Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats

Year 2018, Volume: 22 Issue: 6, 1674 - 1682, 01.12.2018

Fatma Özdemir , Seza Arslan

https://doi.org/10.16984/saufenbilder.395016

Cited By: 1

Abstract

The genus Bacillus
is frequently found in soil, water and food. Bacillus cereus and Bacillus
anthracis are the main pathogens causing foodborne diseases and serious
infections in humans. A total of 52 Bacillus
spp. from meat samples was tested for determination of biofilm production, antimicrobial
resistance pattern and beta-lactamase activity. The 24 (46.1%) Bacillus isolates were found to be for
biofilm production. Of the 24 (46.1%) biofilm producer Bacillus isolates, 13 (25%), 6 (11.5%) and 5 (9.6%) were considered
as strong, moderate and weak biofilm producer, respectively. The most common species
for the production of biofilm was Bacillus
thuringiensis (80%). Antimicrobial disk susceptibility tests of Bacillus spp. revealed high resistance
to ampicillin (84.6%) followed by penicillin (75%), cefepime (34.6%), and
cefoxitin (26.9%). A multidrug resistance to at least 3 or more antimicrobials
was observed in the 25 isolates (48.1%). All Bacillus spp. were sensitive to vancomycin, gentamicin, amikacin, ciprofloxacin,
and imipenem. The susceptibility rate to streptomycin, chloramphenicol, and
trimethoprim-sulphamethoxazole was 94.2%. Among the isolates, the 6 (11.5%) isolates
were found to be sensitive to all antimicrobial agents tested. Besides, only
one isolate from meat was found to be positive for beta-lactamase test. The
existence of biofilm production as a virulence factor and of multidrug
resistance in bacteria isolated from food should not be underestimated in terms
of food safety, public health and economic concerns.

Keywords

Bacillus spp., biofilm production, antimicrobial resistance, beta-lactamase

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