Serum Metalloproteinase-2 and Tissue İnhibitor Metalloproteinase-1 Levels As a Biomarker in Patients With Osteoarthritis Due To Brucella İnfection

Year 2022, Volume: 13 Issue: 3, 410 - 418, 20.12.2022

Mustafa Güzel , Orhan Akpınar , Veysel Atilla Ayyıldız , Mümtaz Cem Şirin

https://doi.org/10.22312/sdusbed.1138497

Abstract

Objectives: It was aimed to investigate the usability of MMP-2 and TIMP-1 levels as biomarkers in the oosteoarticular complications of brucellosis.
Methods: The subjects were categorized into three groups as the healthy control group, brucella group, and brucella patients with osteoarticular involvement groups. Before medical treatment, serum samples from patients and control groups were stored at -80ºC until the day of study. MMP-2 and TIMP-1 serum levels were quantified by the ELISA method.
Results: Serum level of MMP-2 (mean ± SD) in healthy control group was 1.71 +/- 0.10 ng / mL. Brucella patient group and Osteoarticular complıcation group were 14.3 +/- 2.52 ng / ml 20.65 +/- 2.33 ng / ml respectively (p=0.001). The mean TIMP-1 level in the control group was 3578.96 +/- 67.2 ng / mL, while in the Brucella group, this rate was 998.27 +/- 66.7 ng / mL and in the bone involvement group, 1656. 17 +/- 17.3 ng / ml. The difference between the control group and the brucella patients and the complicated group was statistically significant (p= 0.001).
Conclusions: We think that the significant change in serum levels of MMP-2 and TIMP-1 when evaluated together with the radiological method, can be used as a biochemical indicator of the development of osteoarticular complications.

Keywords

Brucellozis, MMP-2, TIMP-1, biomarker, osteoarticuler complication

Brucella Enfeksiyonuna Bağlı Osteoartritli Hastalarda Biyobelirteç Olarak Serum Metalloproteinaz-2 ve Doku İnhibitörü Metalloproteinaz-1 Seviyeleri

Abstract

Amaç: Brusellozun oosteoartiküler komplikasyonlarında MMP-2 ve TIMP-1 düzeylerinin biyobelirteç olarak kullanılabilirliğinin araştırılması amaçlandı.

Metot: Olgular sağlıklı kontrol grubu, brusella grubu ve osteoartiküler tutulumu olan brusella hastaları olarak üç gruba ayrıldı. Medikal tedavi öncesi hasta ve kontrol gruplarından alınan serum örnekleri çalışma gününe kadar -80ºC'de saklandı. MMP-2 ve TIMP-1 serum seviyeleri, ELISA yöntemiyle ölçüldü.

Bulgular: Sağlıklı kontrol grubunda MMP-2 (ortalama ± SD) serum düzeyi 1,71 +/- 0,10 ng / mL idi. Brusella hasta grubu ve Osteoartiküler komplikasyon grubunda sırasıyla 14,3 +/- 2,52 ng / ml 20,65 +/- 2,33 ng / ml idi (p=0,001). Kontrol grubunda ortalama TIMP-1 düzeyi 3578,96 +/- 67,2 ng/ml iken, Brucella grubunda bu oran 998,27 +/- 66,7 ng/ml, kemik tutulumu grubunda 1656, 17 +/- 17,3 ng / ml idi . Kontrol grubu ile brusella hastaları ve komplike grup arasındaki fark istatistiksel olarak anlamlıydı (p= 0,001).

Sonuç: Radyolojik yöntemle birlikte değerlendirildiğinde, MMP-2 ve TIMP-1 serum seviyelerindeki önemli değişiklik, brusellozun osteoartiküler komplikasyonlarının gelişiminin biyokimyasal bir göstergesi olarak kullanılabileceğini düşünüyoruz.

Keywords

Brusellozis, MMP-2, TIMP-1, Biyobelirteç, osteoartiküler komplikasyon

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