Hesperidinin iyonlaştırıcı radyasyonun neden olduğu karaciğer hasarı üzerine koruyucu etkileri

Year 2019, , 498 - 504, 31.12.2019

Bengü Gülhan Aydın , Özcan Pişkin , Yılmaz Baş , Kemal Karakaya Özlem Elmas Murat Can , Çağatay Büyükuysal

https://doi.org/10.18663/tjcl.538024

Abstract

Amaç

Karaciğer
çoğunlukla üst batın, sağ alt akciğer, distal özafagus tümörleri için veya tüm
vücut radyoterapi (RT) uygulamasında radyasyona maruz kalmaktadır. Bu çalışmada
iyonizan radyasyonun karaciğer dokusunda neden olduğu oksidatif stresi üzerine
hesperidinin koruyucu etkisinin araştırılması amaçlanmıştır.

Gereç ve Yöntemler

24
yetişkin erkek rat rastgele 4 gruba ayrıldı. Kontrol grubuna sadece
fizyolojik salin, Grup HES’e 15 gün 50 mg/kg hesperidin, Grup RAD’a sadece
irradyasyon yapıldı ve Grup HES + RAD’a 15 gün boyunca 50 mg/kg hesperidin
verildi, 15. gün sonunda abdominopelvik bölgeye 10 Gy dozunda radyasyon
uygulandı. Radyasyon uygulandıktan
24 saat sonra Total antioksidan kapasite (TAK) ve malondialdehid (MDA) tayini
için karaciğer ve kan örnekleri alındı ve ayrıca histopatolojik inceleme
yapıldı.

Bulgular

Grup RAD ile karşılaştırıldığında, plazma ve doku MAD
düzeyi Grup HES + RAD'da anlamlı olarak azaldı (p=0.002).
Hem plazma hem de dokudaki
TAK, HES + RAD grubunda anlamlı olarak daha yüksek bulundu  (sırasıyla, p = 0.002, p = 0.004).Grup
RAD’da , histolojik olarak portal alanda ödem, sinüzoitlerde dilatasyon,
hepatositlerde belirgin olarak şime, intrasitoplazmik vakuolizasyon ,arada
nekroz, belirgin sinüzoidal dilatasyon, santral ven dilatasyonu ve konjesyon
izlendi. Nükleer hipertrofi belirgindi. Grup HES+RAD, Grup RAD ile
karşılaştırıldığında periportal ödem, santral ven dilatasyonu ve konjesyon
histolojik olarak belirgin değildi

Sonuç

Radyoterapinin
lipit peroksidasyonunda artışa ve antioksidan kapasitede azalmaya neden olduğu;
Ratlarda 15 gün boyunca 50 mg/kg/gün hesperidin uygulamasının, radyasyonun
neden olduğu karaciğer hasarında görülen histolojik değişiklikleri ve oksidatif
stresi azalttığı görülmüştür.

Keywords

Hesperidin, karaciğer hasarı, oksidatif stres

Protective effects of hesperidin on ionizing radiation-induced liver damage

Abstract

Aim: Liver
is mostly exposed to radiation during radiotherapy to the upper abdomen, the
right lobe of the lung, distal esophagus tumors or total body irradiation.
Radiation may lead to cellular damage, and clinical and laboratory findings of
liver dysfunction. This study aimed to
investigate the protective effect of peroral hesperidin on reducing oxidative
stress in liver tissue  caused by
ionızing radiation

Material and Methods: 24
adult male rats were randomly divide into four groups. Group control
was given only physiological saline, Group HES was given hesperidin at 50 mg/kg
body weight (BW) for 15 days, Group RAD was given only irradiation, and Group
HES+RAD was given hesperidin at 50 mg/kg BW daily and then irradiated. At the
end of 15 hesperidinin days,
the animals in Groups RAD and HES+RAD were exposed to a dose of 10 Gy to the
abdominopelvic region. Liver and blood samples were used for determination of total
antioxidant status (TAS) and malondialdehyde (MDA) and also histopathological
examination was performed.

Results: Compared
with the Group RAD, the plasma and tissue MAD level was significantly decreased
in Group HES+RAD (p=0.002). Both plasma and tissue levels of TAS was found
significantly higher in HES+RAD group (respectively, p=0.002,p=0.004). Histological
examination of Group RAD, portal edema, significant intra-cytoplasmic
vacuolization, swelling in the hepatocytes, necrosis, significant sinusoidal and
 central vein dilation and congestion
were observed . In group HES+RAD periportal edema, central vein dilation and
congestion were not histologically evident when compared with Group RAD.

Conclusion: Radiotherapy was found to lead to an increase in lipid
peroxidation and a reduction in anti-oxidant capacity; 50 mg/kg/day hesperidin
administration for 15 consecutive days was seen to reduce the histological
changes of liver damage and oxidative stress in rats 

Keywords

Hesperidin, liver damage, oxidative stress, radiation

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