THE IMMUNOHISTOCHEMICAL EXPRESSIONS OF MISMATCH REPAIR GENES MLH1, PMS2, MSH6, MSH2 IN GASTRIC CANCER; A TISSUE MICROARRAY STUDY

Year 2021, Volume: 28 Issue: 3, 487 - 497, 13.09.2021

Gamze Erkılınç , Nermin Karahan , Şirin Başpınar , Zümrüt Arda Kaymak , Şehnaz Evrimler

https://doi.org/10.17343/sdutfd.866797

Abstract

Objective
We aimed to evaluate the correlation between the
immunohistochemical expressions of MLH1, PMS2,
MSH6, MSH2 and clinicopathological parameters in
gastric carcinoma.
Matherials and Methods
Immunohistochemistry was performed on the tissue
microarray (TMA) sections of 103 primary gastric
adenocarcinoma and 27 gastric mucosal tissue
samples without tumor. All markers were evaluated
for the presence of nuclear staining. Negative expression
in any of the markers was accepted as a
deficiency. Then, the comparison was made between
the two subgroups as; deficient mismatch repair
(dMMR) and proficient mismatch repair (pMMR).
Results
The histopathological subtypes as intestinal and
non-intestinal, the intestinal group showed significant
deficient expression of MSH2 compared with
the non-intestinal group. PMS2 expression was significantly
higher in the other subtypes than signet ring
cell carcinoma. Also, we observed that the loss of
MLH1 and PMS2 expressions were higher in moderately/
poor differantiated tumors than the well differantiated
ones. Perineural invasion was significantly
higher in patients with loss of MLH1, MSH6, PMS2
expression and dMMR compared to patients with
pMMR. There was no significant difference between
dMMR and pMMR when compared the groups
who received chemotherapy/ radiotherapy and who
did not. There was not found significant relationship
between MLH1, MSH2, MSH6, PMS2 expressions
and survival.
Conclusion
We found a significant relationship between perineural
invasion and the loss of expression of MLH1,
MSH6 and PMS2. PMS2 expression was also significantly
higher in the other subtypes of GC than signet
ring cell carcinomas.

Keywords

Gastric adenocarcinoma, MLH1, PMS2, MSH2, MSH6

Supporting Institution

none

Project Number

-

Thanks

none

HATALI EŞLEŞME GENLERİNDEN MLH1, PMS2, MSH6, MSH2’İN MİDE KANSERLERİNDE İMMÜNHİSTOKİMYASAL EKSPRESYONU; BİR DOKU MİKROARRAY ÇALIŞMASI

Abstract

Amaç
Mide kanserinde MLH1, PMS2, MSH6, MSH2’in immünhistokimyasal
ekspresyonları ile klinikopatolojik
parametrelerin arasındaki ilişkiyi değerlendirmeyi
amaçladık.
Gereç ve Yöntem
Yüzüç primer mide adenokarsinom ve tümörsüz 27
mide mukozasına ait doku mikroarray (DMA) kesitlerine
immünhistokimyasal uygulama yapıldı. Tüm
markerlar nükleer boyanma açısından değerlendirildi.
Markerlardan herhangi birinde negatiflik eksiklik
olarak kabul edildi. Daha sonra hatalı eşleşme genlerinde
eksiklik var (dMMR) ve hatalı eşleşme genleri
sağlam (pMMR) olmak üzere 2 alt grup arasında
karşılaştırma yapıldı.
Bulgular
Histopatolojik olarak intestinal ve intestinal olmayan
alt tiplerinden, MSH2’nin intestinal grupta intestinal
olmayan gruba göre ekspresyonunda anlamlı kayıp
gözlendi. PMS2 ekspresyonu taşlı yüzük hücreli karsinomda
diğer alt tiplere göre anlamlı olarak yüksekti.
Ayrıca MLH1 ve PMS2 ekspresyonlarının kaybının
orta/kötü diferansiye tümörlerde, iyi diferansiye
tümörlere göre daha yüksek olduğunu gözlemledik.
MLH1, MSH6, PMS2 ekspresyon kaybı ve dMMR
olan olgularda pMMR’li olgulara göre perinöral invazyon
anlamlı şekilde daha yüksekti. Kemoterapi/
radyoterapi alan ve almayan gruplar karşılaştırıldığında
dMMR ve pMMR arasında anlamlı fark yoktu.
MLH1, MSH2, MSH6, PMS2 ekspresyonları ile sağkalım
arasında anlamlı ilişki bulunmadı.
Sonuç
Perinöral invazyon ile MLH1, MSH6 ve PMS2 ekspresyon
kaybı arasında anlamlı ilişki bulduk. PMS2
ekspresyonu taşlı yüzük hücreli karsinomda diğer alt
tiplere göre anlamlı olarak yüksekti.

Keywords

Mide adenokarsinom, MLH1, MSH2, PMS2, MSH6

Project Number

-

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