Fusarik Asit ve Enniatin-A Mikotoksinlerinin İnsan Umblikal Ven Endotel Hücreleri (HUVEC) Üzerine Sitotoksik Etkilerinin Değerlendirilmesi

Year 2018, Volume: 22 Issue: Special, 133 - 140, 05.10.2018

Sevcan Mamur

Abstract

Fusarik asit (FA) ve Enniatin-A (EN-A) Fusarium spp. cinsi mikotoksinler olup gıdaları ve yemleri kontamine edebilirler. Tahıllarda mikotoksin varlığı önemli sağlık sorunlarına yol açmakla birlikte ekonomik kayıplara da sebep olmaktadır. Bu çalışmada FA ve EN-A’nın potansiyel sitotoksik etkileri insan umblikal ven endotel (HUVEC) hücre hattında 3-(4,5-dimethylthiazolyl-2)-2,5 diphenyltetrazolium bromid (MTT) testi ile incelenmiştir. Her iki mikotoksinin de farklı konsantrasyonları (FA için 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50, 100, 150, 200, 400 µg/mL, EN-A için 0.048, 0.098, 0.195, 0.39, 0.78, 1.56, 3.125, 6.25 µg/mL) 24 ve 48 saat muamele sürelerinde uygulanmıştır. Sonuç olarak, FA ve EN-A’nın hücre canlılığını (%), her iki muamele süresinde de yüksek konsantrasyonlarda önemli düzeyde düşürdüğü belirlenmiştir. FA’nın 24 saatlik uygulamasında IC₅₀ (hücrelerin %50’sini öldüren) değerinin 150 μg/mL olduğu, 48 saatlik uygulamasında ise 200 μg/mL olduğu tespit edilmiştir. EN-A’nın ise IC₅₀ değerinin 24 saatlik uygulamada 3.125 μg/mL, 48 saatlik uygulamada ise 1.56 μg/mL olduğu saptanmıştır. Bu sonuçlara göre FA ve EN-A’nın yüksek konsantrasyonlarda HUVEC hücrelerinde sitotoksik etki gösterdikleri gözlenmiştir. Ayrıca, EN-A mikotoksininin FA’dan daha fazla toksik etkiye sahip olduğu söylenebilir. Bu sitotoksik etki, hücre döngüsünde ve hücre proliferasyonunda yavaşlamaya  dolayısıyla DNA replikasyonun bozulmasına ve hücrelerde apoptozise neden olabilir.

Keywords

Fusarik asit, Enniatin-A; Mikotoksin; HUVEC hücre hattı; MTT testi

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