Changes in Germination, Antioxidant Enzyme Activities and Biochemical Contents of Safflower (Carthamus tinctorius L.) Under Different Salinity Levels

Year 2022, Volume: 17 Issue: 1, 185 - 194, 27.05.2022

Sercan Önder , Ebru Dayan , Yaşar Karakurt , Muhammet Tonguç

https://doi.org/10.29233/sdufeffd.1090142

Cited By: 2

Abstract

The present study was carried out using Dinçer and Olas safflower varieties at 5 different salt (NaCl) concentrations (0, 50, 100, 150, and 200 mM) for 14 days. The germination percentages of the cultivars under salt conditions as well as the activities of antioxidant enzymes (SOD, CAT, POD and APX) and biochemical changes (protein and MDA) in the seedlings were determined. The germination percentage decreased with increased salt concentrations, and the greatest decrease in germination percentage was observed at a 200 mM salt concentration by 34% in both cultivars. The activity of superoxide dismutase (SOD) increased at low salt concentrations, but decreased after 100 and 150 mM salt concentrations, respectively. Catalase (CAT) and ascorbate peroxidase (APX) activities, as well as malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents, increased with increasing salt concentrations at Dinçer and Olas, but total soluble protein content decreased with increasing salt concentration. Peroxidase (POD) activity was not significantly affected by salt stress in safflower. Germination percentage showed negative correlations with CAT, MDA and H2O2 levels, and showed a positive correlation with soluble protein content under salt stress in safflower. The present results may be useful to identify mechanisms of salt tolerance involving antioxidant enzyme activities and biochemical changes in safflower seedlings.

Keywords

Germination, Lipid peroxidation, Oxidative stress, Protein, Salt stress

Thanks

Sercan Önder was financially supported by the Council of Higher Education under YÖK 100/2000 fellowship program for graduate students. Present research did not receive any grant from funding agencies in the public, commercial, or not-for-profit sectors.

Farklı Tuzluluk Seviyelerinde Aspir’de (Carthamus tinctorius L.) Çimlenme, Antioksidan Enzim Aktiviteleri ve Biyokimyasal Bileşenlerin Değişimi

Abstract

Mevcut çalışma Dinçer ve Olas aspir çeşitleri kullanılarak 5 farklı tuz (NaCl) konsantrasyonunda (0, 50, 100, 150 ve 200 mM) 14 gün boyunca yapılmıştır. Çeşitlerin tuzluluk şartları altında çimlenme oranları ve fideciklerdeki antioksidan enzim aktiviteleri (SOD, CAT, POD ve APX) ve biyokimyasal değişiklikler (protein and MDA) belirlenmiştir. Tuz konsantrasyonu arttıkça çimlenme oranı azalmış ve her iki çeşit için çimlenme oranındaki en yüksek düşüş %34 ile 200 mM tuz konsantrasyonunda gözlenmiştir. Süperoksit dismutaz (SOD) aktivitesi düşük tuz konsantrasyonlarında artmış fakat Dinçer çeşidinde 100 mM ve Olas çeşidinde 150 mM tuz konsantrasyonlarında SOD aktivitesinin azaldığı gözlenmiştir. Dinçer ve Olas çeşitlerinde katalaz (CAT) ve askorbat peroksidaz (APX) aktivitesi, malondialdehit (MDA) ve hidrojen peroksit (H2O2) miktarlarının tuz konsantrasyonlarına bağlı olarak arttığı fakat toplam çözünebilir protein içeriğinin ise azaldığı belirlenmiştir. Peroksidaz (POD) aktivitesi aspirde tuz stresine bağlı olarak önemli bir değişim göstermemiştir. Aspirde tuz stresi sonucu çimlenme yüzdesindeki azalma CAT, MDA ve H2O2 ile negatif, çözünür protein miktarı ile pozitif korelasyon göstermiştir. SOD aktivitesindeki değişim ise POD, CAT, MDA ve H2O2 ile pozitif korelasyonları göstermiştir. Mevcut sonuçlar, aspirin tuzluluk toleransını regüle etmek için fideciklerdeki antioksidan enzim aktiviteleri ve biyokimyasal içeriklerdeki değişimler hakkında yararlı olacaktır.

Keywords

Çimlenme, Lipid peroksidasyon, Oksidatif stres, Protein, Tuz stresi

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