Effects of the entomopathogenic fungus, Beauveria bassiana, with adipokinetic hormone, on Myzus persicae and Trialeurodes vaporariorum

Year 2023, Volume: 14 Issue: 2, 105 - 120, 15.01.2024

İsmail Karaca , Ozlem (kalkar) Güven , Umesh Kumar Gautam , Tuğçe Özek

https://doi.org/10.31019/tbmd.1314013

Abstract

The aim of this study was to investigate the effects of the co-application of entomopathogenic fungi (EMFs) and adipokinetic hormone (AKH) on the green peach aphid, Myzus persicae, and the greenhouse whitefly, Trialeurodes vaporariorum. Single and combined applications of two Beauveria bassiana isolates with AKH were tested. The green peach aphid was more susceptible to treatments than the greenhouse whitefly. Mortality rates of 58%, 67%, %95, and 95% were observed for the single and combined applications of BMAUM M6-4 and BMAUM LD 2016, respectively, against M. persicae. The application of these fungi singly and in combination with AKH against T. vaporariorum caused mortality rates of 44%, 68% and 45% and78%, respectively. The protein level measured at 595 nm with the standard, Bovine Serum Albumin (BSA), for M. persicae and T. vaporariorum revealed that the highest level of nutrient mobilization was for the BMAUM M6-4+AKH treatment. The level of carbohydrate (glucose) was slightly higher in the AKH treatments when compared to the control group. The lowest glucose level was measured for the entomopathogenic fungi application, and highest level was determined for the isolates combined with AKH. The mechanism of action of AKH applied with B. bassiana isolates is not fully understood. Therefore, more detailed studies are needed in this area to determine the potential of this promising approach for inclusion in IPM programs for the control of these insect pests.

Keywords

Adipokinetic Hormone, Beauveria bassiana, Entomopathogen fungi, Myzus persicae, Trialeurodes vaporariorum

Supporting Institution

The Scientific and Technological Research Council of Turkey (TUBITAK) 1002 and TUBITAK 2216 (U.K.G.). Tuğçe ÖZEK: This Author is a 100/2000 YÖK PhD Scholar.

Project Number

No. 218O240

Thanks

The authors thanks to employees of the Plant Protection Department of Isparta University of Applied Sciences, Turkey for their material and facility support. Special thanks to Dalibor KODRÍK (Biology Centre, CAS, and Faculty of Science, Institute of Entomology, University of South Bohemia, Branišovská, České Budějovice, Czech Republic) providing some materials and insect hormone.

Entomopatojen fungus, Beauveria bassiana’nın Myzus persicae ve Trialeurodes vaporariorum üzerindeki etkisinin adipokinetik hormon ile arttırılması

Abstract

Bu çalışmanın amacı, adipokinetik hormon (AKH) ile birlikte uygulanan entomopatojen fungusların etkilerini araştırmaktır. Adipokinetik hormon ile tek ve kombine uygulanan Beauveria bassiana izolatları yeşil şeftali yaprak biti, Myzus persicae ve sera beyazsineği Trialeurodes vaporariorum üzerinde test edilmiştir. M. persicae’nin T. vaporariorum’a göre uygulamaya daha duyarlı olduğu bulunmuştur. M. persicae üzerinde BMAUM M6-4 ve BMAUM LD 2016'nın tek ve hormon ile birlikte uygulamasında sırasıyla %58, %67, %95 ve %95 ölüm oranı görülmüştür. Aynı şekilde T. vaporariorum’a bu funguların tek ve hormon ile birlikte uygulanması sonucu sırasıyla %44, %68 ve %45, %78 oranında ölüm gözlenmiştir. M. persicae ve T. vaporariorum'da Bovine Serum Albumin (BSA) standardı ile 595 nm'de ölçülen protein seviyesi, BMAUM M6-4+AKH uygulamasında en yüksek besin mobilizasyonunu ortaya çıkarmıştır. Adipokinetik hormon tedavisinde karbonhidrat (glikoz) düzeyinin kontrol grubuna göre biraz arttığı bulunmuştur En düşük glukoz düzeyi entomopatojen fungus uygulamasında ölçülmekle birlikte, en yüksek düzey adipokinetik hormon tedavisi ile birlikte B. bassiana izolatlarında saptanmıştır. Entomopatojen funguslarla uygulanan AKH'nin etki mekanizması tam olarak anlaşılamamıştır. Bu nedenle, böceklere karşı gelecekteki entegre yönetim stratejileri için bu alanda yapılacak daha ayrıntılı çalışmalara ihtiyaç vardır.

Keywords

Adipokinetik hormon, Beauveria bassiana, Entomopatojen fungus, Myzus persicae, Trialeurodes vaporariorum

Project Number

No. 218O240

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