BORIC ACID AMELIORATES LIVER INJURY IN RAT INDUCED BY CYCLOPHOSPHAMIDE

Year 2023, Volume: 13 Issue: 2, 210 - 216, 30.06.2023

Gözde Özge Önder , Özge Göktepe , Eda Okur , Özge Cengiz Mat , Demet Bolat , Esra Balcıoğlu , Arzu Yay

https://doi.org/10.31832/smj.1246705

Abstract

Introduction: The aim of the current study was to look into any potential ameliorative benefits of boric acid on liver damage in rats caused by cyclophosphamide (CTX).
Materials and Methods: Four groups; control, boric acid, CTX, and boric acid + CTX, were created. Female Wistar albino rats were given daily injections of CTX (75 mg/kg) to create the liver damage model. Cyclophosphamide (75 mg/kg) was administered intraperitoneally, and boron (1.3 g/rat/day) was administered by gavage every day for two weeks in the boric acid+CTX group. The histopathological changes were evaluated in ovarian tissue staining with hematoxylin and eosin, masson tricrom, and periodic acid Schiff. We assessed ovarian tissue enzyme activity as malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px).
Results: Images in the boric acid+CTX group had lower histological evaluations than those in the CTX group under the light microscope. According to the findings, boric acid reduced the levels of malondialdehyde (MDA) in the liver tissues. Additionally, boric acid improved the actions of oxidative stress indicators to reduce oxidative stress brought on by CTX and upregulated antioxidant parameters.
Conclusion: In conclusion, our study have demonstrated that CTX- induced liver injury can be alleviated by reducing the tissue MDA levels, increasing the liver’s SOD, GSH-Px, and CAT activities. In order to reduce the liver damage caused by CTX, boric acid may be administered as a dietary supplement or functional food.

Keywords

Boric acid, Cyclophosphamide, Liver injury

BORİK ASİT SIÇANLARDA SİKLOFOSFAMİDİN NEDEN OLDUĞU KARACİĞER HASARINI İYİLEŞTİRİR

Abstract

Amaç: Mevcut çalışmanın amacı, borik asidin sıçanlarda siklofosfamidin (CTX) neden olduğu karaciğer hasarı üzerindeki olası iyileştirici etkilerini araştırmaktır.
Yöntem ve Gereçler: Çalışmada dört grup oluşturuldu; kontrol, borik asit, CTX ve borik asit+CTX. Karaciğer hasarı modelini oluşturmak için Wistar albino cinsi dişi ratlara günlük 75 mg/kg CTX intraperitoneal (i.p.) olarak verildi. Borik asit+CTX grubuna 75 mg/kg CTX (i.p.) ve 1,3 g/rat/gün boron gavaj ile iki hafta boyunca her gün verildi. Over dokusundaki histopatolojik değişiklikler hematoksilen ve eozin, masson trikrom ve periyodik asit-Schiff boyamaları ile değerlendirildi. Over dokusunda malondialdehit (MDA), süperoksit dismutaz (SOD), katalaz (CAT) ve glutatyon peroksidaz (GSH-Px) enzim aktiviteleri belirlendi.
Bulgular: Işık mikroskobunda borik asit+CTX grubundaki histolojik değişikliklerin CTX grubuna göre daha düşük olduğu belirlendi. Bulgulara göre borik asit, karaciğer dokularındaki MDA seviyesini azalttığı gözlendi. Buna ek olarak, borik asit, CTX'in neden olduğu oksidatif stresi azaltmak için oksidatif stres belirteçlerinin etkilerini iyileştirdiği ve antioksidan parametrelerini artırdığı belirlendi.
Sonuç: Sonuç olarak, çalışmamız, CTX'in neden olduğu karaciğer hasarının, doku MDA seviyelerinin düşürülmesi, karaciğerin SOD, GSH-Px ve CAT aktivitelerinin arttırılması ile hafifletilebileceğini göstermiştir. CTX'in neden olduğu karaciğer hasarını azaltmak için borik asit, diyet takviyesi veya fonksiyonel gıda olarak verilebilir.

Keywords

Borik asit, Karaciğer hasarı, Siklofosfamid

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