Transferability of barley and wheat EST-microsatellite markers in some Poaceae members

Year 2016, Volume: 1 Issue: 2, 102 - 113, 31.12.2016

HÜLYA Sipahi , Yeliz Aslan Ayşen Yumurtacı

Abstract

The cross species transferability of  barley and wheat microsatellite markers
developed from expressed sequence tag (EST) libraries constructed under Fusarium infection conditions were detected among 17 species including 8
from Aegilops, 6 from Triticum, Zea mays, Avena sativa, Oryza
sativa.

Transferability rates of barley
microsatellite primer pairs ranged from 29% to 100%. A maximum of 100%
cross-genera transferability noticed with Avena followed by Zea (92%),
Triticum (83%), Aegilops (68%), and Oryza (8%). Primer
pairs were highly transferable within species of Triticum (100% in T.
turgidum durum durum, 92% in T. turgidum durum dicoccon and T.
monococcum aegilopoides, 83% in T.timopheevii timopheevii and T.
turgidum dicoccoides, 67% in T. timopheevii armeniacum). Only one
primer pairs (contig624) showed
100 % cross-species/genera amplification in all materials studied.

Considering wheat microsatellites, the microsatellite
primer pairs were highly transferable within species of Triticum (ranged
from %100 to %70) and but low transferable in the allied cereals (15% in
Avena, 50% in Oryza, 45% in Zea, 60% in Hordeum). Two
primer pairs have shown transferability only in some Triticum species,
while two others showed amplication only in species of Aegilops and Triticum.
Only one primer pairs showed 100 % cross-species/genera amplification in all
materials studied.

This higher
cross-species transferability of EST microsatellite markers indicated a high
level of conservation of DNA sequences belonging to the transcribed region of
the genome and its suitability in comparative genome mapping, genetic diversity
and phylogenetics analysis.

Keywords

Microsatellite markers, transferability

Bazı Poaceae üyelerinde arpa ve buğday EST-mikrosatellit markırlarının aktarılabilirliği

Abstract

Fusariım enfeksiyon koşulları altında oluşturulmuş eksprese olan
dizi (EST) kütüphanelerinden geliştirilen arpa ve buğday mikrosatellit
markırlarının türler/cinsler arası aktarılabilirlikleri, 8 Aegilops, 6 Triticum türü ile,
Zea mays, Avena sativa ve
Oryza sativa ‘yı içeren 17 türde belirlenmiştir. Arpa mikrosatellit primerleri
için aktarılabilirlik oranı %29 ila %100 arasında değişmiştir. Cinsler arası en
yüksek (%100) aktarılabilirlik  Avena
da not edilmiş, bunu Zea (%92) , Triticum (%83),
Aegilops (68%) ve Oryza (%8) takip etmiştir. Primer çiftleri,  Triticum türleri içerinde oldukça
yüksek aktarılabilirdir (T. turgidum durum durum da %100, T. turgidum
durum dicoccon ve T. monococcum aegilopoides da %92,T.timopheevii
timopheevii ve T. turgidum dicoccoides de %83, T. timopheevii armeniacum
da %67). Yalnızca bir primer
çifti (contig624) çalışılan tüm materyallerde, %100 tür/cinsler arası
çoğaltım göstermiştir.

Buğday mikrosatellitleri
değerlendirildiğinde, mikrosatellit primer çiftleri Triticum türleri içinde
oldukça aktarılabilirdir (%70 ila %100 arasında) ancak akraba tahıllarda düşük
aktarılabilirdir (Avena da 15%, Oryza da 50%, Zea da 45%, Hordeum
da 60%). İki primer çifti yalnızca bazı Triticum türlerinde
aktarılabilirlik göstermişken, diğer iki primer çifti sadece Aegilops ve
Triticum türlerinde çoğaltım göstermiştir.

EST mikrosatellite
markırlarının bu yüksek tür/cinsler arası aktarılabilirliği, genomun ifade
edilen bölgesine ait DNA diziliminin yüksek derecede korunduğunu ve bunların
karşılaştırılmalı genom haritalamalarında, genetik çeşitlilikte ve filogenetik
analizlerde uygunluklarını göstermektedir.

Keywords

mikrosatellit marker, aktarılabilirlik, tahıl

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