RESVERATROL SIÇANLARDA OKSİDATİF STRESİ VE APOPTOZU BASKILAYARAK METOTREKSAT KAYNAKLI YUMURTALIK HASARINI HAFİFLETİR

Year 2022, , 476 - 484, 30.09.2022

Kübra Kısmet Aka , Çiğdem Aydın Acar , Meltem Özgöçmen , Rahime Aslankoç , Şükriye Yeşilot

https://doi.org/10.17343/sdutfd.1145034

Abstract

Amaç
Dişi sıçanlarda Metotreksat (MTX) kaynaklı yumurtalık
hasarına karşı Resveratrolün (RES) antioksidan ve
antiapoptotik etkilerinin değerlendirilmesi amaçlanmaktadır.
Gereç ve Yöntem
Çalışmada ratlar 7 gruba ayrıldı: Kontrol; MTX (15mg/
kg-1. gün); MTX (1. ve 3. gün); MTX (1., 3. ve 5. gün);
MTX (1. gün) + RES (20mg/kg); MTX (1. ve 3. gün)
+ RES; MTX (1., 3. ve 5. gün) + RES. Grup 4 ve 6'da
sadece bir hayvan kaldığı ve grup 7'de hiç hayvan
olmadığı için bu gruplar değerlendirme dışı bırakıldı.
Yumurtalık hasarı, sıçanların yumurtalık dokularında
hematoksilen-eozin (H-E) ve TUNEL boyaması yapılarak
değerlendirildi. Yumurtalık dokularındaki toplam
oksidan/antioksidan (TOS/TAS) durumu spektrofotometrik
olarak değerlendirildi. Ayrıca kandaki DNA hasarı
comet testi ile değerlendirildi.
Bulgular
Biyokimyasal analizler sonucunda MTX gruplarında,
grup 2 ve 3'te kontrol grubuna göre TOS düzeyleri artarken,
grup 5'te anlamlı olarak azaldı. Öte yandan,
grup 5'te grup 2 ve 3'e göre TAS düzeyi anlamlı olarak
arttı. H-E boyama sonuçları, MTX kaynaklı hasarın
RES (grup 5) tedavisi ile düzeldiğini gösterirken;
maksimum TUNEL pozitif boyama görüntüsü; 2. ve 3.
gruplarda eşit miktarlarda, ardından 5. grupta gözlemlenmiştir.
Ayrıca DNA hasarını gösteren comet skoru
2. ve 3. gruplarda anlamlı olarak artarken, 5. grupta
önemli ölçüde azalmıştır.
Sonuç
Bu çalışmanın sonuçları doğrultusunda, Resveratrol’ün
antioksidan özelliğinden dolayı Metotreksat’a
bağlı yumurtalık hasarına karşı koruyucu etki sağlayabileceği
görülmüştür.

Keywords

Metotreksat, Resveratrol, yumurtalık hasarı, antioksidan, rat

Supporting Institution

Burdur Mehmet Akif Ersoy Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

0615-YL-19

RESVERATROL ALLEVIATES METHOTREXATE-INDUCED OVARIAN INJURY VIA SUPPRESSING OXIDATIVE STRESS AND APOPTOSIS IN RATS

Abstract

Objective
The study aimed to evaluate the antioxidant and
antiapoptotic effects of Resveratrol (RES) against
Methotrexate (MTX)-induced ovarian damage in
female rats and were divided into 7 groups:
Material and Method
Control; MTX (15mg/kg-1st day); MTX (1st and 3rd
day); MTX (1st, 3rd and 5th day); MTX (1st day) +
RES (20mg/kg); MTX (1st and 3rd day) + RES; MTX
(1st, 3rd and 5th day) + RES. Since there was only
one animal left in groups 4 and 6 and no animals
in group 7, these groups were excluded from the
evaluation. Ovarian damage was evaluated by
performing hematoxylin-eosin (H-E) and TUNEL
staining on ovarian tissues of rats. Total oxidant/
antioxidant status in ovarian tissues was evaluated
spectrophotometrically. In addition, DNA damage in
blood was assessed by comet assay.
Results
As a result of biochemical analyzes, TOS levels
increased in MTX groups, group 2 and 3, compared
to the control group, while TOS level decreased
significantly in group 5. On the other hand, TAS
level increased significantly in group 5 compared
to groups 2 and 3. H-E staining results showed that
MTX-induced damage improved with RES (group 5)
treatment. Maximum TUNEL positive staining image;
it was observed in equal amounts in groups 2 and
3, followed by group 5. In addition, the comet score,
which indicates DNA damage, increased significantly
in groups 2 and 3, while it decreased significantly in
group 5.
Conclusion
In line with the results of this study, it was observed
that Resveratrol may provide protective effects
against Methotrexate-induced ovarian damage due to
its antioxidant properties.

Keywords

Methotrexate, Resveratrol, ovarian damage, rat, antioxidant

Project Number

0615-YL-19

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