Use of Cloud Point Extraction and Spectrophotometric Determination of Carmosine by Applying Experimental Design

Year 2022, Volume: 26 Issue: 3, 398 - 406, 20.12.2022

Tufan Güray , Ülkü Dilek Uysal

https://doi.org/10.19113/sdufenbed.1068593

Abstract

Carmosine (E122) as a food additive is a dye that is often used in food, cosmetics and pharmaceuticals. In this study, Brij 58 was used in the extraction step for the spectrophotometric determination of carmosine (KAR) in syrup sample after cloud point extraction (CPE). Certain parameters such as extraction temperature, pH, Brij 58 (surfactant) concentration and Na2SO4 concentration were optimized using central composite design, Box-Behnken design and classical experimental methods. The determination range of the method was 0.05 – 4.00 g mL-1. The limit of detection (LOD) of this method is 0.0018 μg mL-1 for KAR. The recovery values of KAR were calculated to be between 98.00% and 102.67%. The findings show that this new CPE method can be quickly used for sensitive determination of KAR for routine analysis.

Keywords

Carmosine, Cloud point extraction, Experiment design, Central composite design, Box-Bhenken

Karmosin için Deney Tasarımı Uygulanarak Bulutlanma Noktası Ekstraksiyonu Kullanımı ve Spektrofotometrik Tayini

Abstract

Karmosin (E122) bir gıda katkı maddesi olarak genellikle gıda, kozmetik ve ilaçlarda kullanılan boyadır. Bu çalışmada, bulutlanma noktası ekstraksiyonundan (CPE) sonra şurup numunesinde karmosinin (KAR) spektrofotometrik tayini için ekstraksiyon aşamasında Brij 58 kullanılmıştır. Ekstraksiyon sıcaklığı, pH, Brij 58 (yüzey aktif madde) konsantrasyonu ve Na2SO4 konsantrasyonu gibi bazı parametreler merkezi kompozit tasarımı, Box-Behnken tasarımı ve klasik deneysel yöntemler kullanılarak optimize edilmiştir. Yöntemin doğrusal çalışma aralığı 0,05 - 4,00 g mL-1 olarak belirlenmiştir. Bu yöntemin gözlenebilme sınırı (LOD); KAR için 0,0018 μg mL-1 dir. Yöntemde KAR'ın geri kazanım değerlerinin %98,00 ve %102,67 arasında olduğu hesaplanmıştır. Bulgular, bu yeni CPE yönteminin rutin analizde KAR’ın hassas belirlenmesi için hızlı bir şekilde kullanılabileceğini göstermiştir.

Keywords

Karmosin, Bulutlanma noktası ekstraksiyonu, Deney tasarımı, Merkezi kompozit asarım, Box-Bhenken, Carmosine, Cloud point extraction, Experiment design, Central composite design

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