Grafen Oksitin Ksantin Oksidaz Aktivitesi Üzerine <i>İn Vitro</i> Etkisinin İncelenmesi

Year 2017, Volume: 21 Issue: 2, 401 - 407, 15.08.2017

İsmail Sari , Sevtap Bakır , Veysel Kenan Çelik Serpil Erşan Duygu Anaklı

Abstract

Grafen karbon atomlarının altıgen bir kafes şeklinde düzenlenmesi ile oluşan iki boyutlu bir yapıdır ve biyolojik sistemlerin de dahil olduğu çeşitli uygulama alanlarına sahiptir. Bu çalışmada grafen oksitin (GO) pürin katabolizmasında rol alan ksantin oksidaz (KO) enzim aktivitesi üzerine in vitro etkisinin incelenmesi amaçlanmıştır. GO' nun KO aktivitesi üzerine etkisini incelemek amacıyla 0.50, 1.00, 5.00 ve 10.00 ppm olmak üzere 4 farklı derişimde GO çözeltisi KO tepkime ortamına ilave edilmiş ve 5 farklı substrat (hipoksantin) derişiminde aktivite değerleri spektrofotometrik olarak belirlenmiştir. K_m ve V_max değerleri saptanmıştır. KO' nun V_max değeri 0.83 µM Ürat/mgProtein/dakika, K_m değeri 138.95 µM olarak hesaplanmıştır. Tepkime ortamına 0.50, 1.00, 5.00, 10.00 ppm GO eklendiğinde KO enzimin sırasıyla %36.1, 72.3, 77.1 ve 84.3 oranında inhibe olduğu tespit edilmiştir. Sonuç olarak, GO derişimi arttıkça enzimin hem K_m hem de V_max değerlerinde dikkate değer bir azalma olduğu ve substrat derişimi artırsak da enzim inhibisyonun devam ettiği tespit edilmiştir. Bu da inhibisyon tipinin unkompetetif olduğunu göstermektedir.

Keywords

Grafen oksit, Ksantin oksidaz; Enzim; Enzim aktivitesi

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