Sigara ile İndüklenen Böbrek Hasarına Vitamin E ve N-Nitro L-Arginin Metil Esterin (L-NAME) Koruyucu Etkisi

Year 2018, Volume: 3 Issue: 1, 205 - 209, 29.06.2018

Fahrettin Akyüz , Yakup Kara Fatih Kar , Ceyhan Hacıoğlu , Dilek Burukoğlu Dönmez

Abstract

Sigara, tütün bitkisinin yapraklarından elde edilen ve
birçok insan tarafından rahatlatıcı etkisi olduğuna inanılan bir maddedir.
Sigara bileşenlerinin, oksidatif stres maruziyeti nedeniyle böbrek fonksiyonu
üzerinde olumsuz etkileri olduğu bilinmektedir. E vitamini organizmayı çeşitli
mekanizmalarla koruyan ve hücresel boyutta hasarı önleyen önemli bir
antioksidan olarak bilinir. L-NAME nitrik oksit (NO) sentaz enzimini inhibe
ederek NO oluşumunu azaltır ve reaktif türlerin neden olduğu oksidatif hasarı
azaltmaya katkıda bulunur. Bu çalışmada, sigara maruziyetinin sıçan böbrekleri
üzerindeki etkisini ve E vitamini ile L-NAME'nin koruyucu etkilerini
araştırmayı amaçladık. Bu amaçla, kırk beş erkek albino sıçan beş gruba
ayrıldı: kontrol, sigara, sigara + E vitamini, sigara + L-NAME, sigara + E
vitamini + L-NAME. Sıçanlar, özel kafeslerde inhalasyon ile sigara dumanına
maruz bırakılmış ve 42 gün boyunca her gün tedavi gruplarına 200 mg/kg BW
Vitamin E ve/veya 50 mg/kg BW L-NAME intraperitoneal olarak uygulanmıştır. 42.
günün sonunda, sıçanların böbrek dokuları ve kanı biyokimyasal ve histolojik
analiz için alındı. Sıçan serumunda kreatinin ve kan üre azotu (BUN) ile böbrek
dokusu homojenatında malondialdehid (MDA), glutatyon (GSH) ve katalaz (CAT)
ölçümleri yapıldı. Sigaraya maruz kalan sıçanlarda serumda kreatinin, BUN ve
dokuda ise MDA düzeyleri anlamlı olarak artmışken, GSH düzeyleri ve CAT
aktivitesi anlamlı olarak azaldı. Serumda kreatinin, BUN, dokuda ise MDA, GSH
düzeyleri ve CAT aktivitesinin tedavi gruplarında normalize olduğu görüldü (p<0.05).
Histolojik incelemeler, tedavi gruplarında iyileşme olduğunu gösterdi. Bu
çalışmada sigara maruziyetinden kaynaklanan böbrek hasarının, E vitamini ve
L-NAME kullanımı ile düzeltilebileceği görülmüştür.

Keywords

Sigara, Böbrek hasarı, L-NAME, vitamin E

Protective Effect of Vitamin E and N-Nitro L-Arginine Methyl Ester (L-NAME) on Cigarette Induced Kidney Damage

Abstract

The Cigarette is a material obtained from the leaves of a tobacco plant
and believed to be comforting by many people. Cigarette components are known to
have adverse effects on renal function because of oxidative stress exposure.
Vitamin E is known to be an important antioxidant that protects the organism
with various mechanisms and prevents damage at the cellular dimension. L-NAME
inhibits nitric oxide (NO) synthase, reducing NO formation and contributing to
the reduction of oxidative damage induced by reactive species. In this study,
we aimed to investigate the effects of cigarette exposure on rat kidneys and
protective effect of vitamin E and L-NAME. For this purpose, Forty five male
albino rats were divided into five groups: control, cigarette, cigarette +
vitamin E, cigarette + L-NAME, cigarette + vitamin E + L-NAME. The rats were
exposed to cigarette smoke by inhalation in special cages and 200 mg/kg BW
Vitamin E and/or 50 mg/kg BW L-NAME administered intraperitoneal for 42 days.
These procedures were applied every day. At the end of the 42^nd day,
the kidney tissues and blood of rats were taken for biochemical and
histological analysis. Malondialdehyde (MDA), glutathione (GSH) and catalase
(CAT) measurements were performed on the kidney tissue homogenate, creatinine
and blood urea nitrogen (BUN) were measured in the rat serum. While serum
creatinine, BUN and tissue MDA levels significantly increased, GSH levels and
CAT activity decreased significantly in cigarette-exposed rats. Serum
creatinine, BUN, MDA, GSH and CAT levels were normalized in the treatment
groups (p<0.05). Histological examinations showed that there was improvement
in the treatment groups. It has been observed that kidney damage resulting from
cigarette-exposure can be eliminated by the use of vitamin E and L-NAME.

Keywords

Cigarette, Kidney damage, L-NAME, vitamin E

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