Deneysel Renal İskemi Reperfüzyon Modelinde Akciğer Dokusunda Oksidan Hasarın İncelenmesi ve Klorojenik Asit (CGA) Kullanımının Koruyucu Etkileri

Year 2018, Volume: 8 Issue: 2, 260 - 265, 29.06.2018

Derya Güzel , Ayhan Tanyeli

https://doi.org/10.31832/smj.423683

Abstract

Amaç: Bu
çalışmanın amacı, sıçan akciğer dokusunda iskemi-reperfüzyona bağlı doku hasarı
üzerine Klorojenik Asit (CGA) etkisini değerlendirmektir.

Gereç ve Yöntemler: Otuz iki adet Wistar Albino
cinsi dişi sıçan temin edildi. Bu sıçanlar randomize olarak dört gruba eşit
olarak bölündü (n=8). Oluşturulan gruplar; sham (S), iskemi-reperfüzyon (IR), 5
mg/kg Klorojenik Asit (CGA5) ve 10 mg/kg Klorojenik Asit (CGA10) uygulanan grup
olarak tanımlanarak cerrahi işlemlere tabi tutuldu. Sham grubundaki sıçanlar
sırt bölgesinden açılarak sağ nefrektomi yapıldı. Sham hariç diğer gruplardaki
sıçanların sırt bölgesi açılıp sağ nefrektomi yapıldıktan sonra sol renal arter
klemplendi. Klorojenik asit iskemiden 10 dk önce ve reperfüzyondan 10 dk önce
olmak üzere iki doz şeklinde intraperitoneal olarak uygulandı. Sol böbreğe 1
saatlik iskemiden sonra 24 saatlik reperfüzyon uygulandı. İşlemler
tamamlandıktan sonra akciğer dokularında total antioksidan düzeyi (TAS), total
oksidan düzeyi (TOS), süperoksit dismutaz (SOD), malondialdehit (MDA) ve
myeloperoksidaz (MPO) seviyeleri spektrofotometrik yöntemlerle ölçüldü.
Oksidatif stres indeksi (OSI) hesaplandı.

Bulgular: IR grubunda S ile karşılaştırıldığında TAS ve SOD değeri
düşerken, TOS, MDA ve MPO değerleri yükseldi. CGA uygulanan gruplarda TAS ve
SOD değerinde yükselme, TOS, MDA ve MPO değerlerinde ise düşme gözlendi.

Sonuç: CGA tedavisinin renal iskemi reperfüzyona bağlı akciğerde
gelişen oksidatif stres hasarının azaltılmasında etkili olabileceği
düşünülmektedir.

Keywords

Klorojenik asit, renal iskemi reperfüzyon, akciğer.

