Lipase production from thermophilic bacteria using waste frying oil as substrate

Year 2019, Volume: 9 Issue: 3, 23 - 27, 01.11.2019

Mustafa Özkan Baltacı , Elanur Tuysuz , Hakan Ozkan Mesut Taskın , Ahmet Adıguzel

https://doi.org/10.35354/tbed.510140

Cited By: 2

Abstract

Lipases are widely used in textile, food, medical and chemical
industries. The present study was performed to produce lipase from thermophilic
bacterial strains using waste frying oil as substrate. Among four bacterial
strains, Bacillus licheniformis A7
(GenBank accesion number: KC310458) was determined to be the best lipase producer.
A frying oil concentration of 40 mL/L, temperature of 55 °C, initial pH of 6.0
and incubation time of 72 h were found to be optimal for lipase production from
Bacillus sp. Under the optimal culture conditions, the maximum cell growth and
lipase activity were determined as 2.219 0D_600nm and 1607 U/L,
respectively. 

Keywords

Bacillus licheniformis, Lipase, Waste frying oil

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