Determination of Glycoconjugate Content in Brahma (Gallus gallus) Cock’s Testis by Lectin Histochemistry

Year 2016, Volume: 20 Issue: 1, 72 - 79, 15.04.2016

Belgin Kanat , Kenan Çınar

https://doi.org/10.19113/sdufbed.86098

Abstract

This study was aimed to determine some glycoconjugates (α-D-Man, αD-Glc, N-acetyl-D-Galactosamine, Fucα1-2Gal-R, Galactose-β1,3-Nacetylgalactosamine, GlcNAcβ1-4GlcNAcβ1-4GlcNAc, Neu5Ac) in testis tissue of Brahma (Gallus gallus) cock by using some lectins (Con A, SBA, UEA-I, PNA, and WGA) through lectin histochemical method. Lectin histochemistry results indicated that primary spermatocytes reacted very strongly with Con A lectin, but Leydig cells reacted weakly with this lectin. A weak to strong reaction to all glycoconjugates were observed in secondary spermatocytes. A very strong reaction to WGA was noticed in early and late spermatids as WGA lectin did not bind to Leydig cells. A very strong reaction to SBA and PNA was detected in
spermatogonium and the basal lamina of lamina propria. Any UEA-I and SBA lectin labelling was not observed in Leydig cells. Sertoli cells were found to contain plenty of all glycoconjugates intensely

Keywords

Cock, Gallus gallus, Testis, Glycoconjugate, Lectin, Histochemistry

Brahma Horozu (Gallus gallus) Testisindeki Glikokonjugat İçeriğinin Lektin Histokimyası ile Belirlenmesi

Abstract

Bu çalışmada Brahma (Gallus gallus) horoz testisindeki bazı glikokonjugatların (α-D-Man, α-D-Glc, N-asetil-D-Galaktozamin, Fucα1-2Gal-R, Galaktoz-β1,3-N-asetilgalaktozamin, GlcNAcβ1-4GlcNAcβ1-4GlcNAc, Neu5Ac) lektin (Con A, SBA, UEA-I, PNA, WGA) histokimyasal yöntemlerle analizinin yapılması amaçlandı. Uygulanan lektin histokimyasal yöntemler sonucunda primer spermatositlerde Con A lektininde çok güçlü bağlanma gözlenirken Leydig hücrelerinde zayıf bağlanma gözlendi. Sekonder spermatositlerin zayıftan güçlüye kadar tüm glikokonjugatları içerdiği belirlendi. WGA lektinine karşı erken ve geç dönem spermatidlerde çok güçlü reaksiyon gözlenirken Leydig hücrelerinde reaksiyon gözlenmedi. Spermatogonyumlarda ve lamina propriyanın bazal laminasında SBA ve PNA lektinlerine karşı çok güçlü reaksiyon saptandı. UEA-I ve SBA lektin işaretlenmesi Leydig hücrelerinde saptanmadı. Peritubuler hücrelerde zayıf (Con A, UEA-I, SBA) ve orta yoğunlukta (WGA ve PNA) bağlanma saptandı. Sertoli hücrelerinin çok yoğun şekilde tüm glikokonjugatları içerdiği belirlendi.

Keywords

Horoz, Gallus gallus, Testis, Glikokonjugat, Lektin, Histokimya

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