Investigating wound healing and antimicrobial activity of terebinth extract and terebinth extract+oxytetracycline mixture in experimental wounds in mice

Year 2024, Volume: 8 Issue: 1, 53 - 61, 01.04.2024

Nihat Şındak , Ali Gülaydın , Özgül Gülaydın , Mustafa Barış Akgül , Doğukan Özen

https://doi.org/10.47748/tjvr.1376617

Abstract

Objevtive: The aim of the study is to investigate the wound healing and antibacterial activity of terebinth extract and the mixture of terebinth + 3% oxytetracycline in experimental wounds in mice.
Material-Method: The animal material of the study consisted of 18 mice. The animals were divided into 3 groups as control group (group I, n: 6), terebinth group (group II, n: 6), terebinth +oxytetracycline group (group III, n: 6). Wounds with a 1-cm2 diameter were induced on the back of the mice and infected with Staphylococcus aureus ATCC® 25923 reference strain. Treatment protocols of the groups were applied daily. Total aerobic mesophilic bacteria and S. aureus count was performed in the swab samples taken on days 3, 7, and 14 of the healing process.
Results: In the study, it was found that wound healing process was completed the earliest in Group III (mean duration of 15.67±0.609 days), which was followed by Group II (18±0.73) and Group I (24.67±0.919), respectively. The healing period was statistically significantly shorter in Group II and Group III than in Group I (p<0.001). In the evaluation of aerobic mesophilic bacteria and S. aureus load, much less live bacteria were found in Group III compared to the other groups. In addition, the bacteria count in group II, in which terebinth extract was used, was less than that of the control group.
Conclusion: Consequently, it was concluded that the extract of terebinth plant grown in Siirt region reduced the bacterial load in the wound area and accelerated the healing process.

Keywords

mice, wound healing, Terebinth extract, Oxytetracycline, Antibacterial

Ethical Statement

The current study was approved by Siirt University Experimental Animals Local Ethics Committee with the decision dated 10.02.2017 and numbered 2017/01/21.

