Purification of Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) From Fish Oil Using HPLC Method and Investigation of Their Antibacterial Effects on Some Pathogenic Bacteria

Year 2019, Volume: 5 Issue: 1, 35 - 43, 01.11.2019

Yılmaz Uçar , Fatih Özoğul , Mustafa Durmuş , Yeşim Özoğul , Ali Rıza Köşker Esmeray Küley Boğa , Deniz Ayas

Abstract

The aim of this study
was to purified eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)
essential oils from trout oil using high performance liquid chromatography
(HPLC) method, and bioconverted EPA and DHA into bioconverted EPA (bEPA) and
bioconverted DHA (bDHA) extracts by P.
aeruginosa PR3. Moreover, in vitro antibacterial activity of bEPA and bDHA
was investigated using disc diffusion methods and minimum inhibitory
concentration (MIC). EPA and DHA concentration of 11.1% and 15.9% in trout oil
increased in 58.64% and 40.33% after HPLC optimisation, respectively. In this
study, EPA and DHA enriched products were obtained which are to be used as
valuable supplements for food and pharmaceutical purposes. The bioconverted EPA
and DHA exhibited antibacterial activities against two Gram-positive bacteria (Listeria monocytogenes ATCC 7677 and Staphylococcus aureus ATCC 29213) and
six Gram-negative bacteria (Pseudomonas
aeruginosa ATCC 27853, Escherichia
coli ATCC 25922, Klebsiella
pneumoniae ATCC700603, Enterococcus
faecalis ATCC 29212, Aeromonas
hydrophila NCIMB 1135 and Salmonella paratyphi
A NCTC 13). Inhibition zones and MIC value of bEPA and bDHA against bacterial
strains ranged from 7 to 12 mm and from 350 to 2350 μg/mL, respectively. Our
results suggested that the crude extracts of bioconversion of EPA and DHA by P. aeruginosa PR3 can be considered as
promising antimicrobials in improving food safety by controlling foodborne
pathogens.

Keywords

High performance liquid chromatography (HPLC), docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), minimum inhibitory concentration (MIC)

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