Acute effect of moderate exercise on oxidative stres in smoker versus non-smokers

Year 2018, Volume: 9 Issue: 1, 55 - 58, 22.03.2018

Banu Çaycı Berrin Gunaydin , Seher Yuksel Sibel Soylemez , Çağrı Altundarak

https://doi.org/10.18663/tjcl.394776

Abstract

Aim: One of the particular
sources of oxidative stress is smoking and thiobarbituric acid reactive
substances (TBARS) and malondialdehyde (MDA) have been used as biomarkers of lipid
peroxidation of oxidative damage. Therefore, we aimed to investigate the acute
effect of moderate exercise on oxidative stress by determining serum MDA levels
by TBARS in moderate smoker subjects versus non-smokers.

Material & Methods: Fifty
healthy subjects performing moderate intensity exercise were assigned to 2
groups as moderate smokers (11-20 cigarettes/day) (Group Smoker, n=25) and
non-smokers (Group Non-Smoker, n=25). Venous blood samples were collected from
all participants half an hour before exercise (pre-exercise) and immediately
after exercise (post-exercise) to determine MDA levels as an indicator of lipid
peroxidation in the serum by TBARS/UV (ultra violet).

Results: Although no significant
differences were observed in pre-exercise MDA levels between the groups,
post-exercise MDA levels in smokers were significantly higher than that of
non-smokers (p<0.05).

Conclusion: The MDA
determination in serum by TBARS/UV appears to be positively correlated with
smoking status in particularly female subjects. Therefore, it can be a promising
helpful tool in demonstrating the oxidative stress due to moderate exercise
particularly in smokers to reorganize a healthier life style.

Keywords

Malondialdehyde (MDA), Smoking, Exercise

Sigara içen ve içmeyen bireylerde orta derecede egzersizin oksidatif stres üzerine akut etkileri

Abstract

Amaç:  Oksidatif stresin esas kaynaklarından biri sigara içilmesidir ve tiyobarbitürik asit reaktif türevleri (TBRAT) ve malondialdehit (MDA) oksidatif hasarın biyobelirteci olarak kullanılmıştır. Bu nedenle sigara içen ve içmeyen bireylerde TBART ile serumda MDA seviyelerini belirleyerek orta derecede egzerzisin akut etkilerini araştırmayı amaçladık.

Gereç ve Yöntemler: Orta derecede egzersiz yapan ve orta derecede sigara içen (11-20 sigara/gün)(Grup Sigara İçen, n=25) ve hiç sigara içmeyen (Grup Hiç Sigara İçmeyen, n=25) 50 sağlıklı birey iki gruba ayrıldı. Tüm bireylerden egzersizden yarım saat önce ve egzersizden hemen sonra TBART/UV ile serumda lipid peroksidasyonu indikatörü olarak MDA seviyelerini belirlemek için venöz kan örnekleri alındı. 

Bulgular: Gruplar arasında egzersiz öncesi MDA seviyelerinde anlamlı değişiklik olmamasına rağmen sigara içenlerde egzersiz sonrası MDA seviyeleri, sigara içmeyenlerden istatistiksel olarak anlamlı şekilde yüksek bulundu (p<0.05).

Sonuç: TBART/UV ile serumda MDA seviyelerinin belirlenmesi, özellikle kadın bireylerde sigara içilmesiyle pozitif ilişki göstermektedir. Bu nedenle özellikle sigara içenlerde daha sağlıklı bir hayat tarzını organize etmek için orta derecede egzersize bağlı oksidatif stresin gösterilmesinde ümit vaad eden yararlı bir araç olabilir.

Keywords

Malondialdehyde (MDA), Smoking, Exercise

References

• 1. Dryden GW, Deaciuc I, Arteel G, McClain CJ. Clinical implications of oxidative stress and antioxidant therapy. Curr Gastroeneterol Rep 2005; 7: 308-16.
• 2. Lykkesfeldt J. Malondialdehyde as biomarker of oxidative damage to lipids caused by smoking. Clinical Chimica Acta 2007; 380: 50-8.
• 3. Solak ZA, Kabaroglu C, Cok G, Parıldar Z, Bayindir U, Ozmen D, Bayindir O. Effect of different levels of cigarette smoking on lipid peroxidation, gluthathione enzymes and paranoxonase 1 activity in healthy people. Clin Exp Med 2005; 5: 99-105.
• 4. Mate-Munoz JL, Dominguez R, Barba M, Monroy AJ, Rodriguez B, Ruiz-Solano P, Garnacho-Castano MV. Cardiorespiratory and metabolic responses to loaded half squat exercise executed at an intensity corresponding to the lactate threshold. J Sports Sci Med 2015; 14: 648-56.
• 5. Pryor WA, Stone K. Oxidants in cigarette smoke. Radicals, hydrogen peroxide, peroxynitrate, and peroxynitrite. Ann N Y Acad Sci 1993; 686: 12-27.
• 6. Tudor-Locke C, Sisson SB, Collova T, Lee SM, Swan PD. Pedometer-determined step count guidelines for classifying walking intensity in a young ostensibly healthy population. Can J Appl Physiol 2005; 30: 666-76.
• 7. Gunaydin B, Sancak B, Candan S et al. Temporal variation of oxidant stress in critically ill patients. Minerva Anestesiol 2007; 73: 261-6.
• 8. Hunter MI, Nlemadim BC, Davidson DL. Lipid peroxidation products and antioxidant proteins in plasma and cerebrospinal fluid from multiple sclerosis patients. Neurochem Res 1985; 10: 1645-52.
• 9. Miller III ER, Appel LJ, Jiang L, Risby TH. Association between cigarette smoking and lipid peroxidation in a controlled feeding study. Circulation 1997;96:1097-101.
• 10. Sharma SB, Dwivedi S, Prabhu KM, Singh G, Kumar N, Lal MK. Coronary risk variables in young asymptomatic smokers. Indian J Med Res 2005; 122: 205-10.
• 11. Kalra J, Chaudhary AK, Prasad K. Increased production of oxygen free radicals in cigarette smokers. Int J Exp Pathol 1991; 72: 1-7.
• 12. Bolisetty S, Naidoo D, Lui K, Koh TH, Watson D, Montgomery R, Whitehall J. Postnatal changes in maternal and neonatal plasma antioxidant vitamins and the influence of smoking. Arch Dis Child Fetal Neonatal Ed 2002; 86: 36-40.