<i>Platismatia glauca</i> (L.) W.L.Culb. & C.F.Culb.'nın İnsan Lenfositleri Üzerindeki Biyolojik Aktiviteleri

Year 2018, Volume: 22 Issue: 2, 840 - 848, 15.08.2018

Buğrahan Emsen , Ali Aslan , Abdullah Kaya

https://doi.org/10.19113/sdufbed.00743

Cited By: 1

Abstract

Likenler yapısında farklı metabolitleri bulunduran ve bu bileşenleri sayesinde antik çağlardan beri birçok hastalığın tedavisinde yararlanılan organizma olarak dikkat çekmektedir. Bu yüzden, mevcut çalışma önemli liken türlerinden biri olan Platismatia glauca (L.) W.L.Culb. & C.F.Culb.'nın insan lenfositleri üzerindeki etkilerini incelemeyi amaçlamıştır. P. glauca'dan elde edilen metanol (PME) ve su (PSE) ekstraktlarının hücreler üzerindeki sitotoksik aktiviteleri 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür ve laktat dehidrogenaz testleri ile analiz edilmiştir. PME ve PSE ile muamele edilen lenfositlerdeki oksidatif değişimin belirlenmesi amacıyla toplam antioksidan kapasite (TAK) ve toplam oksidan durum (TOD) analizleri gerçekleştirilmiştir. Bu uygulamalara ek olarak, ekstraktların hücreler üzerinde sebep olduğu genetik hasar hücrelerdeki 8-hidroksi-2′-deoksiguanozin seviyeleri ölçülerek tespit edilmiştir. Hesaplanan IC₅₀ değerleri,PSE'nin (162,26 mg/L) PME'ye (84,02 mg/L) kıyasla çok daha düşük seviyede sitotoksik aktivite gösterdiğini ortaya çıkarmıştır. Her iki ekstraktın tüm konsantrasyonlarının (6,25-200 mg/L) negatif kontrole kıyasla istatistiksel olarak (p>0,05) hücrelerdeki TAK'yi yükselttiği ve aynı zamanda düşük konsantrasyonlarının genetik hasar meydana getirmediği belirlenmiştir. Tüm uygulamalar göz önüne alındığında, PME ve özellikle PSE'nin belli konsantrasyonlarının (<25 mg/L) lenfositler üzerinde anlamlı derecede oksidatif ve genetik hasara neden olmadan TAK'yi yükselttiği ve bu nedenle P. glauca likeninin doğal bir antioksidan olarak kullanılabileceği gözlenmiştir.

Keywords

Antioksidan, Genotoksisite; Oksidatif stres; Sitotoksisite

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