Antimicrobial and antibiofilm activity of green synthesized silver nanoparticles by using aqueous leaf extract of Thymus serpyllum

Year 2019, Volume: 23 Issue: 3, 333 - 339, 01.06.2019

Fatih Erci , Emrah Torlak

https://doi.org/10.16984/saufenbilder.445146

Cited By: 14

Abstract

Recently, metal nanoparticles have attracted the attention of researchers due to their unique properties when compared with bulk materials and have become used in many fields of application. In this study, green synthesis of Ag nanoparticles (AgNPs) was investigated by using aqueous extract of Thymus serpyllum leaves. In addition, antimicrobial and antibiofilm activities of the synthesized AgNPs were evaluated in this study. Further, UV-vis spectrophotometer, FTIR, DLS, SEM with EDX and TEM were used for characterization of the green synthesized AgNPs. The UV-vis spectrum of the synthesized AgNPs had a maximum peak at 467 nm. Also, TEM analysis indicated spherical particles with an average size of 25.2 nm. The synthesized AgNPs have higher stability (zeta potential: -29.5 mV). The antimicrobial activity of the green synthesized AgNPs was investigated on both Gram-positive and Gram-negative bacteria, such as Bacillus cereus, Staphylococcus aureus, Escherichia coli and Salmonella typhimurium using agar well diffusion assay. According to the results of the study, Gram-positive bacteria showed larger inhibition zones compared to Gram-negative bacteria. Finally, the AgNPs were explored for the inhibition of S. aureus biofilms. AgNPs at 100 μg/mL concentration showed a high inhibition value of about 73% for S. aureus biofilm formation. So, it is concluded that the synthesized AgNPs might be potentially used in many applications due to their antimicrobial and antibiofilm properties.

