EXCESSIVE USE OF FLAXSEED MAY POSE A THREAT TO KIDNEY TISSUE: AN EXPERIMENTAL STUDY

Year 2023, Volume: 30 Issue: 1, 89 - 96, 14.03.2023

İlkay Armağan , Şükriye Yeşilot

https://doi.org/10.17343/sdutfd.1247065

Abstract

Objective
We aimed to investigate the effects of flaxseed,
which is widely used due to its healing effects, on the
kidney tissue when the daily recommended dose is
exceeded.
Material and Methods
We divided the male Wistar Albino rats into four
groups of eight, namely the control, OD-1 (1.4 mg/
kg/d), OD-2 (2.8 mg/kg/d), and OD-3 (5.6 mg/kg/d)
groups. Ground flaxseed was administered to each
overdose group by oral gavage for 7 days. At the
end of the experiment, blood and kidney samples
were collected. Blood samples were centrifuged and
serum was separated for renal function analyses.
The half of the kidney samples were stored at -20
°C until biochemical analyses and we fixed the
remaining tissues with 10% buffered formalin for
histopathological analysis.
Results
Serum urea and BUN levels were significantly
increased in all overdose groups compared to the
control group (for all p˂0.05). TAS levels significantly
decreased in the OD-3 group compared to the control
(p=0.044). There was an increase renal TOS levels
in all overdose groups compared to the control
and also, OSI level of the OD-3 group significantly
increased compared to the control (p=0.016). While
histopathological changes were not observed in OD-1
and OD-2 groups compared to the control group,
there were non-significant histopathological changes
in OD-3 group.
Conclusion
It was concluded that the use of flaxseed above the
recommended daily dose can pose a threat to kidney
tissue. So, while being used for its benefit, in order not
to be exposed to its toxic effects, overuse and longterm
use should be avoided.

Keywords

Flaxseed, Kidney, Overdose

KETEN TOHUMUNUN AŞIRI KULLANIMI BÖBREK DOKUSU İÇİN TEHDİT OLUŞTURABİLİR: DENEYSEL BİR ÇALIŞMA

Abstract

Amaç
İyileştirici etkisi nedeniyle yaygın olarak kullanılan
keten tohumunun önerilen günlük doz aşıldığında,
böbrek dokusu üzerindeki etkilerini araştırmayı amaçladık.
Gereç ve Yöntem
Erkek Wistar Albino cinsi ratlar her grupta 8 adet olacak
şekilde 4 gruba ayrıldı. Gruplar: kontrol, OD-1 (1,4
mg/kg/gün), OD-2 (2,8 mg/kg/gün) ve OD-3 (5,6 mg/
kg/gün) şeklinde isimlendirildi. Her bir doz aşımı olan
gruba, 7 gün boyunca oral gavaj yolu ile öğütülmüş
keten tohumu verildi. Deney sonunda kan ve böbrek
örnekleri alındı. Kan örnekleri santrifüjlenerek böbrek
fonksiyon analizleri için serumlarına ayrıldı. Böbrek
örneklerinin yarısı -20 °C'de biyokimyasal analizler
yapılana kadar saklandı ve kalan dokular histopatolojik
analiz için %10 tamponlu formalin ile fikse edildi.
Bulgular
Serum üre ve BUN seviyeleri, kontrol grubuyla karşılaştırıldığında,
doz aşımı olan tüm gruplarda önemli
ölçüde arttı (tümü için p˂0.05). TAS seviyeleri,
OD-3 grubunda kontrole göre anlamlı olarak azaldı
(p=0,044). Tüm aşırı doz gruplarında renal TOS düzeylerinde
kontrole göre artış vardı ve ayrıca OSI düzeyi,
OD-3 grubunda kontrole göre anlamlı düzeyde
arttı (p=0,016). OD-1 ve OD-2 gruplarında kontrol grubuna
göre histopatolojik değişiklikler gözlenmezken,
OD-3 grubunda anlamlı olmayan histopatolojik değişiklikler
belirlendi.
Sonuç
Önerilen günlük dozun üzerinde keten tohumu kullanımının
böbrek dokusu için tehdit oluşturabileceği
kanısına varıldı. Bu nedenle yararı için kullanılırken,
toksik etkilerine maruz kalmamak için aşırı ve uzun
süreli kullanımdan uzak durulmalıdır.

Keywords

Keten tohumu, Böbrek, Doz aşımı

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