The Relationship Between Plasma MicroRNAs and Serum Mercury Levels in Patients with Amalgam Filling and Dentists

Year 2021, Volume: 8 Issue: 3, 736 - 743, 31.12.2021

Makbule Tuğba Tuncdemır , Fatma Hümryra Yerlikaya

https://doi.org/10.15311/selcukdentj.800489

Cited By: 1

Abstract

Background: The aim of this study was to investigate the relationship between serum mercury and some plasma microRNA (miRNA) levels associated with neurological diseases in patients with amalgam filling and dentists.
Methods: This study included 30 patients with amalgam filling, 30 dentists, and 30 healthy individuals as the control group. Circulating plasma miRNAs (124-3p, 125-5p, and 127-3p) were evaluated using real-time quantitative polymerase chain reaction analysis. The serum mercury levels were measured using inductively coupled plasma-mass spectrometry. ANOVA and Tukey’s multiple comparison tests were used for statistical analyses (α =.05).
Results: A significant difference in serum mercury and plasma miRNA levels was found between the groups. Significant positive correlations between serum mercury and plasma miRNA 125-5p and 127-3p levels were detected in the patient group (r: 0.56 and r: 0.39, respectively). Serum mercury and plasma miRNA-125-5p levels showed a positive correlation in the dentist group (r: 0.37).
Conclusion: Having amalgam filling caused a significant increase in serum mercury and plasma miRNA levels. The presence of a positive correlation suggests that sensitivity to neurological diseases may increase because of mercury exposure. Alternative restorative materials should be preferred for amalgam filling and amalgam fillings should be replaced with other restorative materials under necessary preventions.

Keywords

dental amalgam, mercury, restorative material

Supporting Institution

Necmettin Erbakan University, Scientefic Research Section

Project Number

181224003

Amalgam Dolgulu Hastalarda ve Diş Hekimlerinde Plazma MikroRNA'lar ile Serum Civa Düzeyleri Arasındaki İlişki

Abstract

Amaç: Bu çalışmanın amacı, amalgam dolgusu olan hastalarda ve diş hekimlerinde serum civa ve nörolojik hastalıklarla ilişkili bazı plazma mikroRNA (miRNA) seviyeleri arasındaki ilişkiyi araştırmaktır.
Gereç ve Yöntemler: Bu çalışmaya 30 amalgam dolgulu hasta, 30 diş hekimi ve 30 sağlıklı kontrol grubu dahil edildi. Dolaşımdaki plazma miRNA'lar (124-3p, 125-5p ve 127-3p), gerçek zamanlı kantitatif polimeraz zincir reaksiyonu analizi kullanılarak değerlendirildi. Serum civa seviyeleri, endüktif olarak eşleşmiş plazma-kütle spektrometresi kullanılarak ölçüldü. İstatistiksel analizler için ANOVA ve Tukey’in çoklu karşılaştırma testleri kullanıldı (α = .05).
Bulgular: Gruplar arasında serum civa ve plazma miRNA seviyelerinde anlamlı bir fark bulundu. Hasta grubunda serum civa ile plazma miRNA 125-5p ve 127-3p seviyeleri arasında anlamlı pozitif korelasyonlar tespit edildi (sırasıyla r: 0.56 ve r: 0.39). Serum civa ve plazma miRNA-125-5p seviyeleri, diş hekimi grubunda pozitif bir korelasyon gösterdi (r: 0.37).
Sonuç: Amalgam dolguya sahip olmak serum civa ve plazma miRNA düzeylerinde önemli bir artışa neden olmuştur. Pozitif bir korelasyonun varlığı, civa maruziyeti nedeniyle nörolojik hastalıklara duyarlılığın artabileceğini göstermektedir. Amalgam dolgu için alternatif restoratif materyaller tercih edilmeli ve amalgam dolgular, gerekli önlemler alınarak diğer restoratif materyaller ile değiştirilmelidir.

Keywords

Dental amalgam, civa, restoratif materyal

Project Number

181224003

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