magnetic hyperthermia: From preparation to in vitro studies”, Carbohydrate Polymers, 157: 361-370, (2017).
• Zhong, S., Zhang, H., Liu, Y., Wang, G., Shi, C., Li, Z., Feng, Y. and Cui, X., “Folic acid functionalized reduction-responsive magnetic chitosan nanocapsules for targeted delivery and triggered release of drugs”, Carbohydrate Polymers, 168: 282-289, (2017).
• Atila Dinçer, C., Yıldız, N., Aydoğan, N., and Çalımlı, A., “A comparative study of Fe3O4 nanoparticles modified with different silane compounds”, Applied Surface Science, 318; 297-304, (2014).
• Tansık, G., Yakar, A. and Gündüz, U., “Tailoring magnetic PLGA nanoparticles suitable for doxorubicin delivery”, Journal of Nanoparticle Research, 16: 2171, (2014).
• Tığlı Aydın, R. S., and Pulat M., “5-Fluorouracil encapsulated chitosan nanoparticles for pH-stimulated drug delivery: evaluation of controlled release kinetics”, Journal of Nanomaterials, 42: 1-10, (2012).
• Gökçe Y., Cengiz B., Yıldız N., Çalımlı A., Aktaş Z., “Ultrasonication of chitosan nanoparticle suspension: Influence on particle size”, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 462: 75-81, (2014).
• Qin, H., Wang, C. M., Dong, Q. Q., Zhang, L., Zhang, X., Ma, Z. Y. and Han, Q. R., “Preparation and characterization of magnetic Fe3O4–chitosan nanoparticles loaded with isoniazid”, Journal of Magnetism and Magnetic Materials, 381: 120-126, (2015).
• Atila Dinçer, C., Yıldız N., Karakeçili A., Aydoğan N., and Çalımlı A., “Synthesis and characterization of Fe3O4-MPTMS-PLGA nanocomposites for anticancer drug loading and release studies”, Artificial Cells, Nanomedicine and Biotechnology, 45: 1408-1414, (2017).
• Khan, M. A., Zafaryab, M., Mehdi, S. H., Quadri, J. and Rizvi, M. M. A., “Characterization and carboplatin loaded chitosan nanoparticles for the chemotherapy against breast cancer in vitro studies”, International Journal of Biological Macromolecules, 97: 115-122, (2017).
• Gu, S., Onishi, J., Kobayashi, Y., Nagao, D. and Konno M., “Preparation and colloidal stability of monodisperse magnetic polymer particles”, Journal of Colloid and Interface Science, 289: 419-26, (2005).
• Wu, S., Sun, A., Zhai, F., Wang, J., Xu, W., Zhang, Q. and Volinsky, A. A., “Fe3O4 magnetic nanoparticles synthesis from tailings by ultrasonic chemical co-precipitation”, Materials Letters, 65: 1882–1884, (2011).
• Haldorai, Y., Pham, Q. L., and Shim, J. J., “Supercritical fluid mediated synthesis of poly (2-hydroxyethyl methacrylate)/Fe3O4 hybrid nanocomposite”, Materials Science and Engineering: B, 176: 773-778, (2011).
• Qu, J., Liu, G., Wang, Y. and Hong, R., “Preparation of Fe3O4–chitosan nanoparticles used for hyperthermia”, Advanced Powder Technology, 21: 461-467, (2010).
• Inbaraj, B. S., Tsai, T. Y. and Chen, B. H., “Synthesis, characterization and antibacterial activity of superparamagnetic nanoparticles modified with glycol chitosan”, Science and Technology of Advanced Materials, 13: 015002, (2012).