The presence and prevalence of Enterococcus faecalis and Enterococcus faecium strains in urine and stool samples

Year 2018, Volume: 2 Issue: 1, 14 - 18, 30.04.2018

İsmail Hakkı Ekın , Sevil Ates Gülşah Tollu Ozgul Gulaydın Kemal Gurturk , Ziya Ilhan , Cihat Ozturk

Abstract

Objective: In this study, it was aimed to investigate the presence and the prevalence of Enterococcus
faecalis and Enterococcus faecium strains isolated from the urine and stool samples.

Materials and Methods: A total of 500 routine urine and feces samples were used for testing as the
study materials, and a total of 349 Enterococcus spp. were collected for investigation. For the isolation,
blood agar and bile esculin agar were used. DNA isolations of the 24-hour growth cultures of possible
enterococci were carried out using a DNA isolation kit.

Results: Out of 350 routine urine and 150 stool samples taken with the approval of the patients, 235
(67.1%) and 114 (76%) Enterococcus spp. were isolated respectively. Using the multiplex PCR method
with species specific primers, 136 (57.8%) of urine and 22 (19.2%) of stool originated enterococcal
strains were identified as Enterococcus faecalis; on the other hand, 17 (7.2%) of urine and 61 (53.5%)
of stool originated enterococci were identified as Enterococcus faecium.

Conclusion: As a result of the study in Van, Turkey, the isolation rate of Enterococcus faecalis and
Enterococcus faecium strains were found to be lower than other regions.

Keywords

Stool, Enterococcus faecium, Enterococcus faecalis, prevalance, urine

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