Micropropagation of Gerbera (<i>Gerbera jamesonii Bolus</i>) under Different Color of Light-Emitting Diodes

Year 2016, Volume: 20 Issue: 3, 468 - 474, 22.11.2016

Kürşat Mustafa Gök Bekir Şan , Ahmet Kamil Bayhan

https://doi.org/10.19113/sdufbed.13860

Cited By: 6

Abstract

In present research, the effects of light quality on micropropagation of gerbera were investigated. The MS medium containing 1 mg L^-1 BAP and 0.1 mg L^-1 NAA was used in proliferation stage and the MS medium containing 0.05 mg L^-1 IBA was used in rooting stage of the study. Cultured plants were grown in cabinets illuminated with red, blue, daylight LED lamps separately and their various mixtures. Cool white fluorescent lamp was used as control treatment in the study. The highest ratio of explant with new shoots, the number of shoots per explant and shoot length values were obtained from cool white fluorescent lamps (90%, 5.35 shoots and 2.63 cm, respectivey), 100% blue LED lamp (95%, 5.24 shoots and 2.40 cm, respectively), 100% cool white LED lamp (90%, 4.18 shoots and 2.21 cm, respectively) and 70% red + 30% blue LED lamp treatments (80%, 4.14 shoots and 2.21cm, respectively) in the proliferation stage of the study. These treatments also yielded the highest values in terms of plant fresh and dry weight. While the highest rooting percentage (75%) was obtained from 100% cool white LED light treatment, the differences between the treatments except for 100% red LED light were not significant. Rooted plants were successfully acclimatized to outdoor conditions with 84% survival rate in peat + perlite mixture (2:1).

Keywords

Gerbera, Gerbera jamesonii Bolus; Micropropagation; Light quality; In vitro rooting; Acclimatization

