P-COUMARIC ACID REDUCES RENAL ISCHEMIA REPERFUSION-INDUCED ACUTE LUNG INJURY

Year 2018, Volume: 8 Issue: 3, 644 - 649, 30.09.2018

Ayhan Tanyeli , Derya Güzel

https://doi.org/10.31832/smj.456353

Abstract

Objective: This study
investigated the effects of p-coumaric acid (p-CA) on acute lung injury in rats
induced by renal ischemia reperfusion injury.

Materials and Methods: Thirty-two Wistar Albino female rats, 200-250 g for 12-16 weeks, were
randomized to sham (S), renal ischemia-reperfusion (IR), IR with 50 mg / kg
p-coumaric acid p-CA 50) and 100 mg / kg p-coumaric acid (p-CA 100) with IR.
After the rats were opened in the other rats except sham and right nephrectomy
was performed, the left renal artery was clamped and ischemia followed by
reperfusion protocol and p-coumaric acid treatment. Total antioxidant (TAS),
total oxidant (TOS), superoxide dismutase (SOD), malondialdehyde (MDA) and
myeloperoxidase (MPO) levels were measured spectrophotometrically in lung
tissue after the experimental protocol was completed. Oxidative stress index
(OSI) was calculated.

Findings: In the IR group,
TOS, MDA, MPO and OSI significantly increased, while TAS and SOD levels
decreased (p<0.001). Increase in TAS and SOD levels and decrease in TOS, MDA
and MPO values ​​were observed in p-CA applied groups (p<0.001).

Conclusion: According to the
results obtained, is shown by biochemical methods in which p-CA treatment
reduces renal ischemia-reperfusion-induced acute lung injury.

Keywords

p-coumaric acid, acute lung injury, ischemic injury

P-Kumarik Asit, Renal İskemi Reperfüzyon Kaynaklı Akut Pulmoner Hasarı Azaltır

Abstract

Amaç: Bu
çalışma ile sıçanlarda böbrek iskemi reperfüzyon hasarı  ile meydana gelen akut akciğer hasarı ve bu
oksidan hasarda p-kumarik asit’in (p-CA) etkisi araştırıldı.

Gereç ve Yöntemler: 12-16 haftalık 200-250 gr
otuz iki adet Wistar Albino cinsi dişi sıçanlar sham (S), renal
iskemi-reperfüzyon (R-IR), R-IR ile 50 mg/kg p-CA (p-CA 50) ve R-IR ile 100
mg/kg p-CA (p-CA 100) uygulanan gruplar olarak sınıflandırıldı. Sham hariç
diğer gruplardaki sıçanların sırt bölgesi açılıp sağ nefrektomi yapıldıktan
sonra sol renal arter klemplenerek iskemi ve takiben reperfüzyon protokolü ile p-CA
tedavisi uygulandı. Deney protokolü tamamlandıktan sonra akciğer dokusunda
total antioksidan status (TAS), total oksidan status (TOS), süperoksit dismutaz
(SOD), malondialdehit (MDA) ve myeloperoksidaz (MPO) seviyeleri
spektrofotometrik yöntemlerle ölçüldü. Oksidatif stres indeksi (OSI)
hesaplandı.

Bulgular: R-IR grubunda, TOS, MDA, MPO ve OSI anlamlı
düzeyde yükselirken, TAS ve SOD seviyeleri azaldı (p<0.001). p-CA uygulanan
gruplarda TAS ve SOD düzeylerinde artış, TOS, MDA ve MPO değerlerinde ise azalma
gözlendi (p<0.001).

Sonuç: Elde edilen sonuçlarla,
böbrek iskemi reperfüzyon aracılı akut akciğer hasarını p-CA tedavisinin
azalttığı biyokimyasal yöntemlerle gösterilmiştir. 

Keywords

p-CA, akut akciğer hasarı, iskemik hasar

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