Deltamethrin and Imidacloprid Resistance Levels Of Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) Populations Collected From Afyonkarahisar, Turkey

Year 2020, Volume: 2 Issue: 1, 1 - 5, 25.06.2020

Cenk Keskin , Sibel Yorulmaz Salman

Abstract

Colorado potato beetle Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) is an important pest that can develop resistance to insecticides. In this study, imidacloprid and deltamethrin resistance levels were determined in ten Colorado potato beetle (CPB) populations collected from Afyonkarahisar province, which is one of the important potato production areas in Turkey, during the season of 2018. The third instar larvaes and topical application method were used in the bioassay experiments. For each experiment, five different concentrations of the insecticides were prepared by several dilutions of distilled water. Concentrations were applied to the thorax region of the larvae as 1 µl by micropipette. Only distilled water was applied to the control. The dead live counts were made after seventy-two hours and LD50 values of the populations were determined. Resistance ratios of imidacloprid and deltamethrin were calculated by the ratio of LD50 values of field populations to LD50 of susceptible population. Resistance rates determined for imidacloprid in Sandikli-1, Sandikli-2, Sandikli-3, Suhut-1, Suhut-2, Suhut-3, Dinar, Central, Bolvadin, Emirdag populations are 3.96, 7.43, 11.33, 14.33, 27.31, 25.08, 9.12, 2.34, 7.46, 8.72-fold, respectively; and 25.86, 36.50, 35.09, 60.11, 77.17, 72.82, 40.07, 9.41, 45.86, 56.59-fold, respectively for deltamethrin. As a result, high insecticide resistance was determined in CPB populations collected from potato production areas in Afyonkarahisar province. It is thought that insecticide resistance management protocols should be implemented in CPB control in these areas.

Keywords

Leptinotarsadecemlineata, LD50, imidacloprid, deltamethrin, insecticide resistance

Supporting Institution

Süleyman Demirel Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

FYL-2018-6054

Thanks

We would like to thank Suleyman Demirel University, Scientific Research Projects Coordination Unit for financing this project with FYL-2018-6054.

Afyonkarahisar İlinden Toplanılan Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) Popülasyonlarında Deltamethrin ve İmidacloprid Direnç Seviyeleri

Abstract

Patates böceği Leptinotarsadecemlineata (Say) (Coleoptera: Chrysomelidae) pestisitlere karşı direnç geliştirebilen önemli bir tarımsal zararlıdır. Bu çalışmada ülkemizin önemli patates üretim merkezlerinden biri olan Afyonkarahisar ilinden 2018 yılı üretim sezonunda toplanan 10 adet patates böceği popülasyonunda imidacloprid ve deltamethrin direnç düzeyleri belirlenmiştir. Bioassay denemelerde 3. Dönem larvalar üzerinde topical aplikasyon uygulaması yapılmıştır. Ölü canlı sayımları uygulamadan 72 saat sonra yapılmış ve LD50değerleri belirlenmiştir. Popülasyonların direnç oranları arazi popülasyonlarının LD50 değerlerinin, hassas popülasyonun LD50değerine oranlanmasıyla bulunmuştur. Sandıklı-1, Sandıklı-2, Sandıklı-3, Şuhut-1, Şuhut-2, Şuhut-3, Dinar, Merkez, Bolvadin, Emirdağ popülasyonlarınınimidacloprid için belirlenen direnç oranları sırasıyla 3.96, 7.43, 11.33, 14.33, 27.31, 25.08, 9.12, 2.34, 7.46, 8.72-kat; deltamethrin için belirlenen direnç oranları ise sırasıyla 25.86, 36.50, 35.09, 60.11, 77.17, 72.82, 40.07, 9.41, 45.86, 56.59-kat olarak tespit edilmiştir. Çalışma sonunda Afyonkarahisar ilinde patates böceği populasyonlarına göre farklı oranlarda insektisit direnci geliştiği belirlenmiştir. Sonuç olarak bölgede insektisit direnç yönetim proglamalarına uygun savaşım yöntemleri uygulanmalıdır.

Keywords

Leptinotarsa decemlineata, LD50, imidacloprid, deltamethrin, insektisit direnci

Project Number

FYL-2018-6054

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