INVESTIGATION ANTIOXIDANT, ANTIMICROBIAL EFFECT AND PHENOLIC COMPOUNDS OF Alchemilla holotrichia EXTRACTS IN DIFFERENT POLARITIES

Year 2024, , 99 - 108, 26.09.2024

Bülent Kaya , Yekta Artuvan , Fatma Caf , Yusuf Menemen

https://doi.org/10.46810/tdfd.1178350

Abstract

In this study, different polarity extracts of Alchemilla holotrichia were obtained with hexane, dichloromethane, ethyl acetate, methanol and water. Then, total antioxidant activity, DPPH free radical scavenging activity, metal chelating capacity, total reducing power, hydrogen peroxide scavenging, hydroxy radical scavenging activity of these extracts were determined.Total phenol content 67.63± 0.88 GAE/g extract in the highest ethyl acetate extract, total flavonoid content 14.82±1.02–14.82±0.03 mg CE/g extract in the highest ethyl acetate and dichloromethane extract, phenolic acid content 0.72±0.21 mg SAE /g extract in the highest-hexane content
Determination of Antimicrobial activity was used agar well diffusion method and the agar disk diffusion method. These methods was tested on Bacillus subtilis ATCC 6633, Escherichia coli ATCC25922, Staphylococcus aureus ATCC 29213, Candida albicans, Klebsiella pneumoniae EMCS, Saccharomyces cerevisiae. The ethyl acetate extract of Alchemilla holotrichia has emerged the highest activity. The highest antimibacterial effect on Escherichia coli (17 mm) in the following, Klebsiella pneumoniae (15 mm) has emerged. Bacillus subtilis was determined as the most resistant microorganism (8 mm).
Alchemilla holotrichia showed both antioxidant and antimicrobial activity best in ethyl acetate extract. In the study, it was observed that ethyl acetate, which is a partially polar solvent, dissolves aromatic substances in the aerial part of the plant better than non-polar solvents such as n-hexane and diethyl ether.

Keywords

Antimicrobial, Antioxidant, Anticytotoxity, Alchemilla holotrichia, Plant Extracts

