Elektrik alanın DNA Hasarı ve Beyin Dokusu Üzerine Etkileri - Astaksantin’in Rolü

Year 2020, Volume: 27 Issue: 2, 166 - 172, 01.06.2020

Rahime Aslankoç , Oğuzhan Kavrık Özlem Özmen

https://doi.org/10.17343/sdutfd.583950

Abstract

Özet

Bu çalışmada, uzun süreli elektrik alan
(EA) maruziyetinin lenfosit DNA hasarı ve beyin dokusu üzerine etkilerini
araştırmayı amaçladık. Doğal bir karetonoid pigmenti olan astaksantin’in (AST)
EA’nın zararlı etkilerini azaltabileceğini düşündük. Çalışmada, 30 adet Wistar Albino (3-4 aylık) dişi
sıçanlar kullanıldı. Sıçanlar her grupta 10 hayvan olmak üzere rastgele üç
gruba ayrıldı; Grup I (Kontrol), Grup II (EA uygulanan grup - 10 kV/m EA, 30
gün, günde 23 saat), Grup III (EA+AST tedavi grubu - 10 kV/m EA, 30 gün, günde
23 saat + 100 mg/kg/gün AST, 30 gün,
gavaj). Sıçanlar deney sonunda sakrifiye edildi. Kan ve beyin dokusu
biyokimyasal analizler için toplandı. Kan dokuda comet assay yöntemi ile
lenfosit DNA hasarı analizi, beyin dokuda malondialdehit (MDA) seviyesi,
süperoksit dismutaz (SOD) ve katalaz (CAT) enzim aktiviteleri çalışıldı. EA
uygulanan grupta kontrol grubuna göre MDA ve comet skoru yüksek bulundu. AST
uygulanan grupta EA grubuna göre MDA ve comet skoru azalırken SOD ve CAT enzim
aktiviteleri arttı. Çalışma sonuçları EA’ın kan dokuda lenfosit DNA hasarına ve
beyin dokuda oksidatif strese neden olduğunu gösterdi. Ayrıca AST tedavisinin
lenfosit DNA hasarını ve oksidatif stresi azalttığını gösterdi. 

Keywords

Elektrik alan, Oksidaitif stres

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