Sorgum Tohumlarında Ağır Metal Stresi Etkilerinin Hafifletilmesinde Bazı Bitki Büyüme Regülatörlerinin Rolü

Year 2017, Volume: 21 Issue: 3, 886 - 893, 13.03.2017

Damla Güvercin

https://doi.org/10.19113/sdufbed.66361

Cited By: 1

Abstract

Bu araştırmada kurşun [Pb(NO₃)₂], çinko [Zn(NO₃)₂.4H₂O] ve kadmiyum [Cd(NO₃)₂.4H₂O] ortamlarındaki Sorghum bicolor L. tohumlarının çimlenmesi üzerindeki ağır metal etkilerinde gibberellik asit (GA₃/G), kinetin (K) ve bu iki bitki büyüme regülatörünün (BBR) kombinasyonlarının (K+G) rolü incelenmiştir. Kurşun (Pb), çinko (Zn) ve kadmiyum (Cd) konsantrasyon artışına paralel olarak tohumların çimlenmesini engellemiştir. Çimlenme üzerinde en zararlı etkiyi Cd yapmış bunu sırası ile Pb ve Zn izlemiştir. BBR uygulamaları Pb ile Cd’nin çimlenmeyi engelleyici etkisini çoğunlukla hafifletemezken (yalnızca 3 mM Pb düzeyindeki G uygulaması ve 0.4, 0.5 ve 0.7 mM Cd düzeylerindeki G uygulaması başarılıdır) Zn’nun yol açtığı bu olumsuzlukları değişik derecede ve önemli ölçüde hafifletmişlerdir. Her üç ağır metal de çimlenme aşamasında radikula uzaması başta olmak üzere koleoptil uzamasını belirgin şekilde inhibe etmiştir. Bu ağır metallere karşı radikula uzaması koleoptil uzamasından daha duyarlı bulunmuştur. BBR uygulamaları bu ağır metallerin radikula uzaması üzerindeki baskısını hafifletmede başarılı olamamışlardır. Pb, Zn ve Cd’nin koleoptil uzaması üzerindeki olumsuz etkilerini hafifletmede K+G başta olmak üzere uygulanan tüm bitki büyüme regülatörleri (BBR) başarı sağlamışlardır. Çalışılan ağır metallerin taze ağırlık ve fidecik su içeriği yüzdesinde yaptıkları olumsuz etkiler K ve K+G başta olmak üzere BBR uygulamaları ile çoğunlukla hafifletilebilmiştir.

Keywords

Sorgum, Ağır metal stresi; Kinetin; Gibberellik asit

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