Histochemical Structure of Stomach (Proventriculus and Gizzard) in Some Bird Species

Year 2015, Volume: 19 Issue: 2, 115 - 122, 02.09.2015

Emel Demirbağ , Kenan Çınar , Mehmet Tabur , Reşat Aştı

Abstract

Stomach samples (proventriculus and gizzard) of sparrow hawk (Accipiter nisus), crow (Corvus corone) and sparrow (Passer domesticus) were investigated in this study. In histochemical investigations, strong sulfated mucins were not determined in compound glands of proventriculus, but a little reaction to AB (pH 0.5) in compound glands was observed in proventriculus of sparrow hawk and crow. While weak AB (pH 1.0) (+) character was found in sparrow gizzard, it was found as dense in superficial glands of sparrow hawk. However, it was not observed in crow gizzard. While AF (+) character (sulfated mucins) was detected as dense in epithelium and as weak in glands of gizzard of sparrow hawk, this character was not observed in glands of gizzards of crow and sparrow. Any reactivity to Periodic Acid-Shiff (PAS) staining was not observed in both epithelium and glands of sparrow proventriculus

Keywords

Stomach, Sparrow hawk, Crow, Sparrow, Mucosubstance, Histochemistry

Bazı Kuş Türlerinde Mide (Proventrikulus ve Gizzard)’nin Histokimyasal Yapısı

Abstract

Bu çalışmada atmaca (Accipiter nisus), karga (Corvus corone) ve serçeden (Passer domesticus) alınan mide örnekleri (proventrikulus ve gizzard) incelendi. Histokimyasal incelemelerde, proventrikulusun bileşik bezlerinde güçlü sülfatlı musinlere rastlanmadı; fakat atmaca ve karga proventrikulusunda bileşik bezlerde AB (pH 0.5)’e karşı zayıf bir reaksiyon gözlendi. Serçe gizzardında zayıf AB (pH 1.0) (+) karakter bulunurken, atmacanın superficial bezlerinde yoğundu. Bununla birlikte karga gizzardında bu karaktere rastlanmadı. AF(+) karakterin (sülfatlı
musinler) atmaca gizzardının epitelinde yoğun, bezlerinde zayıf olduğu tespit edilirken; karga ve serçe gizzardlarının bezlerinde bu karakter gözlenmedi. Serçe proventrikulusunun hem epiteli hem de bezlerinde Periyodik Asit-Shiff (PAS) boyamasına karşı hiç bir reaksiyon gözlenmedi.

Keywords

Mide, Atmaca, Karga, Serçe, Mukosubstans, Histokimya

References

• Allen, A. 1981. Structure and function of gastrointestinal mucus. Johnson L. (Editor), Physiology of the gastroenterology Tract, Raven Press, New York. 617-39 pp.
• Altamirano, F., Avila, R., Samar, M.E., de Fabro, S.P. 1984.
• mucosubstances in the chick glandular stomach characterization of J.F.A.
• histochemical uses of periodic acid. Stain Technol, 23: 99-108. Histological
• and Mowry, R.W. 1956. Alcian blue tecniques for the histochemical study of acidic carbohydrates. J. Histochem. Cytochem, 4: 407-408.
• Neutra, M., Forstner, J. 1987. Gastrointestinal mucus: synthesis, secretion, and function. Johnson L (Editor): Physiology of the Gastrointestinal Tract, Chapter 34. Raven Pres, New York.
• Pastor, L.M., Ballesta, J., Madrid, J.F., Perez-Thomas, R., Hernandez, F. 1988. A histochemical study of the mucins in the digestive tract of the chicken. Acta Histochem, 83: 91-97.
• Selvan, P.S., Ushakumary, S., Ramesh, G. 2008. Studies on the histochemistry of the proventriculus and gizzard of post-hatch guinea fowl (Numida meleagris). International Journal of Poultry Science, 7 (11): 1112-1116.
• Shyla, P., Ommer, P.A., Lucy, P. 1992. Structure and post-natal development of the proventriculus in the duck. Ind J Poult Sci, 27: 10-14.
• Spicer, S.S., Mayer, D.R. 1960. Aldehyde fuchsin/alcian blue. Culling CFA, Allison RT and Barr WT (Editors): Cellular Pathology Tecnique, Butterworths, London. 233 pp.
• Straus, G.J., Dekker, J. 1992. Mucin-type glycoproteins. Crit Rev Biochem Mol Biol, 27 (1-2): 57-92.