References

• [1] Rogers, M.N., Tjia, B.O. 1990a. Gerbera Production for Cut Flowers and Pot Plants. Timber Press, INC., Portland, Oregon. p 115.
• [2] Rogers, M.N., Tjia, B.O. 1990b. Gerbera Production Timber Press Growers Handbook Series vol 4. ISBN. 0-88192-172-6, Hong Kong.
• [3] Murashige, T., Serpa, M., Jones, J.B. 1974. Clonal Multiplication of Gerbera Through Tissue Culture. Hort Sci, 9, 175-180.
• [4] Shabanpour, K., Sharifi, A., Bagheri, A., Moshtaghi, N. 2011. Effect of Genotypes and Culture Medium on Shoot Regeneration and Proliferation of Gerbera jamesonii. African Journal of Biotechnology, 10(57), 12211-12217.
• [5] Shabbir, K., Ahmad, T., Hafiz, I.A., Hussain, A., Abbasi, N.A., Ahmad, J. 2012. In vitro Regeneration of Gerbera jamesonii cv. Sunglow. African Journal of Biotechnology, 11(42), 9975-9984.
• [6] Cardoso, J.C., Teixeira da Silva, J.A. 2012. Gerbera Micropropagation. Biotechnology Advances, 31, 1344–1357.
• [7] Akter, N., Hoque, M.I., Sarker, R.H. 2012. In vitro Propagation in Three Varieties of Gerbera (Gerbera jamesonii Bolus.) from Flower Bud and Flower Stalk Explants. Plant Tissue Cult and Biotech, 22(2), 143-152.
• [8] Wang, Z., Li, G., He, S., Jaime, A., Silva, T., Tanaka, M. 2011. Effect of Cold Cathode Fluorescent Lamps on Growth of Gerbera jamesonii Plantlets in vitro. Scientia Horticulturae, 130, 482-484.
• [9] Kim, S.J., Hahn, E.J., Heo, J.W., Paek, K.Y. 2004. Effects of LEDs on Net Photosynthetic Rate, Growth and Leaf Stomata of Chrysanthemum Plantlets in vitro. Scientia Horticulturae, 101, 143-151.
• [10] Zhou, Y., Singh, B.R. 2002. Red Light Stimulates Flowering and Anthocyanin Biosynthesis in American Cranberry. Plant Growth Regulation, 38, 165–171.
• [11] Burdett, A.N. 1972. Two Effects of Prolonged Far Red Light on the Response of Lettuce Seeds to Exogenous Gibberellin. Plant Physiol, 49, 531-534.
• [12] Jala, A. 2011. Effects of Different Light Treatments on the Germination of Nepenthes mirabilis. International Transaction Journal of Engineering, Management and Applied Sciences and Technologies, 2(1), 83-91.
• [13] Barrero, J.M., Jacobsen, J.V., Talbot, M.J., White, R.G., Swain, S.M., Garvin, D.F., Gubler, F. 2012. Grain Dormancy and Light Quality Effects on Germination in the Model Grass Brachypodium distachyon. New Phytologist, 193, 376–386.
• [14] Nhut, D.T., Takamura, T., Watanabe, H., Okamoto, K., Tanaka, M. 2003. Responses of Strawberry Plantlets Cultured in vitro Under Superbright Red and Blue Light-Emitting Diodes (LEDs). Plant Cell, Tissue and Organ Culture, 73, 43-52.
• [15] Li, Q., Kubota, C. 2009. Effects of Supplemental Light Quality on Growth and Phytochemicals of Baby Leaf Lettuce. Environmental and Experimental Botany, 67, 59-64.
• [16] Uludağlı, R. 2014. Gisela 6 Kiraz Anacının Mikroçoğaltımı Üzerine Işık Kalitesinin Etkileri. Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü Tarım Makinaları Anabilim Dalı Yüksek Lisans Tezi, 88s, Isparta.
• [17] Murashige, T., Skoog, F. 1962. A Revised Medium for Rapid Growth and Bioassay with Tabacco Tissue Cultures. Physiol Plant, 15, 473-497.
• [18] Lian, M.L., Murthy, H.N., Paek, K.Y. 2002. Effects of Light Emitting Diodes (LEDs) on the in vitro Induction and Growth of Bulblets of Lilium Oriental Hybrid ‘Pesaro’. Scientia Horticulturae, 94, 365-370.
• [19] OuYang, F., Mao, J.F., Wang, J., Zhang, S., Li, Y. 2015. Transcriptome Analysis Reveals that Red and Blue Light Regulate Growth and Phytohormone Metabolism in Norway Spruce [Picea abies (L.) Karst.]. Plos One, 10(8), e0127896. doi:10.1371/ journal.pone.0127896.
• [20] Poudel, P.R., Kataoka, I., Mochioka, R. 2008. Effect of Red and Blue-Light-Emitting Diodes on Growth and Morphogenesis of Rapes. Plant Cell Tissue and Organ Culture, 92, 147-153.
• [21] Gabryszewska, E., Rudnicki, R. 1995. The Influence of Light Quality on the Shoot Proliferation and Rooting of Gerbera jamesonii in vitro. Acta Agrobotanica, 48 (2), 105-111.
• [22] Geng, F., Moran, R., Day, M., Halteman, W., Zhang, D. 2015. In vitro Shoot Proliferation of Apple Rootstocks ‘B.9’, ‘G.30’, and ‘G.41’ Grown under Red and Blue Light. HortScience, 50, 430-433.
• [23] Tanaka, M., Takamura, T., Watanabe, H., Endo, M., Yanagi, T., Okamoto, K. 1998. In vitro Growth of Cymbidium Plantlets Cultured under Super Bright Red and Blue Light-Emitting Diodes (LEDs). Journal of Hort Sci Biotechnol, 73, 39-44.
• [24] Shin, K.S., Murthy, H.N. 2008. The Effect of Light Quality on the Growth and Development of in vitro Cultured Doritaenopsis Plants. Acta Physiol Plant, 30, 339-343.
• [25] Iacona, C., Muleo, R. 2010. Light Quality Affects in vitro Adventitious Rooting and ex vitro Performance of Cherry Rootstock ‘Colt’. Scientia Horticulturae, 125, 630-636.
• [26] Chakrabarty, D., Datta, S.K. 2008. Micropropagation of Gerbera, Lipid Peroxidation and Antioxidant Enzyme Activities During Acclimatization Process. Acta Physiol Plant, 30, 325–331.
• [27] Jun, Z., Mei, Z., Songjun, Z., Kunlin, W., Zhilin, C. 2009. Studies on Regeneration System from Petiole of Gerbera jamesonii Bolus 'Sunanda' in vitro. Journal of Tropical and Subtropical Botany, 17(3), 254-260.