Keywords

Green synthesis, silver nanoparticle, antimicrobial

References

• A. Nel, T. Xia, L. Mädler, and N. Li, "Toxic potential of materials at the nanolevel," science, vol. 311, no. 5761, pp. 622-627, 2006.
• K. N. Thakkar, S. S. Mhatre, and R. Y. Parikh, "Biological synthesis of metallic nanoparticles," Nanomedicine: Nanotechnology, Biology and Medicine, vol. 6, no. 2, pp. 257-262, 2010.
• A. Albanese, P. S. Tang, and W. C. Chan, "The effect of nanoparticle size, shape, and surface chemistry on biological systems," Annual review of biomedical engineering, vol. 14, pp. 1-16, 2012.
• S. Sepeur, Nanotechnology: technical basics and applications. Vincentz Network GmbH & Co KG, 2008.
• J. L. Hernández-Pinero et al., "Effect of heating rate and plant species on the size and uniformity of silver nanoparticles synthesized using aromatic plant extracts," Applied Nanoscience, vol. 6, no. 8, pp. 1183-1190, 2016.
• F. Erci, R. Cakir-Koc, and I. Isildak, "Green synthesis of silver nanoparticles using Thymbra spicata L. var. spicata (zahter) aqueous leaf extract and evaluation of their morphology-dependent antibacterial and cytotoxic activity," Artificial cells, nanomedicine, and biotechnology, pp. 1-9, 2017.
• H. Bar, D. K. Bhui, G. P. Sahoo, P. Sarkar, S. P. De, and A. Misra, "Green synthesis of silver nanoparticles using latex of Jatropha curcas," Colloids and surfaces A: Physicochemical and engineering aspects, vol. 339, no. 1-3, pp. 134-139, 2009.
• J. Parsons, J. Peralta-Videa, and J. Gardea-Torresdey, "Use of plants in biotechnology: synthesis of metal nanoparticles by inactivated plant tissues, plant extracts, and living plants," Developments in environmental science, vol. 5, pp. 463-485, 2007.
• M. Altikatoglu, A. Attar, F. Erci, C. M. Cristache, and I. Isildak, "Geen Synthesis of Copper Oxide Nanoparticles Using Ocimum basilicum Extract and Their Antibacterial Activity," Fresenius environmental bulletin, vol. 26, no. 12 A, pp. 217-222, 2017.
• A. K. Mittal, Y. Chisti, and U. C. Banerjee, "Synthesis of metallic nanoparticles using plant extracts," Biotechnology advances, vol. 31, no. 2, pp. 346-356, 2013.
• V. Armendariz, I. Herrera, M. Jose-yacaman, H. Troiani, P. Santiago, and J. L. Gardea-Torresdey, "Size controlled gold nanoparticle formation by Avena sativa biomass: use of plants in nanobiotechnology," Journal of Nanoparticle Research, vol. 6, no. 4, pp. 377-382, 2004.
• S. Iravani, "Green synthesis of metal nanoparticles using plants," Green Chemistry, vol. 13, no. 10, pp. 2638-2650, 2011.
• G. S. Dhillon, S. K. Brar, S. Kaur, and M. Verma, "Green approach for nanoparticle biosynthesis by fungi: current trends and applications," Critical reviews in biotechnology, vol. 32, no. 1, pp. 49-73, 2012.
• U. Klueh, V. Wagner, S. Kelly, A. Johnson, and J. Bryers, "Efficacy of silver‐coated fabric to prevent bacterial colonization and subsequent device‐based biofilm formation," Journal of Biomedical Materials Research, vol. 53, no. 6, pp. 621-631, 2000.
• Q. L. Feng, J. Wu, G. Chen, F. Cui, T. Kim, and J. Kim, "A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus," Journal of biomedical materials research, vol. 52, no. 4, pp. 662-668, 2000.
• J. S. Kim et al., "Antimicrobial effects of silver nanoparticles," Nanomedicine: Nanotechnology, Biology and Medicine, vol. 3, no. 1, pp. 95-101, 2007.
• J. Hurlow, K. Couch, K. Laforet, L. Bolton, D. Metcalf, and P. Bowler, "Clinical biofilms: a challenging frontier in wound care," Advances in wound care, vol. 4, no. 5, pp. 295-301, 2015.
• M. E. Cortés, J. C. Bonilla, and R. D. Sinisterra, "Biofilm formation, control and novel strategies for eradication," Sci Against Microbial Pathog Commun Curr Res Technol Adv, vol. 2, pp. 896-905, 2011.
• A. J. Huh and Y. J. Kwon, "“Nanoantibiotics”: a new paradigm for treating infectious diseases using nanomaterials in the antibiotics resistant era," Journal of controlled release, vol. 156, no. 2, pp. 128-145, 2011.
• O. V. Singh, Bio-nanoparticles: biosynthesis and sustainable biotechnological implications. John Wiley & Sons, 2015.
• J. R. Morones et al., "The bactericidal effect of silver nanoparticles," Nanotechnology, vol. 16, no. 10, p. 2346, 2005.
• A. Nanda and M. Saravanan, "Biosynthesis of silver nanoparticles from Staphylococcus aureus and its antimicrobial activity against MRSA and MRSE," Nanomedicine: Nanotechnology, Biology and Medicine, vol. 5, no. 4, pp. 452-456, 2009.
• V. babu Nagati, R. Koyyati, M. R. Donda, J. Alwala, K. R. Kundle, and P. R. M. Padigya, "Green synthesis and characterization of silver nanoparticles from Cajanus cajan leaf extract and its antibacterial activity," International Journal of Nanomaterials and Biostructures, vol. 2, no. 3, pp. 39-43, 2012.
• A. M. Awwad, N. M. Salem, and A. O. Abdeen, "Biosynthesis of silver nanoparticles using Olea europaea leaves extract and its antibacterial activity," Nanoscience and Nanotechnology, vol. 2, no. 6, pp. 164-170, 2012.
• K. Shameli et al., "Green biosynthesis of silver nanoparticles using Callicarpa maingayi stem bark extraction," Molecules, vol. 17, no. 7, pp. 8506-8517, 2012.
• J. Liu and R. H. Hurt, "Ion release kinetics and particle persistence in aqueous nano-silver colloids," Environmental science & technology, vol. 44, no. 6, pp. 2169-2175, 2010.
• R. Gengan, K. Anand, A. Phulukdaree, and A. Chuturgoon, "A549 lung cell line activity of biosynthesized silver nanoparticles using Albizia adianthifolia leaf," Colloids and Surfaces B: Biointerfaces, vol. 105, pp. 87-91, 2013
• A. Ahmad et al., "The effects of bacteria-nanoparticles interface on the antibacterial activity of green synthesized silver nanoparticles," Microbial pathogenesis, vol. 102, pp. 133-142, 2017.41, 2015