INVESTIGATION OF THE EFFECT OF OLANZAPINE AND L-CARNITINE ON RAT TESTIS TISSUE

Year 2023, Volume: 30 Issue: 3, 284 - 293, 23.09.2023

Merve Akkuş , Faruk Kılıç , Halil Aşcı , Fatma Nihan Cankara , Mehtap Savran , Dilek Ulusoy Karatopuk

https://doi.org/10.17343/sdutfd.1121445

Abstract

Objective
Olanzapine has adverse effects on sexual dysfunction.
There are a limited number of studies showing that it
also causes structural changes in the reproductive
system. This study aimed to examine the effects of
L-Carnitine on testicular damage caused by Olanzapine
in terms of histopathological, sperm parameters, and
biochemical aspects.
Material and Method
A total of 48 adult Sprague-Dawley male rats were
used in our study. Rats were divided into 6 groups,
8 rats in each group: the Control group (C), 200 mg/
kg L-Carnitine administered group (LC), 2 mg/kg low
dose Olanzapine administered group (LOZN), 2 mg/kg
Olanzapine, and 200 mg/kg L-Carnitine administered
group (LOZN+LC), 4 mg/kg Olanzapine administered
group (HOZN), 4 mg/kg Olanzapine and 200 mg/
kg L-Carnitine administered group (HOZN+LC).
Testicular tissues were used for the determination of
MDA, IL-1B, IL-6, TAS, TOS levels, and histological
evaluation.
Results
In the groups receiving Olanzapine, a decrease in
sperm count, an increase in MDA, IL-1β, TOS values,
and dose-dependent histopathological changes in
testicular tissue were observed. Histopathological
changes were found at a lower rate in the Olanzapine
and L-Carnitine administered groups compared to the
Olanzapine-only groups.
Conclusion
It was concluded that the degenerative histological
findings in the testicular structure observed in the
high-dose Olanzapine administered groups might be
caused by the oxidative stress induced by Olanzapine
in the testicular tissue. L-Carnitine, on the other hand,
was thought to be effective in reducing testicular
degeneration by reducing oxidative damage.

Keywords

Olanzapine, L-Carnitine, testis, sperm, oxidative stress

Supporting Institution

This study was supported by the Süleyman Demirel University Scientific Research Project Office

Project Number

TTU-2018-6750.

Thanks

We thank Süleyman Demirel University Scientific Research Project Office for their support in this study.

OLANZAPİN VE L-KARNİTİNİN RAT TESTİS DOKUSU ÜZERİNDEKİ ETKİSİNİN ARAŞTIRILMASI

Abstract

Amaç
Olanzapinin cinsel işlev bozukluğu üzerine olumsuz
etkileri vardır. Üreme sisteminde de yapısal değişikliklere
neden olduğunu gösteren sınırlı sayıda çalışma
bulunmaktadır. Bu çalışmada Olanzapinin neden olduğu
testis hasarı üzerine L-Karnitin'in etkilerinin histopatolojik,
sperm parametreleri, biyokimyasal açıdan
incelenmesi amaçlanmıştır.
Gereç ve Yöntem
Çalışmamızda toplam 48 adet erişkin Sprague-Dawley
erkek sıçan kullanıldı. Sıçanlar, her grupta 8 sıçan
olacak şekilde 6 gruba ayrıldı: Kontrol grubu (C), 200
mg/kg L-Karnitin uygulanan grup (LC), 2 mg/kg düşük
doz Olanzapin verilen grup (LOZN), 2 mg/kg Olanzapin
ve 200 mg/kg L-Karnitin verilen grup (LOZN+LC),
4 mg/kg Olanzapin verilen grup (HOZN), 4 mg/kg
Olanzapin ve 200 mg/kg L-Karnitin verilen grup (HOZN+
LC). MDA, IL-1B, IL-6, TAS, TOS düzeylerinin belirlenmesi,
histolojik değerlendirme için testis dokuları
kullanıldı.
Bulgular
Olanzapin alan gruplarda sperm sayısında azalma,
MDA, IL-1β, TOS değerlerinde artış, testis dokusunda
doza bağlı histopatolojik değişiklikler gözlendi.
Olanzapin ve L-Karnitin uygulanan gruplarda sadece
Olanzapin verilen gruplara göre histopatolojik değişiklikler
daha düşük oranda bulundu.
Sonuç
Yüksek doz Olanzapin uygulanan gruplarda gözlenen
testis yapısındaki dejeneratif histolojik bulguların,
Olanzapinin testis dokusunda oluşturduğu oksidatif
stresten kaynaklanabileceği sonucuna varıldı. L-Karnitin
ise oksidatif hasarı azaltarak testis dejenerasyonun
azaltılmasında etkili olabileceği düşünüldü.

Keywords

Olanzapin, L-Karnitin, testis, sperm, oksidatif stres

Project Number

TTU-2018-6750.

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