3 FARKLI DENTİN HASSASİYETİ GİDERİCİ DİŞ MACUNUNUN SİTOTOKSİSİTESİNİN DEĞERLENDİRİLMESİ

Year 2021, Volume: 8 Issue: 2, 460 - 466, 31.08.2021

Ayşe Canan Tutku Çelik , Türkay Kölüş

https://doi.org/10.15311/selcukdentj.696055

Abstract

Amaç: Hassasiyet giderici diş macunları uygulama süreleri boyunca dişler ve oral dokularla sürekli temas halindedirler. Macunların içindeki ajanlar ideal olarak oral dokulara zarar vermemeli, dokuların iyileşmelerine yardımcı olmalıdırlar. Bu çalışmanın amacı, dentin hassasiyeti gidermek için sıklıkla kullanılan üç farklı diş macunun L929 fare fibroblast hücrelerinin canlılıkları üzerine etkilerini değerlendirmektir.
Gereç ve Yöntemler: Test edilen macunların orijinal ekstraktı Dulbecco's Modified Eagle Medium(DMEM) kültür ortamı ile seyreltilerek üç farklı konsantrasyonu hazılandı. L929 fare fibroblastı hücreleri macunların % 25, % 12, % 6’lık üç farklı konsantrasyonuna maruz bırakıldı (n=12). Hücre canlılığı MTT testi ile değerlendirildi. Kontrol grubunun hücre canlılığı %100’e eşitlendi, veriler istatistiksel olarak one-way ANOVA ve post-hoc Tukey’s HSD testleriyle değerlendirildi.
Bulgular: Test edilen macunların % 25, % 12, % 6’lık üç farklı konsantrasyonun da kontrol grubuyla karşılaştırıldığında L929 hücreleri üzerine sitotoksik etkileri olduğu gözlenmiştir (p<0.05). Materyallerin hücre canlılık değerleri arasında yüzdesel olarak anlamlı bir fark olduğu görülmüştür (p<0.05).
Sonuç: Bu çalışmanın sonuçlarına göre test edilen hassasiyet giderici diş macunlarının tümü farklı derecede sitotoksik etkiler göstermiştir. In vitro olarak tamamen tüm kompleks ağız ortamını taklit etmek mümkün olmadığı için elde edilen veriler sınırlıdır. Bu çalışmanın daha detaylı hücre kültür testleri ve klinik testlerle desteklenmesi gerekmektedir.

Keywords

Biyoyuyumluluk, hassasiyet giderici diş macunları, sitotoksisite

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