OVER SERÖZ KARSİNOMUNDA VE BORDERLİNE SERÖZ TÜMÖRDE İMMÜNHİSTOKİMYASAL PINCH/LIMS-1 ANTİKOR EKSPRESYONUNUN KLİNİKOPATOLOJİK DEĞERİ

Year 2023, Volume: 30 Issue: 3, 592 - 601, 23.09.2023

Onur Ertunç , Özlem Erdem , Zümrüt Arda Kaymak

https://doi.org/10.17343/sdutfd.1349330

Abstract

Amaç
Özellikle ilginç cys-his zengin proteinin (PINCH/LIMS-
1), tümörlerdeki ve tümörle ilişkili stromadaki kanser
hücrelerinin gelişimini ve yayılımını denetlediği varsayılmaktadır.
Bu çalışmanın amacı, seröz borderline
tümör (SBT) ve seröz karsinomda (SC) tümör ve peritümöral
stromadaki PINCH-1 ekspresyonunu değerlendirmek
ve ekspresyonu ile çeşitli klinik ve patolojik
parametreler arasındaki ilişkileri incelemektir.
Gereç ve Yöntem
Yirmi bir SBT ve 89 SK vakasında PINCH-1 antikorunun
ekspresyonu streptavidin/HRP-biotin ile indirekt
immünoperoksidaz tekniği kullanılarak analiz edilmiştir.
PINCH-1'in tümör ve peritümöral stromadaki
boyanma paterni semikantitatif skorlama yöntemi
kullanılarak değerlendirilmiştir. Çalışmada kullanılan
boyama yöntemi PINCH-1 ekspresyonunun tanımlanmasına
olanak sağlamış ve semikantitatif skorlama
yöntemiyle PINCH-1 boyanmasının yaygınlığı ve
yoğunluğu değerlendirilmiştir. Böylece, PINCH-1 ekspresyonu
ile hasta yaşı, tümör boyutu, FIGO evresi,
intraabdominal yıkama sitolojisi, kapsül invazyonu,
tümör yerleşimi, tümör derecesi ve tanı anındaki kanser
antijen 125 (CA125) seviyeleri gibi çeşitli klinik ve
patolojik faktörler arasındaki korelasyon incelenmiştir.
Bulgular
Çalışmada PINCH-1'in SK vakalarında SBT vakalarına
göre daha yaygın olduğu bulunmuştur. SK’ler SBT
vakalarındakilere göre daha güçlü boyanma göstermiştir
(p<0,001). Çalışmada ayrıca, tümörü çevreleyen
dokudaki PINCH-1 boyanmasının yaygınlığı ve
yoğunluğu ile tümörün tek ya da bilateral overde yer
alması arasında pozitif bir korelasyon bulunmuştur
(dağılım için p = 0,038, yoğunluk için p = 0,024). Bununla
birlikte, PINCH-1 boyanması ile tümör boyutu
arasında negatif bir korelasyon vardı (yaygınlık için
p=0.019, yoğunluk için p=0.007). Ayrıca, PINCH-1 tümör
boyanmasının yoğunluğu, FIGO evresi ve tümör
dereceleri arttıkça istatistiksel anlamlılık sergilemiştir
(sırasıyla p = 0,032 ve p = 0,001).
Sonuç
Bu çalışmanın sonuçları, SK'lerin SBT'lere göre daha
yüksek düzeyde PINCH-1 boyanma yoğunluğu sergilediğini
göstermektedir. Ayrıca, FIGO evresindeki
ve tümör derecesindeki artış, tümör dokusunda artan
PINCH-1 boyanma yoğunluğu ile ilişkilidir. Peritümöral
stromadaki PINCH-1 boyanmasının yayılımı ve yoğunluğu
bilateral tümör vakalarında daha dikkat çekicidir,
ancak tümör boyutuyla ters orantılıdır. PINCH-1
ekspresyonu ile önemli klinikopatolojik faktörler arasında
gözlenen ilişki, bu molekülün seröz over kanseri
gelişiminde rol oynayabileceğini düşündürmektedir.
Çalışmamız PINCH-1'in tümörogenezdeki rolünün
daha iyi anlaşılmasına katkıda bulunabilir ve bu yolağı
hedefleyen yeni terapötik stratejilerin geliştirilmesi için
yol gösterici olabilir.

Keywords

FIGO evresi, PINCH/LIMS-1, Seröz borderline tümör, Seröz karsinom, Seröz over karsinomunda PINCH ekspresyonu

CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR

Abstract

Objective
Particularly interesting cys-his rich protein (PINCH/
LIMS-1), a protein implicated in cell adhesion, is
assumed to oversee the development and invasion of
cancer cells in tumors and tumor-associated stroma.
This study aimed to assess PINCH-1 expression in
serous borderline tumor (SBT) and serous carcinoma
(SC) in the tumor and peritumoral stroma and
scrutinize any associations between its expression
and various clinical and pathological parameters.
Material and Method
In this study, the expression of the PINCH-1 antibody
was analyzed in 21 cases of SBT and 89 cases of
SC using the indirect immunoperoxidase technique
with streptavidin/HRP-biotin. The staining pattern of
PINCH-1 in the tumor and peritumoral stroma was
evaluated using a semiquantitative scoring method.
The staining procedure used in the study allowed for
the accurate identification of PINCH-1 expression,
and the data obtained through the semiquantitative
scoring method provided a reliable of assessing
the degree and intensity of PINCH-1 staining. Thus,
the correlation between PINCH-1 expression and
various pathologic factors such as patient age, tumor
size, FIGO stage, intra-abdominal washing cytology,
capsule invasion, tumor location in the ovary, tumor
grade, and cancer antigen 125 (CA125) levels at the
time of diagnosis was examined.
Results
The study found that PINCH-1 was more prevalent
in cases of SC than in SBT cases. The tumors in SC
cases had stronger staining than those in SBT cases
(p<0.001). The study also found a positive correlation
between the diffusiveness and intensity of PINCH-1
staining in the tissue surrounding the tumor and
whether the tumor was located on one or both sides of
the ovaries (p = 0.038 for diffusiveness, p = 0.024 for
intensity). However, there was a negative correlation
between PINCH-1 staining and tumor size (p=0.019 for
diffusiveness, p=0.007 for intensity). Furthermore, the
intensity of PINCH-1 tumor staining exhibited statistical
significance in Figo stage and tumor grades as these
increased (p = 0.032 and p = 0.001, respectively).
Conclusion
The results of this study indicate that SCs exhibit a
higher level of PINCH-1 staining intensity than SBTs.
Furthermore, an increase in the FIGO stage and
tumor grade is associated with increased intensity
of PINCH-1 staining in the tumor tissue. Additionally,
the diffusiveness and intensity of PINCH-1 staining
in the peritumoral stroma is more remarkable in
cases of bilateral tumors but is inversely correlated
with tumor size. The observed association between
PINCH-1 expression and important clinicopathologic
factors suggests that this molecule may be involved
in developing serous ovarian cancer. Overall, these
findings may contribute to a better understanding of
the role of PINCH-1 in ovarian tumorigenesis and may
have implications for developing novel therapeutic
strategies targeting this pathway.

Keywords

FIGO stage, PINCH/LIMS-1, PINCH expression in serous ovarian carcinoma, Serous borderline tumor, Serous carcinoma

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