Determination of Genetic Diversity among Phaseolus vulgaris (Leguminosae) Populations Collected from Konya Region by SDS-PAGE

Year 2010, Volume: 2 Issue: 35, 59 - 64, 01.12.2010

Elif Gülbahçe , Buğra Özkubat Emine Arslan

Abstract

This study aimed to determine the genetic diversity among 17 Phaseolus vulgaris populations collected from districts of Konya by SDS-PAGE. Seed storage protein profiles were scored visually as present (1) or absent (0). In all, 37 polypeptide bands of different sizes ranging from 14.4 to ~135 kDa were observed in the 17 Phaseolus vulgaris populations. Cluster analysis was performed using the UPGMA in Bio 1 D ++ computer program. Similarity among genotypes was estimated basing on Nei’s homology and a dendrogram was obtained. According to dendrogram, 17 populations belonging to Phaseolus vulgaris were similar in rates ranging from 68 to 100 %. While the highest similarity was found between Kanada (Çumra) and Sarnıç (Altınekin) genotypes, the most distant genotype was Sarhoş (Washington) (Derbent).

Keywords

Phaseolus vulgaris, Genetic diversity, SDS-PAGE, Seed storage protein

Konya Bölgesinden Toplanan Phaseolus vulgaris (Leguminosae) Populasyonları Arasındaki Genetik Çeşitliliğin SDS-PAGE Yöntemi ile Belirlenmesi

Abstract

Bu çalışmada Konya’nın ilçelerinden toplanan 17 Phaseolus vulgaris populasyonu arasındaki genetik çeşitliliğin SDS-PAGE ile belirlenmesi amaçlanmıştır. Tohum depo protein profilleri görsel şekilde var (1) ya da yok (0) olarak skorlanmıştır. On yedi Phaseolus vulgaris populasyonunda, 14.4 ve ~135 kDa arasında değişen farklı büyüklüklerde toplamda 37 polipeptit bant gözlenmiştir. Bio1D++ bilgisayar programında UPGMA kullanılarak kümeleme analizi yapılmıştır. Genotipler arasındaki benzerlik Nei’nin homolojisine dayanarak tahmin edilmiş ve bir dendrogram elde edilmiştir. Dendrograma göre Phaseolus vulgaris’e ait 17 populasyon %68-100 arasında değişen oranlarda benzer bulunmuştur. En yüksek benzerlik Kanada (Çumra) ve Sarnıç (Altınekin) genotipleri arasında bulunurken, en uzak genotip Sarhoş (Washington) (Derbent) olmuştur.

Keywords

Phaseolus vulgaris, Genetik çeşitlilik, SDS-PAGE, Tohum depo protein

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