Yüksek Doz Hidrojen Peroksit ile Muamele Edilen İnsan Nöroblastoma Hücre Hattında Darbeli Elektromanyetik Alan Maruziyetinin Glutatyon Miktarına Etkisi

Year 2022, Volume: 17 Issue: 1, 146 - 154, 27.05.2022

Tuğçe Şimşek Serdar Karakurt , Çiğdem Gökçek-saraç

https://doi.org/10.29233/sdufeffd.1029835

Cited By: 1

Abstract

Darbeli Elektromanyetik Alan (PEMF) elektromanyetik alanların iyonlaştırıcı olmayan formlarından biri olup nörodejeneratif bozuklukların semptomlarının tedavisi gibi çeşitli tıbbi problemler için alternatif bir tedavi olarak kullanılmaktadır. Çalışmanın amacı, yüksek doz hidrojen peroksit (H2O2) ile muamele edilen insan nöroblastoma hücre hattında kısa süreli 75 Hz frekanslı PEMF maruziyetinin glutatyon (GSH) miktarına etkilerini araştırmaktır. Hücreler üç deneysel gruba ayrılmıştır: (I) sham-kontrol; (II) H2O2 ile muamele edilen hücreler; (III) H2O2 muamelesinin ardından PEMF'ye maruz bırakılan hücreler. Hücre canlılığı ve glutatyon miktarı sırasıyla spektrofotometrik ve Yüksek Performanslı Likit Kromatografi (HPLC) teknikleri kullanılarak ölçülmüştür. Yüksek doz H2O2 ile muamele edilen nöroblastoma hücre hattında muamele sonrası PEMF maruziyetinin oksidatif stresin zararlı etkilerine karşı sitoprotektif etkisinin, hücre canlılığında ve GSH miktarında artış ile ilişkili olduğu bulunmuştur.

Keywords

Darbeli elektromanyetik alan, Nöroblastoma, Oksidatif stres, Glutatyon, HPLC

Supporting Institution

AKDENİZ ÜNİVERSİTESİ

Project Number

FBA-2020-5388

Thanks

Bu çalışma Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir (Proje Numarası: FBA-2020-5388).

Effect of Pulsed Electromagnetic Field Exposure on Glutathione Amount in Human Neuroblastoma Cell Line Treated with High-Dose Hydrogen Peroxide

Abstract

The Pulsed Electromagnetic Field (PEMF) is one of the non-ionizing forms of electromagnetic fields that has been used as an alternative therapy for various medical problems, including treating symptoms of neurodegenerative disorders. The present work’s goal is to explore the possible effects of short-term exposure to 75 Hz frequency of PEMF on the amount of glutathione (GSH) in high-dose hydrogen peroxide (H2O2) treated human neuroblastoma cell line. Cells were divided into three experimental groups: (I) sham-control; (II) cells treated by H2O2; (III) cells post-exposed to PEMF following H2O2 challenge. The cell viability and the amount of glutathione were measured by spectrophotometrically and High Performance Liquid Chromatography (HPLC) techniques, respectivelly. It has been found that the cytoprotective effect of post-PEMF exposure against deleterious effects of oxidative stress triggered by high-dose H2O2 challenge in neuroblastoma cell line is mediated by the increase in the cell viability and the elevation in the GSH amount.

Keywords

Pulsed electromagnetic field, Neuroblastoma, Oxidative stress, Glutathione, HPLC

Project Number

FBA-2020-5388

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