Supporting Institution

Siirt Üniversitesi Bilimsel Araştırmalar Koordinatörlüğü

Project Number

2017-SIUVET-24

References

• Akdoğan C, Özturan YA, Üner AG, Kalkan Y, Akın İ. Farelerde sarı kantaron (Hypericum perforatum) yağının yara iyileşme üzerine etkilerinin araştırılması. Türk Vet Cer Derg. 2022; 1(1):1-7.
• Akgül MB, Şındak N, Karakoç Z, Gülaydın A. Topikal olarak uygulanan menengiç yağı ve gliserin solüsyonun Japon Bıldırcınlarında (Coturnix Coturnix Japonica) yara iyileşmesi üzerine etkileri. Harran Üniv Vet Fak Derg. 2016; 5(2):146-151.
• Augusto BA, Alves PMS. Tetracycline: production, waste treatment and environmental impact assessment. Braz J Pharm Sci. 2014; 50(1):25-40.
• Ayla Ş, Günal MY, Şakul AA, Biçeroğlu Ö, Özdemir EM, Okur ME. Effects of Prunus Spinosa L. Fruits on experimental wound healing. Medeniyet Medical Journal. 2017; 32(3):152-158.
• Ayyanar M, Ignacimuthu S. Herbal medicines for wound healing among tribal people in Southern India: Ethnobotanical and Scientific evidences. IJARNP. 2009; 2:29-42.
• Balestrin LA, Back PI, Marques MS, et al. Effect of hydrogel containing achyrocline satureioides (asteraceae) extract–loaded nanoemulsions on wound healing activity. Pharmaceutics. 2022; 14(12):2726.
• Biswas TK, Mukherjee B. Plant medicines of ındian origin for wound healing activity: A Review. Lower Extr Wounds. 2003; 2:25-39.
• Budovsky A, Yarmolinsky L, Ben‐Shabat S. Effect of medicinal plants on wound healing. Wound Repair Regen. 2015; 23:171-183.
• Chitturi RT, Balasubramaniam AM, Parameswar RA, Kesavan G, Haris KTM, Mohideen K. The role of myofibroblasts in wound healing, contraction and its clinical implications in cleft palate repair. J Int Oral Health. 2015; 7:75-80.
• Christine L, Theoret DM. Diplomate ACVS; update on wound repair. Clin Tech Equine Pract. 2004; 3:110-112.
• Darby IA, Laverdet B, Bonté F, Desmoulière A. Fibroblasts and myofibroblasts in wound healing. Clin. Cosmet. Investig Dermatol. 2014; 7:301-311.
• Darwin E, Tomic-Canic M. Healing chronic wounds: Current challenges and potential solutions. Curr Dermatol Rep 2018; 7:296-302.
• De Almeida BM, Dorta dos Santos ID, De Carvalho FMA, et al. Himatanthus bracteatus-composed ın situ polymerizable hydrogel for wound healing. Int J Mol Sci. 2022; 23:15176.
• Demirseren DD. Topikal antibiyotikler (Çeşitleri, Etki Mekanizmaları) In: Türsen Ü, Karadağ AS, eds. Deri Hastalıklarında Topikal Antibiyotikler. Ankara: Akademisyen Kitabevi A.Ş; 2020. p.3-7.
• Dhifi W, Mnif W, Ouerhani B, Ghrissi K. Chemical composition and antibacterial activity of essential oil from the seeds of Pistacia terebinthus grown in Tunisia. J Essent Oil-Bear Plants. 2012; 15:582-588.
• Djerrou J, Maameri Z, Hamdo-Pacha Y, et al. Effect of virgin fatty oil of Pistacia lentiscus on experimental burn wound’s healing in rabbits. Afr J Tradit Complement Altern Med. 2010; 7:258-263.
• Gerçeker Türk B. Cerrahi işlem sonrası topikal antibiyotikler. In: Türsen Ü, Karadağ AS, eds. Deri Hastalıklarında Topikal Antibiyotikler. Ankara: Akademisyen Kitabevi A.Ş; 2020. p.123-128.
• Göçer H. Antıoxidant properties of terebinth (Pistacıa terebin thus l.) seeds. Int J Acad Res. 2013; 5:120-124.
• Hacıbekiroğlu I, Köseoğlu YP, Haimi N, Kılınç E, Tolan V, Kolak U. In vitro biological activities and fatty acid profiles of Pistacia terebinthus fruits and Pistacia khinjuk seeds. Nat Prod Res. 2015; 29:444-446.
• Ibrahim N, Wong SK, Mohamed IN, et al. Wound healing properties of selected natural products. Int J Environ Res Public Health. 2018; 15:2360.
• Kawashty A, Mosharrata SA, Saleh NM. The flavonoids of four Pistacia species in Egypt. Biochem Syst Ecol. 2000; 28:915-917.
• Khalil EA, Afifi FU, Al-Hussaini M. Evaluation of the wound healing effect of some Jordanian traditional medicinal plants formulated in Pluronic F127 using mice (Mus musculus). J Ethnopharmacol. 2007; 109:104-112.
• Kumar B, Vijayakumar M, Govindarajan R, Pushpangadan P. Ethnopharmacological approaches to wound healing-Exploring medicinal plants of India. J Ethnopharmacol. 2007; 114:103-113.
• Kusmenoglu S, Baser KHC, Ozek T. Constituents of the essential oil from the hulls of Pistacia vera L. J Essent Oil Res. 1995; 7:441-442.
• Lin TS, AbdLatiff A, Abd Hamid NA, WaNgah WZ, Mazlan M. Evaluation of topical tocopherol cream on cutaneous wound healing in streptozotocin-induced diabetic rats. Evid Based Complement Alternat Med. 2012; 49:10-27.
• Lucas Y, Niri R, Treuillet S, Douzi H, Castaneda B. Wound size ımaging: ready for smart assessment and monitoring. Adv Wound Care. 2021; 10(11):641-661.
• Mustoe TA, O’Shaughnessy K, Loeters O. Chronic wound pathogenesis and current treatment strategies: a unifying hypothesis. Plast Reconstr Surg. 2006; 117:35-41.
• Saco M, Howe N, Nathoo R, Cherpelis B. Topical antibiotic prophylaxis for prevention of surgical wound infections from dermatologic procedures: a systematic review and meta-analysis. J Dermatolog Treat. 2015; 26(2):151-158.
• Sharma A, Khanna S, Kaur G, Singh I. Medicinal plants and their components for wound healing applications. Future J Pharm Sci. 2021; 7:53.
• Sorg H, Tilkorn DJ, Hager S, Hauser J, Mirastschijski U. Skin wound healing: An Update on the current knowledge and concepts. Eur Surg Res. 2017; 58:81-94.
• Şındak N, Akgül MB, Gülaydın A, Karakoç Z. Effects of topical terebinth berry oil and different experimental mixtures on wound healing in Japanese Quails (Coturnix Coturnix Japonica). Van Vet J. 2017; 28(2):69-74.
• Taş A, Atasoy N, Özbek H, Aslan L, Yüksel H, Ceylan E, Dagoglu G. The Effects of Sildenafil Citrate (Viagra) in the Early Phase of Healing Process in Open Wounds in Dogs A. Acta Vet Brno. 2003; 72: 273–277.
• Thangapazham RL, Sharad S, Maheshwari RK. Phytochemicals in wound healing. Adv Wound Care. 2016; 5:230-241.
• Tohidi M, Khayami M, Nejati V, Meftahizade H. Evaluation of antibacterial activity and wound healing of Pistacia atlantica and Pistacia khinjuk. J Med Plant Res. 2011; 5(17):4310-4314.
• Türk Standardı (TS) 2001. Gıda ve hayvan yemleri mikrobiyolojisi-koagulaz-pozitif stafilokokların (Staphylococcus aureus ve diğer türler) sayımı için yatay metot- Bölüm 1- Baird-Parker Agar besiyeri kullanarak TS 6582-1 (ISO6888-1). Ankara: Türk Standardları Enstitüsü.
• Türk Standardı (TS) 2014. Gıda zinciri mikrobiyolojisi-Mikroorganizmaların sayımı için yatay yöntem Bölüm 1: Dökme plak tekniğiyle 30°C’ta koloni sayımı (ISO 4833-1:2013). Ankara: Türk Standardları Enstitüsü.
• Wallace HA, Basehore BM, Zito PM. Wound healing phases. In: StatPearls. Treasure Island, FL, USA: StatPearls Publishing LLC; 2022.
• Wang PH, Huang BS, Horng HC, Yeh CC, Chen YJ. Wound healing. J Chin Med Assoc. 2018; 81:94-101.
• Wernick B, Nahirniak P, Stawicki SP. Impaired wound healing. In: Stat Pearls. Treasure Island: Publishing LLC; 2022.
• Ximenes RM, de Morais Nogueira L, Cassundé NM, et al. Antinociceptive and wound healing activities of Croton adamantinus Müll. Arg. essential oil. J Nat Med. 2013; 67:758-764.
• Yuan H, Ma Q, Ye L, Piao G. The traditional medicine and modern medicine from natural products. Mol. 2016; 21(5):559.