References

• 1. Yun, Y., et al., Ischemic postconditioning modified renal oxidative stress and lipid peroxidation caused by ischemic reperfusion injury in rats. Transplant Proc, 2009. 41(9): p. 3597-602.2. Fadillioglu, E., et al., Melatonin treatment against remote organ injury induced by renal ischemia reperfusion injury in diabetes mellitus. Arch Pharm Res, 2008. 31(6): p. 705-12.3. Anner, H., et al., Pulmonary leukosequestration induced by hind limb ischemia. Annals of surgery, 1987. 206(2): p. 162.4. Stallone, R.J., R.C. Lim, and F.W. Blaisdell, Pathogenesis of the pulmonary changes following ischemia of the lower extremities. The Annals of thoracic surgery, 1969. 7(6): p. 539-549.5. Windsor, A., et al., Role of the neutrophil in adult respiratory distress syndrome. BJS, 1993. 80(1): p. 10-17.6. Rangan, U. and G.B. Bulkley, Prospects for treatment of free radical-mediated tissue injury. Br Med Bull, 1993. 49(3): p. 700-18.7. Liu, Y.H., et al., Gastroprotective effect of andrographolide sodium bisulfite against indomethacin-induced gastric ulceration in rats. Int Immunopharmacol, 2015. 26(2): p. 384-91.8. Demirpence, O., et al., Serum paraoxonase, TAS, TOS and ceruloplasmin in brucellosis. Int J Clin Exp Med, 2014. 7(6): p. 1592-7.9. Aycicek, A. and A. Ipek, Maternal active or passive smoking causes oxidative stress in cord blood. Eur J Pediatr, 2008. 167(1): p. 81-5.10. Santana-Galvez, J., L. Cisneros-Zevallos, and D.A. Jacobo-Velazquez, Chlorogenic Acid: Recent Advances on Its Dual Role as a Food Additive and a Nutraceutical against Metabolic Syndrome. Molecules, 2017. 22(3).11. Ohkawa, H., N. Ohishi, and K. Yagi, Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry, 1979. 95(2): p. 351-358.12. Erel, O., A new automated colorimetric method for measuring total oxidant status. Clinical biochemistry, 2005. 38(12): p. 1103-1111.13. Sun, Y., L.W. Oberley, and Y. Li, A simple method for clinical assay of superoxide dismutase. Clinical chemistry, 1988. 34(3): p. 497-500.14. Dasta, J.F., et al., Costs and outcomes of acute kidney injury (AKI) following cardiac surgery. Nephrol Dial Transplant, 2008. 23(6): p. 1970-4.15. Perico, N., et al., Delayed graft function in kidney transplantation. Lancet, 2004. 364(9447): p. 1814-27.16. Roodnat, J.I., et al., Ischemia times and donor serum creatinine in relation to renal graft failure. Transplantation, 2003. 75(6): p. 799-804.17. Ozlulerden, Y., et al., The renoprotective effects of mannitol and udenafil in renal ischemia-reperfusion injury model. Investig Clin Urol, 2017. 58(4): p. 289-295.18. Katugampola, S. and A. Davenport, Emerging roles for orphan G-protein-coupled receptors in the cardiovascular system. Trends Pharmacol Sci, 2003. 24(1): p. 30-5.19. Gutteridge, J.M., Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem, 1995. 41(12 Pt 2): p. 1819-28.20. Girotti, A.W., Lipid hydroperoxide generation, turnover, and effector action in biological systems. J Lipid Res, 1998. 39(8): p. 1529-42.21. Lavelli, V., C. Peri, and A. Rizzolo, Antioxidant activity of tomato products as studied by model reactions using xanthine oxidase, myeloperoxidase, and copper-induced lipid peroxidation. J Agric Food Chem, 2000. 48(5): p. 1442-8.22. Suzuki, A., et al., Chlorogenic acid attenuates hypertension and improves endothelial function in spontaneously hypertensive rats. J Hypertens, 2006. 24(6): p. 1065-73.23. Bonita, J.S., et al., Coffee and cardiovascular disease: in vitro, cellular, animal, and human studies. Pharmacol Res, 2007. 55(3): p. 187-98.24. dos Santos, M.D., et al., Evaluation of the anti-inflammatory, analgesic and antipyretic activities of the natural polyphenol chlorogenic acid. Biol Pharm Bull, 2006. 29(11): p. 2236-40.25. Feng, R., et al., Inhibition of activator protein-1, NF-kappaB, and MAPKs and induction of phase 2 detoxifying enzyme activity by chlorogenic acid. J Biol Chem, 2005. 280(30): p. 27888-95.26. Bao, L., et al., Chlorogenic acid prevents diabetic nephropathy by inhibiting oxidative stress and inflammation through modulation of the Nrf2/HO-1 and NF-kB pathways. Int Immunopharmacol, 2018. 54: p. 245-253.27. Kim, S.H., et al., Chlorogenic acid suppresses lipopolysaccharideinduced nitric oxide and interleukin1beta expression by inhibiting JAK2/STAT3 activation in RAW264.7 cells. Mol Med Rep, 2017. 16(6): p. 9224-9232.28. Rebai, O., et al., Differential Molecular Targets for Neuroprotective Effect of Chlorogenic Acid and its Related Compounds Against Glutamate Induced Excitotoxicity and Oxidative Stress in Rat Cortical Neurons. Neurochem Res, 2017. 42(12): p. 3559-3572.29. Xi, Y., et al., Effects of chlorogenic acid on capacity of free radicals scavenging and proteomic changes in postharvest fruit of nectarine. PLoS One, 2017. 12(8): p. e0182494.30. Liu, C.C., et al., Chlorogenic acid prevents inflammatory responses in IL1betastimulated human SW1353 chondrocytes, a model for osteoarthritis. Mol Med Rep, 2017. 16(2): p. 1369-1375.31. Ali, N., et al., Protective effect of Chlorogenic acid against methotrexate induced oxidative stress, inflammation and apoptosis in rat liver: An experimental approach. Chem Biol Interact, 2017. 272: p. 80-91.32. Budryn, G., et al., Influence of the Form of Administration of Chlorogenic Acids on Oxidative Stress Induced by High fat Diet in Rats. Plant Foods Hum Nutr, 2017. 72(2): p. 184-191.33. Han, D., et al., Cytoprotective effect of chlorogenic acid against hydrogen peroxide-induced oxidative stress in MC3T3-E1 cells through PI3K/Akt-mediated Nrf2/HO-1 signaling pathway. Oncotarget, 2017. 8(9): p. 14680-14692.34. Ye, H.Y., et al., Chlorogenic Acid Attenuates Lipopolysaccharide-Induced Acute Kidney Injury by Inhibiting TLR4/NF-kappaB Signal Pathway. Inflammation, 2017. 40(2): p. 523-529.35. Akila, P. and L. Vennila, Chlorogenic acid a dietary polyphenol attenuates isoproterenol induced myocardial oxidative stress in rat myocardium: An in vivo study. Biomed Pharmacother, 2016. 84: p. 208-214.