References

• Demiroz T, Nalbantsoy A, Kose FA, Baykan S. Phytochemical composition and antioxidant, cytotoxic and anti-inflammatory properties of Psephellus goeksunensis (Aytaç & H. Duman) Greuter & Raab-Straube.S Afr J Bot. 2020; 130: 1-7.
• Jiangning G, Xinchu W, Hou W, Qinghua L, Kaishun B. Antioxidants from a Chinese medicinal herb – Psoralea corylifolia L. Food Chem. 2005; 91(2): 287-92.
• Kaya, B. Determination of anticytotoxic, antioxidant and antimicrobial activities of Alchemilla persica extracts in different polarities. TDFD. 2020; 9(2): 157-169.
• Yazıcı S.Ö. Optimization of the extraction conditions of phenolic compounds from Alchemilla vulgaris using response surface methodology. Gıda.2021; 46(4): 1040-1052.
• İnci Ş, Eren A, Kirbağ S. Determination of antimicrobial and antioxidant activity of Alchemilla alpina L. TURJAF. 2021; 9(12): 2260-2264.
• Zhao M, Ito Y, Tu P. Isolation of a novel flavanone 6-glucoside from the flowers of Carthamus tinctorium (Honghua) by high-speed counter-current chromatography. J Chromatogr A. 2005; 1090(1-2): 193-196.
• Gamez-Meza N, Noriega-Rodriguez JA, Medina-Juarez LA, Ortega-Garcia J, Cazarez-Casanova R, Angulo-Guerrero O. Antioxidant activity in soybean oil of extracts from Thompson grape bagasse. J Am Oil Chem Soc. 1999; 76(12): 1445-1447.
• Barros L, Calhelha RC, Vaz JA, Ferreira ICFR, Baptista P, Estevinho LM. Antimicrobial activity and bioactive compounds of Portuguese wild edible mushrooms methanolic extracts. Eur Food Res Technol. 2007; 225(2): 151-156.
• Koncic MZ, Kremer D, Gruz J, Strnad M, Bisevac G, Kosalec I, et al. Antioxidant and antimicrobial properties of Moltkia petraea (Tratt.) Griseb. flower, leaf and stem infusions. Food Chem Toxicol. 2010; 48(6): 1537-1542.
• Barros L, Calhelha RC, Vaz JA, Ferreira ICFR, Baptista P, Estevinho LM. Antimicrobial activity and bioactive compounds of Portuguese wild edible mushrooms. Eur. Food Res Technol. 2007; 225: 151–156.
• Hatano T, Kagawa H, Yasuhara T, Okuda T. 2 new flavonoids and other constituents in Licorice Root - Their relative astringency and radical scavenging effects. Chem Pharm Bull. 1988; 36(6): 2090-2097.
• Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jpn J Nutri. 1986; 44:307-315.
• Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis. 1989; 10(6): 1003-1008.
• Dinis TCP, Madeira VMC, Almeidam, LM. Action of phenolic derivates (acetoaminophen, salycilate, and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and peroxyl radicals scavengers. Arch Biochem Biophys. 1994; 315: 161-169.
• ,Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem. 1999; 269(2): 337-341.
• Berghe VA. Screening methods for antibacterial and antiviral agents from higher plants. Meth Plant Biochem. 1991; 6: 47-68.
• Aqil F, Ahmed I, Mehmood Z. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turk J Biol. 2006; 30: 177-183.
• Paixao, N., et al. (2007). "Relationship between antioxidant capacity and total phenolic content of red, ros?? and white wines." Food Chemistry 105: 204-214
• Kaya B, Artuvan Y. Investigation of antioxidant and antimicrobial effect, phenolic compounds of Alchemilla cimilensis ECJSE. 2016; 3(1): 27-54.
• Oreopoulou A, Tsimogiannis D, Oreopoulou V. Extraction of polyphenols from aromatic and medicinal plants: an overview of the methods and the effect of extraction parameters. In: Polyphenols in plants. Press Elsevier, Amsterdam, p. 243–259.
• Sözmen EU, Eruygur N, Akpolat A, Çetin MD, Durukan H, Demirbaş A, et al. Sivas ili doğal florasından toplanan sarı kantaron (Hypericum scabrum L.) ve aslan pençesi (Alchemilla mollis (Buser) Rothm) bitkilerinin bazı kalite kriterlerinin belirlenmesi. JIST. 2020; 10(2): 1410-1418.
• ByKirk L. Parkin, Owen R. Fennema. Fennema's Food Chemistry,4th ed. Boca Raton. CRC Press; 2007.
• Jayaprakasha GK, Singh RP, Sakariah KK. Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chem. 2001; 73: 285–290.
• Handelman GJ. The evolving role of carotenoids in human biochemistry. Nutrition 2001; 17(10): 818–22.
• Mortensen A, Skibsted LH. Relative stability of carotenoid radical cations and homologue tocopheroxyl radicals. A real time kinetic study of antioxidant hierarchy. FEBS Lett. 1997; 417: 261-266.
• Karatoprak GS, Ilgun S, Kosar M. Phenolic composition, anti-inflammatory, antioxidant, and antimicrobial activities of Alchemilla mollis (Buser) Rothm.Chem. Biodivers. 2017; 14: e1700150.
• Oktyabrsky O, Vysochina G, Muzyka N, Samoilova Z, Kukushkina TG. Smirnova G. Assessment of antioxidant activity of plant extracts using microbial test systems. J Appl Microbiol. 2009; 106: 1175–1183.
• Neuza Paixão, Rosa Perestrelo, José C. Marques, José S. Câmara, Relationship between antioxidant capacity and total phenolic content of red, rosé and white wines, Food Chemistry, Volume 105, Issue 1, 2007, Pages 204-214, ISSN 0308-8146, https://doi.org/10.1016/j.foodchem.2007.04.017.
• Acet T, Kadriye Özcan K. Determination of antioxidant and antimicrobial properties of lady's mantle (Alchemilla ellenbergiana) extracts. GUFBD. 2018; 8 (1): 113-121.
• Vlaisavljević S, Jelača S, Zengin G, Mimica-Dukić N, Berežni S, Miljić M, et al. Alchemilla vulgaris agg. (Lady's mantle) from central Balkan: antioxidant, anticancer and enzyme inhibition properties. RSC Adv. 2019; 9(64): 37474-37483.
• Ruch RJ, Cheng SJ, Klaunig JE. Spin trapping of superoxide and hydroxyl radicals. Methods Enzymol. 1984; 105: 198-209.
• Shon MY, Choi SD, Kahng GG, Nam SH, Sung NJ. Antimutagenic, antioxidant and free radical scavenging activity of ethyl acetate extracts from white, yellow and red onions. FCT. 2004; 42(4): 659-666.
• Shafaghat A. Chemical constituents, antioxidant and antibacterial activities of the hexane extract of Alchemilla sericata Reichenb. J Food Biochem. 2019; 43: 9–14.
• Boroja T, Mihailović V, Katanić J, Pan SP, Nikles S, Imbimbo P, et al. The biological activities of roots and aerial parts of Alchemilla vulgaris L. S Afr J Bot. 2018; 116: 175-184.
• Kaya E, Şeker Karatoprak G, Koşar M, Özbilge H, Başer KHC. Antimicrobial Properties of Alchemilla mollis, Ankara, Türkiye, 23 June- 26 September 2009, 9th International Symposium on Pharmaceutical Sciences (ISOPS-9).
• Denev P, Kratchanova M, Ciz M, Lojek A, Vasicek O, Blazheva D, Nedelcheva P, Vojtek L, Hyrsl P. Antioxidant, antimicrobial and neutrophil-modulating activities of herb extracts. Acta Biochim Plon. 2014; 61: 359–367.
• Bengü, A. Ş., Adnan, A. Y. N. A., Özbolat, S., Abdullah, T. U. N. Ç., Aykutoğlu, G., Çiftci, M., & Darendelioğlu, E. (2020). Content and antimicrobial activities of bingol royal jelly. TTDB, 7(2), 480-486.
• İlgün, S, Karatoprak GŞ. (Alchemilla mollis (Buser) Rothm bitkisi köklerinden elde edilen ekstirelerin antioksidan ve sitotoksik etkileri. MÜSBD. 2021; 14(2): 176-185.
• Ayna, A., Tunc, A., Özbolat, S. N., Bengü, A. Ş., Aykutoğlu, G., Canli, D., & Darendelioğlu, E. (2021). Anticancer, and antioxidant activities of royal jelly on HT-29 colon cancer cells and melissopalynological analysis. Turk J Bot, 45(8), 809-819