Düşük Doz Doksisiklin ve Kişniş ile Tedavi Edilen Deneysel Periodontitisde Serum Antioksidan Seviyelerinin ve İnflamatuar Hücre Yoğunluğunun Karşılaştırılması

Year 2022, Volume: 12 Issue: 3, 445 - 452, 20.09.2022

Şeyma Bozkurt Doğan , Umut Ballı , Figen Öngöz Dede , Kanat Gülle , Bahattin Avcı , Hatice Çölgeçen , Meryem Akpolat Ferah , Mazlum Bülent Kurtiş

https://doi.org/10.33631/sabd.1054420

Abstract

Amaç: Bu çalışmanın amacı deneysel olarak periodontitis oluşturulmuş ratlarda sistemik olarak uygulanmış düşük doz doksisiklinin (LDD) ve kişniş bitkisinin (CSL) serumdaki antioksidan enzim seviyeleri ve inflamatuar hücre miktarı üzerine olan etkilerini değerlendirmektir.
Gereç ve Yöntemler: Çalışmanın başlangıncında 40 adet Wistar Albino erkek rat rastgele beş eşit gruba ayrıldı. Grup 1: periodontal sağlıklı; grup 2: periodontitis; grup 3: periodontitis + CSL (32 mg/kg); grup 4: periodontitis +CSL (200mg/kg); grup 5: periodontitis + doksisiklin hidroklorid (6 mg/kg). Elde edilen serum örneklerinde superoksit dismutanz (SOD), glutatyon peroksidaz (GSH-Px), katalaz (CAT) seviyeleri ELISA metodu uygulanarak analiz edildi. İnflamatuar hücre yoğunluğu histopatolojik olarak değerlendirildi.
Bulgular: Serumdaki SOD seviyesi diğer gruplar ile karşılaştırıldığında istatistiksel olarak en düşük grup 1 ve istatistiksel olarak en yüksek ise grup 2 de bulundu. SOD seviyesi grup 1 ve 2 nin diğer gruplarla olan ikili karşılaştırmalarında istatistiksel olarak önemli bulurken, grup 3, 4 ve 5 arasında istatistiksel olarak önemli bir fark göstermedi. CAT seviyesi diğer gruplar ile karşılaştırıldığında istatistiksel olarak en düşük grup 2'de bulunurken, grup 1, 3, 4 ve 5 arasında önemli bir farklılık göstermedi. GSH-Px seviyesi gruplar arasında istatistiksel olarak önemli farklılık göstermedi. İnflamatuar hücre yoğunluğu grup 2 ve 4 de grup 1’e göre istatistiksel olarak önemli derecede yüksek bulundu. Grup 1, 3 ve 5 arasında inflamatuar hücre yoğunluğu açısından istatistiksel olarak önemli farklılık rapor edilmedi.
Sonuç: Serum SOD, CAT ve inflamatuar hücre yoğunluğu bakımından CSL ve LDD uygulama grupları arasında farklılık gözlenmedi. Bu yüzden, periodontitis tedavisinde farklı CSL dozajlarının uygulandığı çalışmaların yapılması gerektiğini düşünmekteyiz.

Keywords

Periodontitis, kişniş, antioksidan, düşük doz doksisiklin, serum

Supporting Institution

TÜBİTAK

Project Number

11S889

Comparison Effects of Serum Antioxidants Levels and Intensity of Inflammatory Cells in Experimental Periodontitis Treated with Low-Dose Doxycycline and Coriandrum Sativum L

Abstract

Aim: The aim of this study was to evaluate the effects of systemically-administered Coriandrum sativum L (CSL) and low dose doxycycline (LDD) on serum levels of antioxidant enzymes and intensity of inflammatory cells in rats with experimental periodontitis.
Material and Methods: Forty adult male Wistar Albino rats were divided randomly into 5 groups as follows: group 1: periodontally healthy (control); group 2: periodontitis; group 3: periodontitis+CSL (32mg/kg); group 4: periodontitis+CSL (200mg/kg); group 5: periodontitis+LDD (6 mg/kg). Serum gingival superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) levels were evaluated by ELISA. The intensity of inflammatory cells were evaluated by histopathologically.
Results: SOD levels were statistically lowest in group 1 and statistically were highest in group 2 than those of other groups. There was a statistical difference in SOD levels in paired comparisons of groups 1 and 2 with other groups. Its level did not have statistically significant among groups 3, 4 and 5. CAT levels were statistically lowest in group 2 than those of other groups, and no differences were reported among groups 1, 3, 4, and 5. GSH-Px levels did not have statistically significant among groups. Inflammatory cell infiltration was found to be statistically higher in groups 2 and 4 compared to group 1, and no statistical significance was reported among groups 1, 3, and 5.
Conclusion: CSL and LDD application groups did not show differences in terms of serum SOD, serum CAT, and intensity of inflammatory cells. Therefore, we suggest that the different dosages of CSL should be examined in the treatment of periodontitis.

Keywords

Periodontitis, Coriander Sativum L, Low Dose doxcycline, serum, antioxidant

Project Number

11S889

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