Dişi Sıçanlarda Üç Farklı Stres Modelinin Anksiyete ve/veya Depresyon Benzeri Davranışlara Etkilerinin Karşılaştırılması

Year 2019, Volume: 9 Issue: 1, 131 - 140, 20.03.2019

Zafer Şahin , Aynur Koç , Raviye Özen Koca Hatice Solak Alpaslan Özkürkçüler Pınar Çakan Zülfikare İşık Solak Görmüş Selim Kutlu

Abstract

Amaç: Mevcut çalışmada yetişkin dişi sıçanlarda üç farklı
stres protokolünün anksiyete/depresyon benzeri davranışlara etkisinin
kıyaslanması amaçlanmıştır.

Gereç ve Yöntemler: Yetişkin Wistar sıçanlar kontrol, immobilizasyon
stresi-1 (günlük 45 dakika) immobilizasyon stresi-2 (günlük iki kez 45 dakika)
ve sosyal izolasyon (metabolik kafeste) olarak rastgele dört gruba ayrıldı (n =
8). Stres protokolleri 10 gün boyunca uygulandı ve depresyon/anksiyete
davranışları, hayvanlar diöstrüs dönemindeyken, açık alan ve zorunlu yüzme
testi ile değerlendirildi. Aynı testleri 10 günlük dinlenme süresinden sonra
tekrarlandı.

Bulgular: Açık alan testinde, merkez alanda geçirilen zaman
immobilizasyon stresi-2 ve sosyal izolasyon gruplarında azaldı (p<0.05).
Toplam kat edilen mesafe immobilizasyon stresi-1 (p<0.01) ve sosyal
izolasyon (p<0.05) gruplarında daha düşüktü. Sosyal izolasyon grubunun şahlanma davranışı (p < 0.05) kontrol grubuna göre
düşüktü. İmmobilizasyon stresi-1 ve sosyal izolasyon gruplarında yüzme
davranışı azalırken (p < 0.01),
immobilizasyon davranışı kontrol grubuna göre artış göstermişti (p <0.05).
İkinci testlerde, merkezi alanda geçirilen süre immobilizasyon stresi-1 ve
immobilizasyon stresi-2 gruplarında daha düşüktü (p <0.05 ve p <0.01).
İmmobilizasyon stresi-1 (p <0.001) ve sosyal izolasyon gruplarında (p <0.01) yüzme davranışı azalırken,
immobilizasyon süresi kontrole kıyasla artmıştı (p <0.001).

Sonuç: İmmobilizasyon stresi-1 ve metabolik kafeste sosyal
izolasyon protokolü uygulanan erişkin dişi sıçanlarda depresyon benzeri
davranışların daha baskın olduğu söylenebilir. 

Keywords

immobilizasyon stresi, sosyal izolasyon, depresyon, anksiyete, dişi sıçan

Comparison of Effects of Three Distinct Stress Models on Anxiety and/or Depression-Related Behaviors in Female Rats

Abstract

Objective: The aim of the present study was to compare the
effects of three distinct stress protocols on anxiety/depression-related
behaviors in adult female rats.

Material
and Methods: Adult Wistar
rats were randomly divided into four groups (n=8) as control, immobilization
stress-1 (daily 45 minutes) immobilization stress-2 (daily twice 45 minutes)
and social isolation (rats were housed in a metabolic cage). Stress protocols
were performed for a period of 10 days. When the animals were in diestrus,
depression/anxiety-like behaviors were evaluated by the open field test and
forced swimming test. The same tests were repeated after a 10-day rest period.

Results: In the open field test, a percentage of time spent
in the central area was lower in the immobilization stress-2 and social
isolation (p<0.05) groups and total distance was lower in the immobilization
stress-1 (p<0.01) and the social isolation groups (p<0.05). Rearing score
was lower in the social isolation group (p<0.05). Swimming behaviors were lower
(p<0.01), and immobility durations were higher (p<0.05) in the
immobilization stress-1 and social isolation groups. In the second tests, time
spent in the central area was lower in the immobilization stress-1 (p<0.05)
and the immobilization stress-2 (p<0.01) groups. Swimming behaviors were
lower and immobility behaviors were higher in the immobilization stress-1 group
(p<0.001) and the social isolation group (p<0.01 and p<0.001,
respectively).

Conclusion: We suggest that depression-like behaviors are more
dominant in the immobilization stress-1 and social isolation groups of adult
female rats.

Keywords

immobilization stress, social isolation, depression, anxiety, female rat

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